Logistics for the regulation of higher neuronal function

2012 Fiscal Year Final Research Report

• Project Area: Intracellular logistics: interdisciplinary approaches to pathophysiology of membrane traffic
• Project/Area Number: 20113004
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokushima
• Principal Investigator: SASAKI Takuya 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (40241278)
• Project Period (FY): 2008 – 2012
• Keywords: 高次神経機能 / シナプス小胞 / シナプス形成 / 神経突起形成 / Rab13 / JRAB / アクチン細胞骨格 / ノックアウトマウス

Research Abstract

Rab family small G proteins serve as molecular switches in the regulation of vesicle transport. In this study, we focus on the role and mode of action of Rab3A, Rab13, and their related proteins in higher neuronal functions. As to Rab3A, we found using the knockout mice for Rabconnectin, the Rab3A-related protein, is involved in neuronal function, probably through formation of synaptic organization. We also found that the Rab13-JRAB system regulates the reorganization of actin cytoskeleton during neuronal extension. Our results suggest that this system may simultaneously coordinate vesicle transport and actin cytoskeletal reorganization in neurons.

Research Products

• [Journal Article] Rab13 small G protein and Junctional Rab13-binding protein (JRAB) orchestrate actin cytoskeletal organization during epithelial Junctional development (2012)
  • Author(s): Sakane, A., Abdallah, A.A.M., Nakano, K., Honda, K., Ikeda, W., Nishikawa, Y., Matsumoto, M., Matsushita, N., Kitamura. T., and Sasaki, T
  • Journal Title: J. Biol. Chem Volume: 287(51) Pages: 42455-424680
  • DOI: DOI:10.1074/jbc.M112.383653
  • Peer Reviewed
• [Journal Article] Expression of RASSF6 in kidney and the implication of RASSF6 and the Hippo pathway in the sorbitol-induced apoptosis in renal proximal tubular epithelial cells (2012)
  • Author(s): Withanage, K., Nakagawa, K., Ikeda, M., Kurihara, H., Kudo, T., Yang, Z., Sakane, A., Sasaki, T. and Hata, Y
  • Journal Title: J. Biochem Volume: 152(1) Pages: 111-119
  • DOI: DOI:10.1093/jb/mvs056
  • Peer Reviewed
• [Journal Article] Rab13 regulates neurite outgrowth in PC12 cells through its effector protein, JRAB/MICAL-L2 (2010)
  • Author(s): Sakane, A., Honda, K. and Sasaki, T
  • Journal Title: Mol. Cell. Biol Volume: 30(4) Pages: 1077-1087
  • DOI: DOI:10.1128/MCB.01067-09
  • Peer Reviewed
• [Journal Article] Rubicon and PLEKHM1 negatively regulate the endocytic/autophagic pathway via a novel Rab7-binding domain (2010)
  • Author(s): Tabata, K., Matsunaga, K., Sakane, A., Sasaki, T., Noda, T. and Yoshimori, T
  • Journal Title: Mol. Biol. Cell Volume: 21(23) Pages: 4162-4173
  • DOI: DOI:10.1091/mbc.E10-06-0495
  • Peer Reviewed
• [Journal Article] Rab family small G proteins in regulation of epithelial apical junctions (2009)
  • Author(s): Nishimura, N. and Sasaki, T
  • Journal Title: Front. Biosci Volume: 14 Pages: 2115-2129
  • DOI: http://www.ncbi.nlm.nih.gov/pubmed/19273188
• [Journal Article] APP anterograde transport requires Rab3A GTPase activity for assembly of the transport vesicle (2009)
  • Author(s): Szodorai, A., Kuan, Y-H., Hunzelmann, S., Engel, U., Sakane, A., Sasaki, T., Takai Y., Kirsch, J., Muller, U., Beyreuther, K., Brady, S., Morfini, G. and Kins, S
