MECHANISM OF CAR CINOGENESIS IN THE BILIATY SYSTEM-MOLECULAR BIOLOGICAL ANALYSIS OF PANCREATICOBILIARY MALJUNCTION USING CDNA MICROARRAY

2002 Fiscal Year Final Research Report Summary

• Project/Area Number: 12671246
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: YOKOHAMA CITY UNIVERSITY, SCHOOL OF MECICINE
• Principal Investigator: SEKIDO Hitoshi YOKOHAMA CITY UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF SURGERY II, ASISTANT PROFESSOR, 医学部, 講師 (90187849)
• Co-Investigator(Kenkyū-buntansha): HAYASHIZAKI Yoshide RIKEN CENOMIC SCIENCES CENTER, GENOMIC EXPLORATION RESEARCH GROUP, PROJECT DIRECTOR, ゲノム科学総合研究センター, プロジェクトディレクター (70192705) ; ICHIKAWA Takashi YOKOHAMA CITY UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF SURGERY II, ASISTANT PROFESSOR, 医学部, 助手 (80275049) ; ICHIKAWA Yasushi YOKOHAMA CITY UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF SURGERY II, ASISTANT PROFESSOR, 医学部, 助手 (70254208) ; OKAZAKI asushi RIKEN GENOMIC SCIENCES CENTER, GENOMIC EXPLORATION RESEARCH GROUP, TEAM LEADER, ゲノム科学総合研究センター, チームリーダー (80280733)
• Project Period (FY): 2000 – 2002
• Keywords: Pancreaticobiliary maljunction / Micro array / cDNA / Carcinogenesis

Research Abstract

The mechanism of gastrointestinal carcinogenesis was comprehensively analyzed using cDNA micro array. Materials were the cancerous or non-cancerous mucosa at the gallbladders or common bile ducts taken from surgical specimen of the patients with pancreaticobiliary maljunction (PBMJ). RNAs were extracted from these samples. Because the quantity of the RNA was considerably small, amplification was performed according to the linear amplification method using Amino MEGA Script T7 kit. Applying this technique, the enough RNAs were obtained form 1.5 to 3 micrograms of total RNA. The linearity analysis between before and after the amplification was performed and well correlation was confirmed, correlation coefficient 0.7. Comprehensive analyses of gene expressions were performed using RNA samples extracted from the surgical specimens in the patients with PBMJ. The changes of gene expression patterns were comprehensively detected by cDNA micro array. Comparing these changes between colonic adenomas and the biliary mucosae in PBMJ, this study may contribute to elucidating the mechanism of gastrointestinal carcinogenesis.

Research Products

• [Publications] Ichikawa Y, et al.: "Telomerase Activity and Bcl-2 expression in the Gallbladder of Pancreaticobiliary Maljunction -A Preliminary Study"J Hepatobiliary Pancreat Surg. (in press). (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Koyanagi Y: "Pancreaticobiliary Maljunction"Igaku Tosho. 413 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ichikawa Y, Kamiyama M, Sekido H, Ishikawa T, Miura Y, Kamiya N, Morita T, Shimada T: "Telomerase activity and Bcl-s expression in the gallbladder of pancreaticobiliary maljunction-A preliminary study"J Hepatobiliary Pancreat Surg (in press). 10. (2003)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sekido H, Morita T, Matsuo K, Miura Y, Kunihiro O, Ichikawa Y, Endo I, Togo S, Shimada H,: "Follow-up After Biliary Reconstruction in Pancreaticobiliary Maljunction (PBMJ): Are There Any Differences Between in PBMJ And in Non-PBMJ?"5th World Congress of the International Hepato-Pancreato-Biliary Association, Tokyo. 2002.
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sekido H, Shimada H, Morita T, Matsuo K, Kunihiro O, Miura Y, Tanaka K, Ichikawa Y, Endo I, Togo S: "Indications for Surgery and Optimal Extent of Resection of the Bile Duct: surgical Treatment for Pancreaticobiliary Maljunction, in Pancreaticobiliary Maljunction"Pancreaticobiliary Maljunction, Igaku Tosho, Tokyo. 303-310 (2002)
  • Description: 「研究成果報告書概要(欧文)」より