Development of functional Raman probes for innovative multiplex imaging

2022 Fiscal Year Final Research Report

• Project Area: Innovative multiplex imaging with functional Raman probes
• Project/Area Number: 20H05724
• Research Category: Grant-in-Aid for Transformative Research Areas (B)
• Allocation Type: Single-year Grants
• Review Section: Transformative Research Areas, Section (II)
• Research Institution: Tokyo Institute of Technology (2022); The University of Tokyo (2020-2021)
• Principal Investigator: Kamiya Mako 東京工業大学, 生命理工学院, 教授 (90596462)
• Co-Investigator(Kenkyū-buntansha): 小嶋 良輔 東京大学, 大学院医学系研究科(医学部), 助教 (10808059)
• Project Period (FY): 2020-10-02 – 2023-03-31
• Keywords: ラマンプローブ / 多重検出

Outline of Final Research Achievements

In this research project, we established the principle for controlling Raman signal based on the resonance Raman effect accompanied by changes in molecular absorption, and developed several functional Raman probes based on the principle. Specifically, we developed not only Raman probes whose Raman signals are activated upon reaction with enzymes such as aminopeptidases and glycosidases, but also Raman probes whose Raman signals are activated or switched in response to light irradiation. Furthermore, we applied the developed Raman probes to live cells and tissues to demonstrate the utility of these probes.

Free Research Field

ケミカルバイオロジー

Academic Significance and Societal Importance of the Research Achievements

本研究で確立した機能性ラマンプローブの分子設計をさらに拡張することで、生きた生物試料中における生体分子の機能や構造を従来法を凌駕する性能で可視化・検出するラマンイメージング技法が確立できると期待される。従って、生体解析ツールとしてのラマンイメージングの性能を飛躍的に拡張するとともに、医学・薬学・生物学に画期的な進展をもたらすと考えられ、それ故に学術的・社会的意義が極めて大きい成果といえる。