ゲノムアダプテーションのシステム的理解

2015 Fiscal Year Annual Research Report

• Project Area: Systematic study of chromosome adaptation
• Project/Area Number: 22125001
• Research Institution: Osaka University
• Principal Investigator: 篠原 彰 大阪大学, たんぱく質研究所, 教授 (00252578)
• Project Period (FY): 2010-04-01 – 2016-03-31
• Keywords: 染色体 / 染色体適応 / ゲノムアダプテーション / 減数分裂 / 生殖細胞

Outline of Annual Research Achievements

減数分裂では組換えにより、父親、母親由来のゲノム情報の混ぜ合せが起こるだけでなく、”新規のゲノム変動”が配偶子に生じ、それが次世代の表現型に影響を与えること（ゲノムアダプテーション）が近年知られつつある。このようなゲノムアダプテーションを介して、ゲノムの多様性が生物集団の中に生じ、長期的には新しいゲノムを生み出す言動力になっていると考えられている。本新学術領域はゲノムアダプテーションの分子レベルでの仕組みを明らかにすること、様々な生物で見られる特異的ゲノムアダプテーション現象を解析する、ゲノムアダプテーションを解析するツールを開発すること、を目的としていた。平成22年度に開始した本領域研究は２６年度が最終年度にあたる。本新学術領域の研究の進展を図ると共に、研究成果をまとめることが大切になる。特に、異分野の研究者が十分な連携の元で研究を推進した成果をまとめため、総括班が非常に重要な役割を担っている。過去５年間のまとめは、２６年度末に行った。さらに新学術のまとめを行うため、評価のための資料を作成し、ヒアリウングを受け、A-の評価を得た。また、最終国際シンポジウムとして、平成２８年３月１日から３日まで、淡路島夢舞台国際会議場で、新しくできた新学術領域“染色体OS”(代表、東大白髭克彦)と合同で開催した。外国人招待講演者に加え、若手の１０名の発表を行った。

Research Progress Status

27年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

27年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] New York University(米国)
  • Country Name: U.S.A.
  • Counterpart Institution: New York University
• [Int'l Joint Research] Chung-Ang University(韓国)
  • Country Name: KOREA (REP. OF KOREA)
  • Counterpart Institution: Chung-Ang University
• [Int'l Joint Research] IISER, Thiruvananthapuram(India)
  • Country Name: India
  • Counterpart Institution: IISER, Thiruvananthapuram
• [Int'l Joint Research] FMI for Biomedical Research(Switzerland)
  • Country Name: Switzerland
  • Counterpart Institution: FMI for Biomedical Research
• [Journal Article] The Paf1 Complex Shapes the Landscape of Double-strand Breaks along Meiotic Chromosomes in Saccharomyces cerevisiae (2016)
  • Author(s): Santosh, G. K., Patel, K.J., Colletti M.M., Sasanuma, H., Shinohara, M., Hochwagen, A., and A. Shinohara
  • Journal Title: Genetics Volume: 202 Pages: 497-512
  • DOI: 10.1534/genetics. 115.177287
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Chromosome synapsis alleviates Mek1-dependent suppression of meiotic DNA repair. (2016)
  • Author(s): Subramanian, V.V., MacQueen, A. J., Vader, G., Shinohara, M., Borde, V., Shinohara, A. and A. Hochwagen
  • Journal Title: Current Biology Volume: 14 Pages: e1002369.
  • DOI: 10.1371/journal.pbio.1002369.
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Rad61/Wpl1(Wapl), a cohesin regulator, controls chromosome compaction during meiosis. (2016)
  • Author(s): Challa, KK., Lee, MS., Shinohara, M., Kim, K.m and A. Shinohara.
  • Journal Title: Nucleic Acids Research Volume: in press Pages: gkw034
  • DOI: 0.1093/nar/gkw034
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] DNA damage response clamp contributes to chromosomal assembly of ZMM-SIC pro-crossover factors during meiosis. (2015)
  • Author(s): Shinohara, M, Hayashihara, K., Grubb, J.T., Bishop, D.K., and A. Shinohara.
  • Journal Title: J. Cell Sci. Volume: 128 Pages: 1494-1506
  • DOI: 10.1242/jcs.161554
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] ATM and SIRT6/SNF2H mediate transient H2AX stabilization when DSBs form by blocking HUWE1 to allow efficient γH2AX foci formation. (2015)
  • Author(s): Atsumi, Y., Minakawa, Y., Ono, M., Dobashi, S., Snohe, K., Shinohara, A., Takeda, S., Takagi, M., Takamatsu, N., Nakagama, H., Teraoka, H., and K.-I. Yoshioka
  • Journal Title: Cell Reports Volume: 13 Pages: 2728-2740
  • DOI: 10.1016/j.celrep.2015.11.054.
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Prophase pathway for cleavage-independent removal of cohesin from chromosome arms during late prophase-I in meiosis. EMBO workshop on Meiosis (2016)
  • Author(s): Challa, K., Shinohara, M. A. Shinohara.
  • Organizer: International symposium on Chromosome OS
  • Place of Presentation: 淡路夢舞台国際会議場（兵庫県・淡路市）
  • Year and Date: 2016-03-01 – 2016-03-03
  • Int'l Joint Research / Invited
• [Presentation] Prophase pathway for cleavage-independent removal of cohesin from chromosome arms during late prophase-I in meiosis. EMBO workshop on Meiosis (2015)
  • Author(s): Challa, K., Shinohara, M. A. Shinohara.
  • Organizer: FAOBMB meeting
  • Place of Presentation: Hyderabad, India
  • Year and Date: 2015-11-26 – 2015-11-30
  • Int'l Joint Research / Invited
• [Presentation] Prophase pathway for cleavage-independent removal of cohesin from chromosome arms during late prophase-I in meiosis. EMBO workshop on Meiosis (2015)
  • Author(s): Challa, K., Shinohara, M. A. Shinohara.
  • Organizer: International RecA and chromosome dynamics meeting
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2015-09-16 – 2015-09-17
  • Int'l Joint Research / Invited
• [Presentation] Prophase pathway for cleavage-independent removal of cohesin from chromosome arms during late prophase-I in meiosis. EMBO workshop on Meiosis (2015)
  • Author(s): Challa, K., Shinohara, M. A. Shinohara.
  • Organizer: EMBO workshop on meiosis
  • Place of Presentation: Oxford, UK
  • Year and Date: 2015-09-01 – 2015-09-03
  • Int'l Joint Research
• [Presentation] Prophase pathway for cleavage-independent removal of cohesin from chromosome arms during late prophase-I in meiosis. EMBO workshop on Meiosis (2015)
  • Author(s): A. Shinohara.
  • Organizer: Meiosis meeting in Spain
  • Place of Presentation: Salamanca, Spain
  • Year and Date: 2015-06-04 – 2015-06-06
  • Int'l Joint Research
• [Remarks] 大阪大学蛋白質研究所ゲノム～染色体機能研究室ホームページ
  • URL: http://www.protein.osaka-u.ac.jp/genome/Shinohara-HP-index.html
• [Funded Workshop] International Symposium on Chromosome OS (2016)
  • Place of Presentation: 淡路島夢舞台国際会議場、兵庫県淡路市
  • Year and Date: 2016-03-01 – 2016-03-03