Functional analysis of host factors involved in antiviral defense networks in plants

• Project/Area Number: 20688002
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Plant pathology
• Research Institution: Hokkaido University
• Principal Investigator: NAKAHARA Kenji 北海道大学, 大学院・農学研究院, 助教 (90315606)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥25,610,000 (Direct Cost: ¥19,700,000、Indirect Cost: ¥5,910,000); Fiscal Year 2011: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2009: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2008: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
• Keywords: PAMPs / RNAi / 自然免疫 / 植物ウイルス / RNAサイレンシング / カルモジュリン様タンパク / オートファジー / 植物自然免疫 / 生体防御 / 感染生理

Research Abstract

This study uncovered a novel mechanism of antiviral defense cooperatively acting with RNA silencing. In the defense, a calmodulin-like protein, rgs-CaM, binds to diverse viral RNA silencing suppressors and directs degradation of them by autophagy. Thus, the rgs-CaM-mediated defense seemed a host countermeasure against viral RNA silencing suppressors to reinforce antiviral RNA silencing in tobacco.

Research Products

• [Journal Article] Characterization of the recessive resistance gene cyv1 against Clover yellow vein virus in Pisum sativum (2012)
  • Author(s): Sun Hee Choi, Kenji S. Nakahara, Marcelo Andrade, Ichiro Uyeda
  • Journal Title: Journal of General Plant Pathology Volume: 78 Issue: 4 Pages: 269-276
  • DOI: 10.1007/s10327-012-0383-9
  • Peer Reviewed
• [Journal Article] Heterologous expression of viral suppressors of RNA silencing complements virulence of the HC-Pro mutant of Clover yellow vein virus in pea (2012)
  • Author(s): Go Atsumi1, Kenji S. Nakahara, Tomoko Sugikawa Wada, Sun Hee Choi, Chikara Masuta, and Ichiro Uyeda
  • Journal Title: Archives of Virology Volume: (In press)(印刷中) Issue: 6 Pages: 1019-1028
  • DOI: 10.1007/s00705-012-1281-3
  • Peer Reviewed
• [Journal Article] White clover mosaic virus-induced gene silencing in pea (2012)
  • Author(s): Yukari Ido, Kenji S. Nakahara, Ichiro Uyeda
  • Journal Title: Journal of General Plant Pathology Volume: 78 Issue: 2 Pages: 127-132
  • DOI: 10.1007/s10327-012-0360-3
  • Peer Reviewed
• [Journal Article] Characterization of the recessive resistance gene cyv1 against Clover yellow vein virus in Pisum sativum (2012)
  • Author(s): Choi, SH, et al.
  • Journal Title: Journal of General Plant Pathology Volume: (In press)(印刷中)(未定)
  • Peer Reviewed
• [Journal Article] Screening and analysis of genes expressed upon infection of broad bean with Clover yellow vein virus causing lethal necrosis (2011)
  • Author(s): Kenji S. Nakahara, Hiroaki Kitazawa, GoAtsumi, Sun Hee Choi, Yuji Suzuki, Ichiro Uyeda
  • Journal Title: Virology Journal Volume: 8 Issue: 1 Pages: 355-355
  • DOI: 10.1186/1743-422x-8-355
  • Peer Reviewed
• [Journal Article] Cargo sorting to lysosome-related organelles regulates siRNA-mediated gene silencing (2011)
  • Author(s): Dinari A. Harris, Kevin Kim, Kenji Nakahara, Constanza Vasquez-Doorman, and Richard W. Carthew
  • Journal Title: Journal of Cell Biology Volume: 194 Issue: 1 Pages: 77-87
  • DOI: 10.1083/jcb.201102021
  • Peer Reviewed
• [Journal Article] Sensitive PCR-based Detection of Apple Chlorotic Leaf Spot Virus Heterogeneous in Apple Trees (2011)
  • Author(s): Kenji S. Nakahara, Kouji Yoshida, Tsutae Ito, Kouichi Suzaki and Nobuyuki Yoshikawa
  • Journal Title: Japan Agricultural Research Quarterly: JARQ Volume: 45 Issue: 4 Pages: 411-421
  • DOI: 10.6090/jarq.45.411
  • NAID: 130002147955
  • ISSN: 0021-3551, 2185-8896
  • Peer Reviewed
• [Journal Article] Sensitive PCR-based detection of Apple chlorotic leaf spot virus heterogenous in apple trees (2011)
  • Author(s): Kenji S.Nakahara, Kouji Yoshida, Tsutae Ito, Kouichi Suzaki, Nobuyuki Yoshikawa
  • Journal Title: Japan Agricultural Research Quarterly in press
  • Peer Reviewed
• [Journal Article] Sensitive PCR-based detection of Apple chlorotic leaf spot virus heterogenous in apple trees (2011)
  • Author(s): Nakahara, K.
