2013 Fiscal Year Final Research Report
Elucidating the central gamete fusion mechanism, conserved in plants and animals
| Project Area | Elucidation of Common Mechanisms for Allogeneic Authentication in Animals and Plants |
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| Project/Area Number |
21112008
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Waseda University (2011-2013)
The Institute of Physical and Chemical Research (2009-2010) |
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Principal Investigator |
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| Project Period (FY) |
2009-07-23 – 2014-03-31
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| Keywords | 受精 / 有性生殖 / 動植物共通機構 / 花粉 / GCS1 / GEX2 / 膜タンパク質 / 雄性配偶子 |
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| Research Abstract |
The aim of this study is elucidating the molecular mechanism of sperm-egg fusion. In the first half of the period, the analysis of functional domains in GCS1 protein, which is a gamete fusion factor conserved in plants, animals and protists, was performed. As a result, it was found that the functional GCS1 domain resides only in the N-terminus, and that the C-terminus following the transmembrane domain is dispensable for GCS1 function. Besides, in the last half, the functional analyses of GEX2, which was newly identified as a plant fertilization factor, was performed. As a result, it was found that Arabidopsis GEX2 mutant sperm cells could not be attached with female gametes, suggesting that GEX2 functions in gamete attachment for successful fertilization.
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[Journal Article] Sex-specific Post-translational Regulation of the Gamete Fusogen GCS1 in the Isogamous Volvocine Alga Gonium pectorale2014
Author(s)
Kawai-Toyooka, H., Mori, T., Hamaji, T., Suzuki, M., Olson, B.J., Uemura, T., Ueda, T., Nakano, A., Toyoda, A., Fujiyama, A., and Nozaki, H.
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Journal Title
Eukaryot. Cell. Eukaryot. Cell
Volume: 13(5)
Pages: 648-656
Peer Reviewed
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[Journal Article] Evidence for tubular mating structures induced in each mating type of heterothallic Gonium pectorale (Volvocales, Chlorophyta)2012
Author(s)
Mogi, Y., Hamaji, T., Suzuki, M., Ferris, P., Mori,T., Kabeya, Y., Miyagishima,S., and Nozaki, H.
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Journal Title
J. Phycol.
Volume: 48(3)
Pages: 670-674
Peer Reviewed
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[Journal Article] DNA packaging proteins Glom and Glom2 coordinately organize the mitochondrial nucleoid of Physarum polycephalum2011
Author(s)
Itoh, K., Izumi, A., Mori, T., Dohmae, N., Yui, R., Maeda-Sano, K., Shirai, Y., Kanaoka, M.M., Kuroiwa, T., Higashiyama, T., Sugita, M., Murakami-Murofushi, K., Kawano, S., and Sasaki, N
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Journal Title
Mitochondrion
Volume: 11(4)
Pages: 575-586
Peer Reviewed
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[Journal Article] Expression of the Cyanidioschyzon merolae stromal ascorbate peroxidase in Arabidopsis thaliana enhances thermotolerance2009
Author(s)
Hirooka, S., Misumi, O., Yoshida, M., Mori, T., Nishida, K., Yagisawa, F., Yoshida, Y., Fujiwara, T., Kuroiwa, H., and Kuroiwa, T.
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Journal Title
Plant Cell Rep
Volume: 28(12)
Pages: 1881-1893
Peer Reviewed
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[Journal Article] New protein Pmn34 with an exonuclease motif localizes in the mitochondrial nucleoid periphery of Physarum polycephalum2009
Author(s)
Itoh, K., Izumi, A., Mori, T., Dohmae, N., Yui, R., Sano, K., Kanaoka, M.M., Kuroiwa, H., Kuroiwa, T., Higashiyama, T., Murakami-Murofushi, K., Kawano, S., and Sasaki, N.
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Journal Title
Cytologia
Volume: 74(4)
Pages: 401-407
Peer Reviewed
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[Journal Article] The PLASTID DIVISION1 and 2 components of the chloroplast division machinery determine the rate of chloroplast division in land plant cell differentiation2009
Author(s)
Okazaki, K., Kabeya, Y., Suzuki, K., Mori, T., Ichikawa, T., Matsui, M., Nakanishi, H., and Miyagishima, S.Y.
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Journal Title
Plant Cell
Volume: 21(6)
Pages: 1769-1780
Peer Reviewed
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