2022 Fiscal Year Final Research Report
Remodeling plant reproduction system by cell fate manipulations.
| Project Area | Remodeling Plant Reproduction System by Cell Fate Manipulations. |
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| Project/Area Number |
20H05778
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| Research Category |
Grant-in-Aid for Transformative Research Areas (B)
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| Allocation Type | Single-year Grants |
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| Review Section |
Transformative Research Areas, Section (III)
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| Research Institution | Yokohama City University |
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Principal Investigator |
Maruyama Daisuke 横浜市立大学, 木原生物学研究所, 准教授 (80724111)
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| Co-Investigator(Kenkyū-buntansha) |
山岡 尚平 京都大学, 生命科学研究科, 准教授 (00378770)
水多 陽子 名古屋大学, 高等研究院(WPI), 特任助教 (70645142)
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| Project Period (FY) |
2020-10-02 – 2023-03-31
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| Keywords | 配偶体 / 重複受精 / 精細胞 / 卵細胞 / 中央細胞 / シロイヌナズナ / ベンサミアナタバコ / ゼニゴケ |
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| Outline of Final Research Achievements |
In plant reproduction, haploid tissues or gametophytes produce gametes after rounds of cell/nuclear proliferation and differentiation of gamete progenitor cells. Interestingly, several evidences have supported the plasticity or flexibility of cell fate in male and female gametophytic cells. Recent studies have begun to identify key factors regulating the identities of gametophytic cells. In this research project, we examined the mechanisms of the determination or conversion of cell fates in reproductive cells, via ectopic expression or mutations of the key regulatory factors. As a result, we could show representatives of "Remodeling of plant reproduction", through a production of anuclear pollen tubes. Establishing new protocols of cell fate manipulation, as well as preparation of new and unique plant materials, our approach will become the first step to uncover the principles of reproduction and possibly contribute plant breeding in the future.
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| Free Research Field |
植物生殖学
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| Academic Significance and Societal Importance of the Research Achievements |
生殖という過程は有用な形質を持つ植物同士を掛け合わせて新たな形質をもつ新品種を生み出すための原動力といえる．被子植物の場合は，花粉管によって運ばれる同一の遺伝情報をもつ雌雄各2個の配偶子が融合する厳密な受精システムが存在し，その染色体の交換の様式も限定されている．本領域の唱える植物生殖改変では，多様な細胞構成の新規配偶体を組み込むことで，通常とは異なる生殖の経路を作出することが可能になる．本領域は遺伝子操作を通じて新規配偶体が作出可能であることを示す成果をあげた．同様のアプローチで今後，配偶体が本来持つ機能への理解が深まるとともに，有用な新規の生殖システム構築の基礎となることが期待される．
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