  • Journal Title: Japan Agricultural Research Quarterly Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Involvement of the P1 Cistron in Overcoming eIF4E-Mediated Recessive Resistance Against Clover yellow vein virus in Pea (2010)
  • Author(s): Kenji S. Nakahara, Ryoko Shimada, Sun-HeeChoi, Haruko Yamamoto, Jun Shao, and Ichiro Uyeda
  • Journal Title: Molecular Plant Microbe Interactions Volume: 23 Issue: 11 Pages: 1460-1469
  • DOI: 10.1094/mpmi-11-09-0277
  • Peer Reviewed
• [Journal Article] Involvement of the P1 Cistron in Overcoming eIF4E-Mediated Recessive Resistance Against Clover yellow vein virus in Pea (2010)
  • Author(s): Kenji S.Nakahara, Ryoko Shimada, Sun-Hee Choi, Haruko Yamamoto, Jun Shao, Ichiro Uyeda
  • Journal Title: Molecular Plant Microbe Interactions 23 Pages: 1460-1469
  • Peer Reviewed
• [Journal Article] Silencing by small RNAs is linked to endosomal trafficking (2009)
  • Author(s): Young Sik Lee, Sigal Pressman, Arlise P. Andress, Kevin Kim, Jamie L. White, Justin J. Cassidy, Xin Li1, Kim Lubell, Do Hwan Lim, Ik Sang Cho, Kenji Nakahara, Jonathan B. Preall1, Priya Bellare1, 5, Erik J. Sontheimer1 & Richard W. Carthew
  • Journal Title: Nature Cell Biology Volume: 11 Issue: 9 Pages: 1150-1156
  • DOI: 10.1038/ncb1930
  • Peer Reviewed
• [Journal Article] The cyv-2 resistance to Clover yellow vein virus in pea is controlled by the eukaryotic initiation factor 4E (2009)
  • Author(s): Marcelo Andrade, Yosuke Abe, Kenji S. Nakahara and Ichiro Uyeda
  • Journal Title: Journal of General Plant Pathology Volume: 75 Issue: 3 Pages: 241-249
  • DOI: 10.1007/s10327-009-0163-3
  • Peer Reviewed
• [Journal Article] Activation of the Salicylic Acid Signaling Pathway Enhances Clover yellow vein virus Virulence in Susceptible Pea Cultivars (2009)
  • Author(s): Go Atsumi, Uiko Kagaya, Hiroaki Kitazawa, Kenji S. Nakahara, and Ichiro Uyeda
  • Journal Title: Molecular Plant Microbe Interactions Volume: 22 Issue: 2 Pages: 166-175
  • DOI: 10.1094/mpmi-22-2-0166
  • Peer Reviewed
• [Journal Article] Preall1, Priya Bellare1, 5, Erik J.Sontheimer1 & Richard W.Carthew, Silencing by small RNAs is linked to endosomal trafficking (2009)
  • Author(s): Young Sik Lee, Sigal Pressman, Arlise P.Andress, Kevin Kim, Jamie L.White, Justin J.Cassidy, Xin Li1, Kim Lubell, Do Hwan Lim, Ik Sang Cho, Kenji Nakahara, Jonathan B.
  • Journal Title: Nature Cell Biology 11 Pages: 1150-1156
  • Peer Reviewed
• [Journal Article] The cyv-2 resistance to Clover yellow vein virus in pea is controlled by the eukaryotic initiation factor 4E (2009)
  • Author(s): Marcelo Andrade, Yosuke Abe, Kenji S.Nakahara, Ichiro Uyeda
  • Journal Title: Journal of General Plant Pathology 75 Pages: 241-249
  • Peer Reviewed
• [Journal Article] Activation of the Salicylic Acid Signaling Pathway Enhances Clover yellow vein virus Virulence in Susceptible Pea Cultivars (2009)
  • Author(s): Go Atsumi, Uiko Kagaya, Hiroaki Kitazawa, Kenji S.Nakahara, Ichiro Uyeda
  • Journal Title: Molecular Plant Microbe Interactions 22 Pages: 166-175
  • Peer Reviewed
• [Journal Article] Silencing by small RNAs is linked to endosomal trafficking (2009)
  • Author(s): Lee, Y.S.
  • Journal Title: Nat Cell Biol 11 Pages: 1150-1156
  • Peer Reviewed
• [Journal Article] The cyv-2 resistance to Clover yellow vein virus in pea is controlled by the eukaryotic initiation factor 4E (2009)
  • Author(s): Andrade, M.
  • Journal Title: J Gen Plant Pathol 75 Pages: 241-249
  • Peer Reviewed
• [Journal Article] Activation of the salicylic acid signaling pathway enhances Clover yellow vein virus virulence in susceptible pea cultivars (2009)
  • Author(s): Atsumi, G.
  • Journal Title: Molecular Plant Microbe Interactions 22 Pages: 166-175
  • Peer Reviewed
• [Journal Article] A tobacco calmodulin-like protein provides secondary defense by binding to and directing degradation of virus RNA silencing suppressors
  • Author(s): Kenji S. Nakahara, Chikara Masuta, SyoutaYamada, Hanako Shimura, Yukiko Kashihara, Tomoko S. Wada, Ayano Meguro, Kazunori Goto, Kazuki Tadamura, Kae Sueda, Toru Sekiguchi, Jun Shao, Noriko Itchoda, Takeshi Matsumura, Manabu Igarashi, Kimihito Ito, Richard W. Carthew, and Ichiro Uyeda
  • Journal Title: Proceedings of the National Academy of Sciences USA Volume: (印刷中)
  • URL: http://www.pnas.org/
  • Peer Reviewed
• [Presentation] タバコのウイルスに対する基礎免疫におけるカルモジュリン様タンパクrgs-CaMの役割2 (2012)
  • Author(s): 中原健二
  • Organizer: 平成24年度日本植物病理学会大会
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2012-03-29
• [Presentation] タバコのウイルスに対する基礎免疫におけるカルモジュリン様タンパクrgs-CaMの役割2 (2012)
  • Author(s): 中原健二
  • Organizer: 平成24年度日本植物病理学会大会
  • Place of Presentation: 福岡国際会議場(福岡)
  • Year and Date: 2012-03-29
• [Presentation] P3N-PIPOはcyv1エンドウに対するクローバ葉脈黄化ウイルスの病原性に関与する (2012)
  • Author(s): 崔善熹
  • Organizer: 平成24年度日本植物病理学会大会
  • Place of Presentation: 福岡国際会議場(福岡)
  • Year and Date: 2012-03-29
• [Presentation] RNAサイレンシングを強化するタバコのウイルス防御機構 (2011)
  • Author(s): 中原健二
  • Organizer: 北海道植物学会大会(招待講演)
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2011-12-02
• [Presentation] RNAサイレンシングを強化するタバコのウイルス防御機構 (2011)
  • Author(s): 中原健二
  • Organizer: 北海道植物学会大会
  • Place of Presentation: 北海道大学大学院地球環境科学研究院(札幌)(招待講演)
  • Year and Date: 2011-12-02
• [Presentation] An rgs-CaM-mediated countermeasure for RNAi-based antiviralimmunity in tobacco (2011)
  • Author(s): Kenji Nakahara
  • Organizer: IUMS, XV International Congress of Virology
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2011-09-13
• [Presentation] An rgs-CaM-mediated countermeasure for RNAi-based antiviral immunity in tobacco (2011)
  • Author(s): Nakahara, K
  • Organizer: IUMS, XV International Congress of Virology
  • Place of Presentation: 札幌コンベンションセンター(札幌)
  • Year and Date: 2011-09-13
• [Presentation] The mutations in P3 and P3N-PIPO of Clover yellow vein virus affect resisntance breaking in pea (2011)
  • Author(s): Choi, SH
  • Organizer: IUMS, XV International Congress of Virology
  • Place of Presentation: 札幌コンベンションセンター(札幌)
  • Year and Date: 2011-09-13
• [Presentation] タバコのウイルスに対する基礎免疫におけるカルモジュリン様タンパクrgs-CaMの役割1 (2011)
  • Author(s): 中原健二
  • Organizer: 平成23 年度日本植物病理学会大会
  • Place of Presentation: 東京農工大学(府中市)
  • Year and Date: 2011-03-29
• [Presentation] タバコのウイルスに対する基礎免疫におけるカルモジュリン様タンパクrgs-CaMの役割1 (2011)
  • Author(s): 中原健二
  • Organizer: 平成23年度日本植物病理学会大会
  • Place of Presentation: 東京農工大学(府中市)
  • Year and Date: 2011-03-29
• [Presentation] Genome analyses of P3 and P3N-PIPO of Clover yellow vein virus that breaks resistance in tentative cyv1 pea (2011)
  • Author(s): Choi, S.
