| 研究実績の概要 |
In the first year, we designed magnetic tweezers for constructing 3D hierarchical vascular networks in a cm-sized liver tissue. In addition, we fabricated several patterns of vascular network in cellular structure in order to compare the efficiency of the vascular networks. For this, we fabricated several patterns of vascular networks; without vascular network, with vertical channel, and with 3D hierarchical channels. After 3 weeks, the cell viability of four samples were analyzed and we found that the tissue having 3D hierarchical channels show higher cell viability than others. From the result, we can see that the fabricated 3D hierarchical channels can supply the nutrient and oxygen to the cells in tissues and improve the cell condition after a long-term culture..
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| 今後の研究の推進方策 |
In the next step, we will apply perfusable network within 3D cellular structure by adding circulation system and then to transform from the empty channels to vascular network, I will seed human umbilical vein endothelial cells (HUVECs) to the channels and perfuse the culture medium to the vascular networks. Finally, we will analyze cell condition and liver function of constructed tissue.
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