Organ malformations as results of accumulated polarity disruptions in epithelial tubular structures during organogenesis and histogenesis

2015 Fiscal Year Final Research Report

• Project Area: Regulation of polarity signaling during morphogenesis, remodeling, and breakdown of epithelial tubular structure
• Project/Area Number: 23112006
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Shimane University
• Principal Investigator: Otani Hiroki 島根大学, 医学部, 教授 (20160533)
• Co-Investigator(Kenkyū-buntansha): HATTA Toshihisa 金沢医科大学, 医学部, 教授 (20238025) ; UDAGAWA Jun 滋賀医科大学, 医学部, 教授 (10284027)
• Research Collaborator: HASHIMOTO Ryuju ; NAITO Kanta ; MATSUMOTO Akihiro ; FURUYA Motohide ; OGAWA Noriko ; INOUE Takayuki ; RAFIQ Ashiq Mahmood ; JAHAN Esrat ; MOTOYA Tomoyuki ; NITTA Tetsuya ; KURAMOTO Junko ; HIRANO Satoru ; ZHANG Qinghua ; LIANG Shuai ; DEREJE Getachew Regassa ; KANEDA Ryo ; SAEKI Yuko ; TAKEDA Yumiko
• Project Period (FY): 2011-04-01 – 2016-03-31
• Keywords: 上皮管腔組織 / 極性 / 器官形成 / 組織形成 / 形態学 / 数理解析

Outline of Final Research Achievements

Basic morphological data on developing epithelial tubular structures were obtained applying mathematical methods as standards to evaluate abnormal development, and findings including those in Ror2 knockout mice were reported. Ｗe proved interkinetic nuclear migration (INM), a regulatory mechanism of stem cell proliferation/differentiation reported in the neural epithelium, in the developing epithelium of multiple organs of three germ-layer origins. We showed that INM is a general strategy for the regulated proliferation/differentiation of progenitor cells in the epithelial tubular structures, and also suggested a link between INM defects and malformation of organs.
We improved the exo-utero development method, an experimental analysis of organ histogenesis, and published the report and review papers. We also developed a kit for producing cleared biological specimens and method for producing cleared biological specimens for fine and effective 3-D observation.

Free Research Field

発生生物学、先天異常学、解剖学