Spectroscopic study of non-destructive ionization process for mass spectrometry

2009 Fiscal Year Self-evaluation Report

• Project Area: Molecular Science for Supra Functional Systems ? Development of Advanced Methods for Exploring Elementary Process
• Project/Area Number: 19056008
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Tokyo Metropolitan University
• Principal Investigator: FUJINO Tatsuya Tokyo Metropolitan University, 東京理工学研究科, 准教授 (20360638)
• Project Period (FY): 2007 – 2011
• Keywords: 質量分析法 / MALDI法 / プロトン移動 / 時間分解分光

Research Abstract

MALDI法は試料の分子構造を壊さず「一分子一ピーク」といった分子量による質量分析を可能にする手法であり、不安定で変質しやすい代謝物質等の網羅的解析を可能とする。また表面にマトリクス分子を展開させた手法(SALDI法)では、修飾表面上に化学吸着した試料のみをイオン化させる手法であるため、従来困難であった「質量分析による定量」への道を開く方法として期待される。しかしながら分析試料に用いるマトリックス分子の選定は一般に経験則により行われるため、本法の可能性が最大限に活用されているとは言い難く、従って微量かつ貴重な生体試料への応用は困難である場合が多い。本研究では分析対象試料に対し、非解離・高感度・高効率化が可能なマトリックス・修飾(形状・分子)表面を、分子科学論的な観点から予想し開発することを目的とする。

Research Products

• [Journal Article] Pronounced Selectivity in MALDI Mass Spectrometry with 2, 4, 6-Trihydroxyacetophenone on Zeolite Surface: Intensity Enhancement of Protonated Peptides and Suppression of Matrix-related Ions (2010)
  • Author(s): Y. Komori, H. Shima, *T. Fujino, J.N. Kondo, K. Hashimoto, T. Korenaga
  • Journal Title: J. Phys. Chem. C 114 Pages: 1593-1600
  • Peer Reviewed
• [Journal Article] Picosecond Time-resolved Fluorescence Study of Poly Vinyl Methyl Ether Aqueous Solution (2009)
  • Author(s): T. Sakamaki, *T. Fujino, H. Hosoi, T. Tahara, T. Korenaga
  • Journal Title: Chem. Phys. Lett. 468 Pages: 171-175
  • Peer Reviewed
• [Journal Article] In-cell Viscosity Measurement Using a Fluorescence Up-conversion Microscope (2008)
  • Author(s): T. Fujino, K. Hirota, K. Ohta, T. Tahara
  • Journal Title: Chem. Lett. 37 Pages: 1240-1241
  • Peer Reviewed
• [Journal Article] MALDI Mass Spectrometry with Cyclodextrin-Supported Matrix (2008)
  • Author(s): S. Yamaguchi, T. Fujita, *T. Fujino, T. Korenaga
  • Journal Title: Anal. Sci 24 Pages: 1497-1500
  • Peer Reviewed
• [Journal Article] Femtosecond/picosecond Time-resolved Fluorescence Study of Hydrophilic Polymer Fine Particles (2007)
  • Author(s): D. Nanjo, H. Hosio, *T. Fujino, T. Tahara, T. Korenaga
  • Journal Title: J. Phys. Chem. B 111 Pages: 2759-2764
  • Peer Reviewed
• [Presentation] Spectroscopic Study of non-destructive ionization process for mass spectrometry (2009)
  • Author(s): T. Fujino
  • Organizer: International Symposium on "Molecular Science for Supra Functional System"
  • Place of Presentation: O-okayama, Tokyo
  • Year and Date: 2009-06-04
• [Presentation] In-cell Viscosity Measurements by Femtosecond Fluorescence Up-conversion Microscopy (2008)
  • Author(s): T. Fujino, K. Hirota, K. Ohta, T. Tahara
  • Organizer: Focus On Microscopy 2008
  • Place of Presentation: Awaji Osaka, Japan
  • Year and Date: 2008-04-15
• [Presentation] Ultrafast Solvation Dynamics of Hydrophilic Polymer Fine Particles (2007)
  • Author(s): T. Fujino, H. Hosio, D. Nanjo, T. Tahara, T. Korenaga
  • Organizer: XXIII International Conference on Photochemistry
  • Place of Presentation: Koln Germany
  • Year and Date: 2007-08-02
• [Book] Ultrafast Fluorescence Microscope Chapter 3 (2009)
  • Author(s): T. Fujino, T. Tahara
  • Total Pages: 51-70
  • Publisher: CRC Press
• [Patent(Industrial Property Rights)] 包接マトリックスを用いたmaldi法
  • Inventor(s): 藤野竜也, 山口惣大, 伊永隆史
  • Industrial Property Rights Holder: 公立大学法人首都大学東京
  • Industrial Property Number: 特願2008-025881