• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1992 Fiscal Year Final Research Report Summary

Physiological roles of tachykinin peptides and their receptor subtypes

Research Project

Project/Area Number 02454140
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionFukuoka University

Principal Investigator

KAMIYA Hiro-o  Fukuoka Univ., Dept. of Pharmacol., Prof., 薬学部, 教授 (90028366)

Co-Investigator(Kenkyū-buntansha) HONDA Kenji  Fukuoka Univ., Dept. of Pharmacol., Assistant Prof., 薬学部, 助手 (60140761)
SAITO Ryo  Fukuoka Univ., Dept. of Pharmacol., Assistant Prof., 薬学部, 助手 (80122696)
TAKANO Yukio  Fukuoka Univ., Dept. of Pharmacol., Assistant Prof., 薬学部, 助手 (50113246)
Project Period (FY) 1990 – 1992
Keywordssubstance P / tachykinins / cardiovascular regulation / vasopressin / NK-1 receptor / blood pressure / hypothalamus
Research Abstract

1. Central cardiovascular regulation of tachykinin peptides.
The central pressor responses due to substance P (SP), neurokinin A (NKA) and neuropeptidegamma (NPgamma) were blocked by sympathetic blocking agents. These results suggest that central SP, NKA and NPgamma, derived from the preprotachykinin A gene, increase the blood pressure (BP) and heart rate via sympathetic nerve activity. In contrast, the pressor response to an neurokinin B (NKB) analogue senktide was inhibited by a vasopressin antagonist, and senktide caused an increase in plasma vasopressin level. In addition, injection of senktide into the paraventricularnucleus (PVN) of the hypothalamus also increased the BP. These results suggest that central NKB, derived from the preprotachykinin B gene, stimulated the NK-3 receptor in the PVN, and increase the BP via release of vasopressin from the pituitary gland.
2. Tachykinin receptor subtype in the endothelial cell and vasodilation.
Agonists for the NK-1 tachykinin receptor elicited the potent, transient and endothelium-dependent relaxation. SP-induced relaxation and increase of cGMP content were inhibited by hemoglobin, methylene blue,L-NG-monomethyl-D-arginine. These results suggest that the relaxation induced by SP is mediated by endothelium-derived relaxingfactor (EDRF). In addition, we examined ^<125>I-SP binding to the endothelial cell membranes of porcine aorta.The cells had a single high affinity binding site with Kd=0.10 nM and Bmax=52.2 fmol/mg protein.

  • Research Products

    (41 results)

All Other

All Publications (41 results)

