Development of a large scale analysis platform for ncRNA neotaxonomy

2018 Fiscal Year Final Research Report

• Project Area: Neo-taxonomy of noncoding RNAs
• Project/Area Number: 26113008
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: ASAHARA Hiroshi 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (70294460)
• Research Collaborator: CHIBA Tomoki ; MATSUSHIMA Takahide ; KURIMOTO Ryota ; KATAOKA Kensuke ; YANO Yuki ; UCHIDA Yutaro ; ITO Yoshiaki
• Project Period (FY): 2014-07-10 – 2019-03-31
• Keywords: ノンコーディングRNA / マイクロRNA / 関節炎 / 遺伝子編集 / 細胞内構造体

Outline of Final Research Achievements

We developed and introduced new technologies and strategies such as high-content microscopy systems, gene libraries, genome editing technologies to extend known ncRNA taxon, and further complex that each ncRNA taxon makes We aimed to identify and analyze their behavior, and search for new ncRNA taxons. In addition, we have optimized the genome editing technology for functional analysis of ncRNA, and developed a method to analyze the function of newly identified ncRNA at high speed and genetically, accelerating research on ncRNA neotaxonomy.

Free Research Field

システム医学

Academic Significance and Societal Importance of the Research Achievements

本研究においては、ハイコンテンツ顕微鏡、GFPタグ遺伝子ライブラリー、遺伝子編集での個体作成などの新しい技術、戦略を開発あるいは導入し、これらシステムを複合的に用いることで、新規ノンコ―ディングRNAの同定とその機能解析による分類を細胞・個体レベルで進めることができた。結果、関節炎、骨系統疾患や白血病の病態解明や治療法の開発に貢献することができた。