  • Organizer: 平成23年度日本植物病理学会大会
  • Place of Presentation: 東京農工大学(府中市)
  • Year and Date: 2011-03-27
• [Presentation] Counterdefense against Viral RNAi Suppressors in Tobacco (2011)
  • Author(s): Nakahara, K.
  • Organizer: Keystone SYMPOSIA Mechanism and Biology of Silencing
  • Place of Presentation: Monterey, California, USA(招待講演)
  • Year and Date: 2011-03-24
• [Presentation] Counterdefense against Viral RNAi Suppressors in Tobacco (2011)
  • Author(s): Kenji Nakahara
  • Organizer: Keystone SYMPOSIA Mechanism and Biologyof Silencing (招待講演)
  • Place of Presentation: Monterey, California, USA
• [Presentation] Counterdefense ag ainst Viral RNAi Suppressors in Tob acco (2011)
  • Author(s): Kenji Nakahara
  • Organizer: Keystone SYMPOSIA Mechani sm and Biology of Silencing
  • Place of Presentation: Monter ey, California, USA(招待講演)
• [Presentation] Effect of rgs-CaM overexpression on PVY accumulation in transgenic tobacco plants (2010)
  • Author(s): Syota Yamada
  • Organizer: Keystone symposia RNA silencing mechanisms in plants
  • Place of Presentation: Santa Fe, New Mexico, USA
• [Presentation] Effect of rgs-CaM overexpression on PVY accumulation in transgenic to bacco plants (2010)
  • Author(s): Yamada, S.
  • Organizer: Keystone symposia(RNA silencing mechanisms in plants)
  • Place of Presentation: Santa Fe, New Mexico, USA
• [Presentation] A resistance gene in pea line PI429853 to Clover yellow vein virus in mapped to linkage group II and linked with cyv1 (2009)
  • Author(s): Choi, S.-H.
  • Organizer: The 1^<st> Japan-Korea joint symposium
  • Place of Presentation: Jeju, Korea
• [Presentation] Analysis of pea proteome during Clover yellow vein virus infection (2009)
  • Author(s): Yamada, S.
  • Organizer: The 1^<st> Japan-Korea joint symposium
  • Place of Presentation: Jeju, Korea
• [Presentation] クローバ葉脈黄化ウイルスはP1に点変異を生じることにより翻訳開始因子をコードするcyv-2遺伝子によるエンドウ劣性抵抗性を打破する (2009)
  • Author(s): 中原健二
  • Organizer: 平成21年度日本植物病理学会大会
  • Place of Presentation: 山形市、山形大学
• [Presentation] エンドウPl 226564系統においてクローバ葉脈黄化ウイルスの強毒性を決定するウイルス因子の探索 (2009)
  • Author(s): 厚見剛
  • Organizer: 平成21年度目本植物病理学会大会
  • Place of Presentation: 山形市、山形大学
• [Presentation] クローバ葉脈黄化ウイルスの感染で誘導されるエンドウ遺伝子の発現 (2008)
  • Author(s): 山田翔太
  • Organizer: 平成20年度日本植物病理学会大会北海道部会
  • Place of Presentation: 札幌市、北海道農業研究センター
  • Year and Date: 2008-10-17
• [Presentation] eukaryotic initiation factor 4E involved in a recessive resistance to Clover yellow vein virus in pea carrying cyv2 (2008)
  • Author(s): Kenji Nakahara
  • Organizer: International Congress of Virology, The International Union of Microbiological Societies
  • Place of Presentation: Istanbul, ISTANBUL CONVENTION AND EXHIBITION CENTRE
• [Presentation] eukaryotic initiation factor 4E involved in a recessive resistance to Clover yellow vein virus in pea carrying cyv2, International Congress of Virology (2008)
  • Author(s): Kenji Nakahara
  • Organizer: The International Union of Microbiological Societies
  • Place of Presentation: Istanbul, ISTANBUL CONVENTION AND EXHIBITION CENTRE
• [Presentation] eukaryotic initiation factor 4E involved in a recessive resistance to Clover yellow vein virus in pea carrying cyv2 (2008)
  • Author(s): Nakahara, K.
  • Organizer: International Congress of Virology, The INTERNATIONAL UNION OF MICROBIOLOGICAL SOCIETIES
  • Place of Presentation: Istanbul, ISTANBUL CONVENT ION AND EXHIBITION CENTRE
• [Presentation] クローバ葉脈黄化ウイルスの病原性に関わる宿主タンパク質の同定 (2008)
  • Author(s): 山田翔太
  • Organizer: 日本ウイルス学会北海道支部第42回夏期シンポジウム
  • Place of Presentation: ニセコ町、ホテルニセコいこいの村