  • [Publications] Takano,Y.: "Role of central tachykinin peptides in cardiovascular regulation in rats." Brain Res.528. 231-237 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saito,R.: "Characterization of tachykinin receptors in endothelial cells of porcine atrery." Neurosci.Lett.110. 337-342 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsumoto,H.: "Structure-activity relationships of dimeric analogs of tachykinin peptides:Correlation between spacer length and biological activities." Peptide Chemistry. 1989. 185-188 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 神谷 大雄: "タキキニン受容体の細分化" 医学のあゆみ. 152. 91- (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hagio,T.: "The central pressor actions of a novel tachykinin peptide,γ-preprotachykinin-(72-92)-peptide amide(NPg):Involvement of the sympathetic nervous system." Eur.J.Pharmacol.192. 173-176 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Murozono,T.: "Synthesis and biological activity of neurokinin A analogs containing S-activated cysteine residue at N-terminal portion." Peptide Chemistry. 1990. 265-268 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takano,Y.: "Role of NK-1 receptor in central cardiovascular regulation in rats:Studies on a novel non-peptide antagonist,CP-96,345 of substance P NK-1 receptor." Neurosci.Lett.139. 122-125 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakayama,Y.: "Central pressor actions of tachykinin NK-3 receptor in the paraventricular nucleus of the rat hypothalamus." Brain Res.595. 339-342 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Murozono,T.: "Neurokinin A analogs containing S-activated cysteine for affinity labelling of neurokinin-2 receptor." Peptide Chemistry. 1991. 275-278 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsumoto,H.: "Optimum cross-linking spacer length of dimeric neurokinin B analogs for interaction with NK-1 tachykinin receptors." Bull.Chem.Soc.Jpn.66. 1-9 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takano,Y.: "The mechnism of central pressor actions of tachykinin NK-3 receptor in the paraventricular nucleus of the hypothalamus in rats." Reg.Peptide.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saigo,H.: "Role of NK-1 receptor in central cardiovascular regulation in rast:studies on a novel non-peptide antagonist,CP-96,345,of substance P NK-1 receptor." Reg.Peptide.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matumoto,H.: "Contractile activity of Trimeresurus flavoviridis phospholipase A_2 on guinea pig ileum and artery." Biochemistry Intermational. 24. 181-186 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Honda,K.: "Relationship between substance P-induced initial transient contractions and inositol phospholipid hydrolysis in guinea pig ileum." J.Pharmacobio-Dyn.14. 393-397 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takano,Y.: "Tachykinin receptor subtypes:Cardiovasculas roles of tachykinin peptides." Asia Pacific Journal Pharmacol.6. 341-348 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 高野 行夫: "脳タキキニン・ペプチド受容体サブタイプと循環調節:新しいタキキニン・ペプチドneuropeptide γについて" 神経化学. 29. 400-401 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 高野 行夫: "タキキニン・ペプチド受容体と心血管作用機序" 最新医学. 46. 142-149 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 斎藤 亮: "タキキニン・ペプチドによる内皮細胞依存性血管弛緩作用" 福岡大学薬学紀要. 16. 1-12 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kamiya,H.: "Neuroreceptor Mechanisms in Brain(Eds.Kito,S.et al.)Plenim Press,New York" Evidence for cardiovascular roles of tachykinin peptides in the brain of the rat., 27-37 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takano,Y.: "Ann.N.Y.Acad.Sci.632" Tachykinin receptor sibtype:Central cardiovascular regulation of tachykinin peptides., 476-478 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saito,R.: "Ann.N.Y.Acad.Sci.632" Pharmacological properties of the tachykinin receptor sibtype in the endotherial cell and vasodilation., 457-459 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takano, Y.: "Role of central tachykinin peptides in cardiovascular regulation in rats." Brain Res.528. 231-237 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saito, R.: "Characterization of tachykinin receptors in endothelial cells of porcine artery." Neurosci. Lett.110. 337-342 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsumoto, H.: "Structure-activity relationships of dimeric analogs of tachykinin peptides: Correlation between spacer length and biological activities." Peptide Chemistry. 1989. 185-188 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kamiya, H. and Takano, Y.: "Tachykinin receptor subtypes (in Japanese)" Igakuno-Ayumi. 152. 91 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hagio, T.: "The central pressor actions of a novel tachykinin peptide, gamma-preprotachykinin-(72-92)-peptide amide (NPgamma): Involvement of the sympathetic nervous system." European J. Pharmacol.192. 173-176 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Murozono, T.: "Synthesis and biological activity of neurokinin A analogs containing S-activated cysteine residue at N-terminal portion." Peptide Chemistry. 1990. 265-268 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsumoto, H.: "Contractile activity of Trimeresurus flavoviridis phospholipase A2 on guinea pig ileum and artery." Biochemistry International. 24. 181-186 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Honda, K.: "Relationship between substance P-induced initial transient contractions and inositol phospholipid hydrolysis in guinea pig ileum." J. Pharmacobio-Dyn.14. 393-397 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takano, Y. and Kamiya, H.: "Tachykinin receptor subtypes: Cardiovascular roles of tachykinin peptides." Asia Pacific J. Pharmacol.6. 341-348 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takano, Y. and Kamiya, H.: "Tachykinin Receptor Subtypes; Cardiovascular roles of tachykinin peptides. (in Japanese)" The Saishin-Igaku. 46. 142-149 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saito, R.: "Endothelium-dependent vasodilation induced by tachykinin peptide. (in Japanese)" Pharmaceutical Bulletin of Fukuoka Univ. 16. 1-12 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takano, Y.: "Role of NK-1 receptor in central cardiovascular regulation in rats: Studies on a novel non-peptide antagonist, CP-96,345 of substance P NK-1 receptor." Neurosci. Lett.139. 122-125 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakayama, Y.: "Central pressor actions of tachykinin NK-3 receptor in the paraventricular nucleus of the rathypothalamus." Brain Res.595. 339-342 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Murozono, T.: "Neurokinin A analogs containing S-activated cysteine for affinity labelling of neurokinin-2 receptor." Peptide Chemistry. 1991. 275-278 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsumoto, H.: "Optimum cross-linking spacer length of dimeric neurokininB analogs for interaction with NK-1 tachykinin receptors." Bull. Chem. Soc. Jpn.66. 1-9 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kamiya, H.: "Evidence for cardiovascular roles of tachykinin peptides in the brain of the rat." Neuroreceptor Mechanisms in Brain (Eds. Kito, S. et al.)Plenum Press, New York. 27-37 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takano, Y.: "Tachykinin receptor subtype: Central cardiovascular regulation of tachykinin peptides." Ann. N.Y. Acad. Sci.632. 476-478 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saito, R.: "Pharmacological properties of the tachykinin receptor subtype in the endotherial cell and vasodilation." Ann. N.Y. Acad. Sci.632. 457-459 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takano, Y.: "The mechanism of central pressor actions of tachykinin NK-3 receptor in the paraventricular nucleus of the hypothalamus in rats." Reg. Peptide. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saigo, A.: "Role of NK-1 receptor in central cardiovascular regulation in rats : studies on a novel non-peptide antagonist, CP-96,345, of substance P NK-1 receptor." Reg. Peptide. (1993)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1994-03-24  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi