Analysis of repertoire dynamics of germ cell development

2023 Fiscal Year Final Research Report

• Project Area: Ensuring integrity in gametogenesis
• Project/Area Number: 18H05551
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: National Institute for Basic Biology
• Principal Investigator: Yoshida Shosei 基礎生物学研究所, 生殖細胞研究部門, 教授 (60294138)
• Co-Investigator(Kenkyū-buntansha): 中村 隼明 広島大学, 統合生命科学研究科(生), 准教授 (30613723)
• Project Period (FY): 2018-06-29 – 2023-03-31
• Keywords: 生殖細胞 / 細胞系譜 / マウス / ニワトリ / クローン動態 / バーコーディング

Outline of Final Research Achievements

Germ cells are the one cell lineage that creates the next generation through formation of sperm and oocytes. In this study, we analyzed the process by which mouse male germ cells are born in the early embryo, become spermatogonial stem cells and spermatozoa, and are transmitted to the next generation. We elucidated the clonal reduction that occurs in primordial germ cells during embryogenesis, subsequent process of sperm stem cell formation, clonal competition that occurs in sperm stem cell pool, and during transmission to the next generation. Furthermore, we developed culture methods for chick primordial germ cells, consolidating the foundation for germ cell research in birds, whose germ cell formation process differs from that of mammals.

Free Research Field

発生生物学、生殖細胞学

Academic Significance and Societal Importance of the Research Achievements

生殖細胞の系譜を明らかにすることで、エピゲノム状態や新規突然変異が次世代に伝わることで起こる先天性遺伝疾患の原因やゲノム進化に対する理解が深まると期待される。