Ultra-High Speed Measurement of Cell Properties

2015 Fiscal Year Final Research Report

• Project Area: Hyper Bio Assembler for 3D Celluler Innovation
• Project/Area Number: 23106003
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Osaka University
• Principal Investigator: KANEKO MAKOTO 大阪大学, 工学(系)研究科(研究院), 教授 (70224607)
• Co-Investigator(Kenkyū-buntansha): HIGASHIMORI Mitsuru 大阪大学, 大学院工学研究科, 准教授 (30346522) ; TADAKUMA Kenjiro 大阪大学, 大学院工学研究科, 助教 (30508833)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Keywords: 高速ビジョン / 高速アクチュエータ / 高速細胞マニピュレーション / マイクロ流体チップ / 細胞ストレス試験

Outline of Final Research Achievements

In order to extract the pure deformability characteristics of cells, it is necessary to improve the resolution of cell actuation in the sensing system. This work discussed how to achieve a high speed cell manipulation with high resolution under a micro fluidic chip. A novel concept in this work is the flow reduction mechanism, where a flow is generated by a macro actuator placed outside of a micro fluidic chip. The flow can be drastically reduced at the cell manipulation point in a micro channel due to the elasticity embedded into the micro fluidic chip. By using this advantage, we could construct a high resolution cell manipulation system and successfully achieved the cell positioning based on a visual feedback control with a resolution of 240nm, together with the maximum frequency of 130Hz.
As an application example, we showed a new concept of cell fatigue evaluation where we continuously impart mechanical stress to the cell until it eventually get a damage.

Free Research Field

メカトロニクス