  • Journal Title: J. Neurosci Volume: 29 (46) Pages: 14534-14544
  • DOI: DOI:10.1523/ JNEUROSCI.1546-09.2009
  • Peer Reviewed
• [Journal Article] Doc2α and Munc13-4 regulate Ca^<2+>-dependent secretory lysosome exocytosis in mast cells (2008)
  • Author(s): Higashio, H., Nishimura, N., Ishizaki, H., Miyoshi, J., Orita, S., Sakane, A. and Sasaki, T
  • Journal Title: J. Immunol Volume: 180 (7) Pages: 4774-4784
  • DOI: http://www.ncbi.nlm.nih.gov/pubmed/18354201
  • Peer Reviewed
• [Journal Article] Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions (2008)
  • Author(s): Nakatsuji, H., Nishimura, N., Yamamura, R., Kanayama, H. and Sasaki, T
  • Journal Title: Mol. Cell. Biol Volume: 28 (10) Pages: 3324-3335
  • DOI: DOI:10.1128/MCB.00144-08
  • Peer Reviewed
• [Journal Article] Interaction of Rab3B with microtubule-binding protein Gas8 in NIH 3T3 cells (2008)
  • Author(s): Nishimura, N., Araki, K., Shinahara, W., Nakano, Y., Nishimura, K., Higashio, H. and Sasaki, T
  • Journal Title: Arch. Biochem. Biophys Volume: 474(1) Pages: 136-142
  • DOI: DOI:10.1016/j.abb.2008.03.032
  • Peer Reviewed
• [Journal Article] Cell-surface biotinylation to study endocytosis and recycling of occludin (2008)
  • Author(s): Nishimura, N. and Sasaki, T
  • Journal Title: Methods Mol. Biol Volume: 440 Pages: 89-96
  • DOI: DOI:10.1007/978-1-59745-178-9_7
• [Journal Article] Analysis on the emerging role of Rab3 GTPase-activating protein in Warburg Micro and Martsolf syndrome (2008)
  • Author(s): Sakane, A., Miyoshi, J., Takai, Y. and Sasaki, T
  • Journal Title: Methods Enzymol Volume: 438 Pages: 131-139
  • DOI: DOI:10.1016/S0076-6879(07)38009-9
• [Journal Article] Identification and characterization of JRAB/MICAL-L2, a junctional Rab 13-binding protein (2008)
  • Author(s): Nishimura, N. and Sasaki, T
  • Journal Title: Methods Enzymol Volume: 438 Pages: 141-153
  • DOI: DOI:10.1016/S0076-6879(07)38010-5
• [Presentation] 動く細胞 (2012)
  • Author(s): 佐々木卓也, 坂根亜由子
  • Organizer: ViEW2012 ビジョン技術の実利用ワークショップ
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2012-12-06
• [Presentation] 細胞接着・運動において Rab13-JRAB系が制御する小胞輸送とアクチン細胞骨格再編成 (2011)
  • Author(s): 佐々木卓也, 坂根亜由子
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(名古屋市)
  • Year and Date: 2011-10-03
• [Presentation] A role of JRAB in epithelial junctional development: The cross-talk between vesicular trafficking and actin cytoskeleton in the submembrane plaque, 細胞間接着形成過程においてJRAB が制御する細胞内小胞輸送とアクチン細胞骨格系 の細胞膜直下でのクロストーク機構 (2011)
  • Author(s): Ayuko Sakane and Takuya Sasaki
  • Organizer: 第63回日本細胞生物学会大会ミニシンポジウム
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2011-06-28
• [Presentation] Functions of Rab family small G proteins in neurite outgrowth. 神経突起形成における Rabファミリー低分子量G蛋白質の機能 (2010)
  • Author(s): Takuya Sasaki and Ayuko Sakane
  • Organizer: 第62回日本細胞生物学会大会シンポジウム
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 2010-05-20
• [Presentation] The Rubicon family negatively regulates the endocytic pathway through the interactions with Rab7, Rubiconファミリーは Rab7との結合を介してエンドサイトーシス経路を負に制御する (2010)
  • Author(s): Keisuke Tabata, Kohichi Matsunaga, Ayuko Sakane, Takuya Sasaki, Takeshi Noda and Tamotsu Yoshimori
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 2010-05-19