1996 Fiscal Year Final Research Report Summary
Pathogenesis of HTLV-I positive polymyositis
| Project/Area Number |
07670721
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Kagoshima University |
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Principal Investigator |
HIGUCHI Itsuro University Hospital Kagoshima University Assistant Professor, 医学部・附属病院, 講師 (80183573)
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| Co-Investigator(Kenkyū-buntansha) |
IZUMO Shuji Faculty of Medicine Kagoshima University Professor, 医学部, 教授 (30143811)
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| Project Period (FY) |
1995 – 1996
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| Keywords | HTLV-I / Polymyositis / PCR in situ hybridization / HAM |
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| Research Abstract |
Patients with polymyositis (PM) in Kagoshima, Japan exhibit seropositivity to human T-cell lymphotropic virus type I (HTLV-I), which is higher than in the general population. A direct HTLV-I infection of muscle fibers was previously reported by in situ hybridization (ISH) in a patient dually infected with HIV and HTLV-I.While using the same technique of ISH,we could not detect HTLV-I in patients with PM infected with HTLV-I alone. We obtained biopsied muscle specimens from 5 HTLV-I positive PM patients, 5 HTLV-I negative PM patients, an autopsied muscle specimen from a HAM patient, and a lymph node from a patient with adult T-cell leukemia (ATL) as a positive control. Employing in situ PCR,we found the HTLV-I provirus in some of the CD4-positive infiltrating cells in HTLV-I-positive PM but not within the muscle fibers. Among HTLV-I-positive PM,we performed double staining of immunohistochemistry for CD4 or macrophage and in situ PCR for HTLV-I provirus on the same sections to determine the exact cell types of HTLV-I containing cells. We found HTLV-I in CD4-positive cells but not in macrophages. This finding suggests that most of the HTLV-I containing CD4-positive cells are not macrophages but lymphocytes.
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