2014 Fiscal Year Final Research Report
Structural analysis of HLA class I/II molecules
Project Area | HLA polymorphism, disease and evolution |
Project/Area Number |
22133004
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Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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Allocation Type | Single-year Grants |
Review Section |
Biological Sciences
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Research Institution | The Institute of Physical and Chemical Research |
Principal Investigator |
YOKOYAMA Shigeyuki 独立行政法人理化学研究所, 横山構造生物学研究室, 上席研究員 (00159229)
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Co-Investigator(Kenkyū-buntansha) |
SHIROUZU Mikako 独立行政法人理化学研究所, ライフサイエンス技術基盤研究センター, 副センター長 (70280732)
HONMA Teruki 独立行政法人理化学研究所, ライフサイエンス技術基盤研究センター, チームリーダー (10466039)
|
Project Period (FY) |
2010-04-01 – 2015-03-31
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Keywords | HLA / ペプチド / 立体構造 / タンパク質 / 免疫学 / 医薬品設計 / X線結晶構造解析 / 構造生物学 |
Outline of Final Research Achievements |
In this project, we performed the protein expression, purification, and crystallization of HLA molecules, HLA-DP5, HLA-DR53, and HLA-DR4, bound to their antigenic peptide. Thereby, we successfully determined the crystal structure of HLA-DP5 in complex with Cryptomeria japonica 1 peptide (pCry j 1), derived from Japanese cedar pollen. Based on this structural information, we designed ten compounds to inhibit pCry j 1 binding to HLA-DP5, as evaluation test candidates for the peptide-binding affinity. Furthermore, we determined the crystal structure of HLA-DP5 in complex with pCry j 1, including the flanking region.
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Free Research Field |
構造生物化学
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