The germline : its developmental cycle and epigenome network

2009 Fiscal Year Self-evaluation Report

• Project Area: The germline: its developmental cycle and epigenome network
• Project/Area Number: 19061001
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyushu University (2010-2012); National Institute of Genetics (2007-2009)
• Principal Investigator: SASAKI Hiroyuki National Institute of Genetics, 総合遺伝研究系, 研究員 (30183825)
• Project Period (FY): 2007 – 2012
• Keywords: 発生・分化 / 発現制御 / 生殖細胞 / エピゲノム / リプログラミング

Research Abstract

本領域は哺乳類の生殖系列の世代サイクルとエピゲノムネットワークを包括的に解明することを目的として研究活動を行う。そのため、総括班(本研究課題)では領域の15名の計画研究代表者とおよそ20名の公募研究代表者が自由な発想のもと調和の取れた活動を展開できるよう総括と評価等を行う。具体的には、1.総括班会議の開催、2.公開シンポジウムの開催、3.若手研究者の育成、4.研究活動の広報、5.果の取りまとめ等を活動目的とし、領域全体の運営を執り行う。採択時の審査において、「リプログラミング等進歩の激しいこの分野でさらなるブレークスルー的な成果が生まれることを期待する」および「本領域の趣旨を生かした成果を期待する」との意見をいただいたので、これらの点を班員に徹底する。

Research Products

• [Journal Article] Proviral silencing in embryonic stem cells requires the histone methyltransferase ESET. (2010)
  • Author(s): Matsui, T., Leung, D., Miyashita, H., Maksakova, I.A., Miyachi, H., Kimura, H., Tachibana, M., Lorincz, M.C., Shinkai, Y.
  • Journal Title: Nature 464 Pages: 927-931
• [Journal Article] Transmission distortion by loss of p21 or p27 cyclin-dependent kinase inhibitors following competitive spermatogonial transplantation. (2010)
  • Author(s): Kanatsu-Shinohara, M., Takashima, S., Shinohara, T.
  • Journal Title: Proc. Natl. Acad. Sci. USA 107 Pages: 6210-6215
• [Journal Article] A signaling principle for the specification of the germ cell lineage in mice. (2009)
  • Author(s): Ohinata, Y., Ohta, H., Shigeta, M., Yamanaka, K., Wakayama, T., Saitou, M.
  • Journal Title: Cell 137 Pages: 398-400
• [Journal Article] Genetic reconstruction of mouse spermatogonial stem cell self-renewal in vitro by Ras-Cyclin D2 activation. (2009)
  • Author(s): Lee, J., Kanatsu-Shinohara, M., Morimoto, H., Kazuki, Y., Takashima, S., Oshimura, M., Toyokuni, S., Shinohara, T
  • Journal Title: Cell Stem Cell 5 Pages: 76-86
• [Journal Article] A regulatory circuit for piwi by the large Maf gene traffic jam in Drosophila. (2009)
  • Author(s): Saito, K., Inagaki, S., Mituyama, T., Kawamura, Y., Ono, Y., Sakota, E., Kotani, H., Asai, K., Siomi, H., Siomi. M.C.
  • Journal Title: Nature 461 Pages: 1296-1299
• [Journal Article] The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline. (2009)
  • Author(s): Shoji, M., Tanaka, T., Hosokawa, M., Reuter, M., Stark, A., Kato, Y., Kondoh, G., Okawa, K., Chujo, T., Suzuki, T., Hata, K., Martin, S.L., Noce, T., Kuramochi-Miyagawa, S., Nakano, T., Sasaki, H., Pillai, R.S., Nakatsuji, N., Chuma, S
  • Journal Title: Dev. Cell 17 Pages: 1-13
• [Journal Article] DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes. (2008)
  • Author(s): Kuramochi-Miyagawa, S., Watanabe, T., Gotoh, K., Totoki, Y., Toyoda, A., Ikawa, M., Asada, N., Kojima, K., Yamaguchi, Y., Ijiri, T.W., Hata, K., Li, E., Matsuda, Y., Kimura, T., Okabe, M., Sakaki, Y., Sasaki, H., Nakano, T.
  • Journal Title: Genes Dev. 22 Pages: 908-917
• [Journal Article] Endogenous siRNAs from naturally formed dsRNAs regulate transcripts in mouse oocytes. (2008)
  • Author(s): Watanabe, T., Totoki, Y., Toyoda, A., Kaneda, M., Kuramochi-Miyagawa, S., Obata, Y., Chiba, H., Kohara, Y., Kono, T., Nakano, T., Surani, M.A., Sasaki, Y., Sasaki, H.
  • Journal Title: Nature 453 Pages: 539-543
• [Journal Article] Maternal and zygotic Dnmt1 are necessary and sufficient for the maintenance of DNA methylation imprints during preimplantation development. (2008)
  • Author(s): Hirasawa, R., Chiba, H., Kaneda, M., Tajima, S., Li, E., Jaenisch, R., Sasaki, H.
  • Journal Title: Genes Dev. 22 Pages: 1607-1616
• [Journal Article] Complex genome-wide transcription dynamics orchestrated by Blimp1 for the specification of the germ cell lineage in mice. (2008)
  • Author(s): Kurimoto, K., Yabuta, Y., Ohinata, Y., Shigeta, M., Yamanaka, K., Saitou M.
  • Journal Title: Genes Dev. 22 Pages: 1617-1635
• [Journal Article] Critical function of Prdm14 for the establishment of the germ cell lineage in mice. (2008)
  • Author(s): Yamaji, M., Seki, Y., Kurimoto, K., Yabuta, Y., Yuasa, M., Shigeta, M, Yamanaka, K., Ohinata, Y., Saitou, M.
  • Journal Title: Nat. Genet. 40 Pages: 1016-1022
• [Journal Article] Homing of mouse spermatogonial stem cells to germline niche depends onβ1-integrin. (2008)
  • Author(s): Kanatsu-Shinohara, M., Takehashi, M., Takashima, S., Lee, J., Morimoto, H., Chuma, S., Raducanu, A., Nakatsuji, N., Fassler R., Shinohara, T.
  • Journal Title: Cell Stem Cell 3 Pages: 533-542
• [Remarks]
  • URL: http://www.brc.riken.go.jp/lab/mcd/germline/index.html