Basic studies on development of a novel delivery system of materials into fish oocytes

• Project/Area Number: 23580243
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General fisheries
• Research Institution: Hokkaido University
• Principal Investigator: HIRAMATSU Naoshi 北海道大学, 大学院水産科学研究院, 准教授 (10443920)
• Co-Investigator(Kenkyū-buntansha): GEN Koichiro 独立行政法人水産総合研究センター, 西海区水産研究所, 主任研究員 (80372051)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 魚類 / 受容体標的輸送 / ビテロジェニン / ビテロジェニン受容体 / 蛋白質相互作用 / 卵母細胞

Research Abstract

A yolk precursor vitellogenin (Vg) is selectively incorporated from the maternal blood into oocyte following its binding to ovarian Vg receptor. This study demonstrated that Vg obtained from heterologous species, as well as Vg coupled with voluntary lipids, can be delivered to fish eggs and offsprings by injecting them into broodstocks. This indicated a potential use of fish Vg as a transporter of bioactive materials; the system can be universal to any fish species. This study also confirmed that such system was found to be applicable for the delivery of bioactive lipids and/or hydrophobic materials to eggs and offsprings across generations.

Research Products

• [Journal Article] Multiple ovarian lipoprotein receptors in teleosts (2013)
  • Author(s): Hiramatsu N, Luo W, Reading B. J., Sullivan C. V., Mizuta H, Ryu Y. -W., Nishimiya 0, Todo T, Hara A
  • Journal Title: Fish Physiology and Biochemistry Volume: 39 Issue: 1 Pages: 29-32
  • DOI: 10.1007/s10695-012-9612-6
  • Peer Reviewed
• [Journal Article] Ovarian expression and localization of a vitellogenin receptor with eight ligand binding repeats in the cutthroat trout (Oncorhynchus clarki) (2013)
  • Author(s): Mizuta, H., Luo, W., Ito, Y., Mushirobira, Y., Todo, T., Hara, A., Reading, B. J., Sullivan, C. V., Hiramatsu, N.
  • Journal Title: Comparative Biochemistry and Physiology-Part B Volume: 166 Issue: 1 Pages: 81-90
  • DOI: 10.1016/j.cbpb.2013.07.005
  • Peer Reviewed
• [Journal Article] カットスロートトラウトOncorhynchus clarkiにおける2型ビテロジェニン転写産物および蛋白質の卵発達に伴う発現変化 (2013)
  • Author(s): 筵平裕次、水田紘子、羅ブンシュ、盛田祐加、澤口小有美、松原孝博、平松尚志、東藤孝、原彰彦
  • Journal Title: 日本水産学会誌 Volume: 79(2) Pages: 175-189
  • NAID: 10031138611
  • URL: http://www.miyagi.kopas.co.jp/JSFS/kaishi.html
  • Peer Reviewed
• [Journal Article] カットスロートトラウト Oncorhynchus clarki における2型ビテロジェニン転写産物および蛋白質の卵発達に伴う発現変化 (2013)
  • Author(s): 筵平裕次、水田紘子、羅ブンシュ、盛田祐加、澤口小有美、松原孝博、平松尚志、東藤孝、原彰彦
  • Journal Title: 日本水産学会誌 Volume: 79(2) Pages: 175-189
  • NAID: 10031138611
  • Peer Reviewed
• [Presentation] Ovarian yolk formation in fishes : Molecular mechanisms underlying formation of lipid droplets and vitellogenin-derived yolk proteins (2014)
  • Author(s): Hiramatsu, N., Todo, T., Sullivan, C.V., Reading, B.J., Matsubara, T., Ryu, Y.-W., Mizuta, H., Luo, W., Nishimiya, O., Mushirobira, Y. and Hara, A.
  • Organizer: 10^<th> International Symposium on Reproductive Physiology of Fish (ISRPF)
  • Place of Presentation: Olhao, Portugal(基調講演として発表決定済)
• [Presentation] Ligand binding properties of ovarian lipoprotein receptors in the cutthroat trout (2014)
  • Author(s): Mushirobira. Y., Mizuta, H., Luo, W., Todo, T., Hara, A., Reading, B.J., Sullivan, C.V. and Hiramatsu, N.
  • Organizer: 10^<th> International Symposium on Reproductive Physiology of Fish (ISRPF)
  • Place of Presentation: Olhao, Portugal(ポスター発表として発表決定済)
• [Presentation] Ovarian yolk formation in fishes: Molecular mechanisms underlying formation of lipid droplets and vitellogenin-derived yolk proteins (2014)
  • Author(s): Hiramatsu, N., Todo, T., Sullivan, C.V., Reading, B.J, Matsubara, T., Ryu, Y-.W., Mizuta, H., Luo, W., Nishimiya, O., Wu, M., Mushirobira, Y., and Hara, A.
  • Organizer: 10th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: Olhao Municipal Auditorium, Olhao, Portugal
  • Invited
• [Presentation] Yolk formation in fish : Multiple vitellogenins and their receptors (2013)
  • Author(s): Hiramatsu N., Mizuta H., Luo W., Nishimiya O., Wu M., Mushirobira Y., Reading B.J., Sullivan C.V., Todo T. and Hara A.
  • Organizer: Diversification in Inland Finfish Aquaculture II (DIFAII)
  • Place of Presentation: University of South Bohemia, Vodnany, Czech Republic
• [Presentation] Yolk formation in fish: Multiple vitellogenins and their receptors (2013)
  • Author(s): Hiramatsu, N., Mizuta, H., Luo, W., Nishimiya, O., Wu, Meiqin, Mushirobira, Y., Reading, B.J., Sullivan, C.V., Todo, T., and Hara, A.
  • Organizer: Diversification in Inland Finfish Aquaculture II (DIFAII)
  • Place of Presentation: University of South Bohemia, Vodnany, Czech Republic
• [Presentation] Dual vitellogenins in cutthroat trout (Oncorhynchus clarki): purification and changes in serum protein level during reproductive cycle (2012)
  • Author(s): Mushirobira Y., Mizuta H., Luo W., Morita Y., Sawaguchi S., Matsubara T., Hiramatsu N., Todo T., Hara A.
  • Organizer: The 9th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
  • Place of Presentation: 東京
• [Presentation] 卵黄蛋白前駆物質ビテロジェニンの異種間投与とその運搬過程:イトウとゼブラフィッシュを用いたモデルについて (2011)
  • Author(s): 櫻井秀之・川北奈央子・平松尚志・東藤孝・原彰彦
  • Organizer: 第5回サケ学研究会
  • Place of Presentation: 北海道大学学術交流会館小講堂、札幌
• [Remarks] ホームページ
  • URL: http://www.geocities.jp/hlaboratory/index.html