Physical and/or Chemical Methods for the Removal of Virus from Transfusion Blood

2002 Fiscal Year Final Research Report Summary

• Project/Area Number: 12794016
• Research Category: Grant-in-Aid for University and Society Collaboration
• Allocation Type: Single-year Grants
• Research Field: Environmental pharmacy
• Research Institution: HOKKAIDO UNIVERSITY
• Principal Investigator: KAMO Naoki Hokkaido Univ. Grad. School of Pharmaceutical Sci., Prof., 大学院・薬学研究科, 教授 (10001976)
• Co-Investigator(Kenkyū-buntansha): AZUMA Hiroshi Hokkaido Red Cross Blood Center, Research Director, 研究部長 ; IKEDA Hisami Hokkaido Red Cross Blood Center, President, 所長 ; NARA Toshifumi Hokkaido Univ. Grad. School of Pharmaceutical Sci., Inst., 大学院・薬学研究科, 助手 (30241350) ; HIRAYAMA Junichi Hokkaido Red Cross Blood Center, Researcher, 研究部員 ; ABE Hideki Hokkaido Red Cross Blood Center, Research Section Chief, 研究部係長
• Project Period (FY): 2000 – 2002
• Keywords: parvovirus / UV-irradiation / UVC / Heat treatment of virus / S / D treatment / Hemoglobin / CFU / DNA damage

Research Abstract

Blood for transfusion is subject to rigorous inspections for not contaminating virus. However, there are some inevitable possibilities for the contamination because of 1) the necessity of time to produce antibodies against the infected virus (window period) and 2) the existence of unknown virus. Therefore the aim of this research is to develop method of the removal or inactivation of virus in the transfusion blood.
1) We concentrated on parvovirus B19, one of non-enveloped virus which is resistant to the solvent/detergent or heat treatment. In addition, infection of this virus sometimes gives sever illness. First of all, we developed the method testing the infectivity using CFU-E formation. Irradiation of UV-C (750 Jm^<-2>) deceased the infectivity by a factor of 3. The co-presence of rutin or catechins prevents the denaturation of factor VIII without any decrease in the effect against the virus.
2) The UV-C treatment was found to be very useful for the inactivation of virus containing hemoglobin solutions that will be applied to emergency patients who need a lot of blood.
3) We observed a good correlation between the inactivation of virus and the amounts of DNA damage.

Research Products

• [Publications] Y.Huang et al.: "Complete deoxygenation from a hemoglobin solution by an electrochemical method and heat treatment for virus inactivation"Biotechnol Prog. 18(1). 101-107 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Y.Naito et al.: "Virus removal from hemoglobin solution using Planova Membrane"J Artif Organs. 5(2). 141-145 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] J.Hirayama et al.: "Comparison of the effects of different antiviral treatments on the antioxidant systems of stroma-free hemoglobin"Photochem Photobiol. 74(3). 461-464 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] H.Sugawara et al.: "Inactivation of parvovirus B19 in coagulation factor concentrates by UVC radiation"Transfusion. 41(4). 456-469 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Y.Yamada-Ohnishi et al.: "Cell-associated virus infectivity of primary HIV-1 isolate can be eliminated by a filtration/methylene blue photoinactivation system"Transfusion. 40(12). 1542-1543 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] J.Hirayama et al.: "Photoinactivation of vesicular stomatitis virus with Fullerene conjugated with methoxy polyethylene glycol amine"Biol Pharm Bull. 22(10). 1106-1109 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Y.Huang, S.Takeoka, H,Sakai, H Abe, J.Hirayama, K.Ikebuchi, H.Ikeda, E.Tsuchida.: "Complete deoxygenation from a hemoglobin solution by an electrochemical method and heat treatment for virus inactivation"Biotechnol Prog. 18(1). 101-107 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Y.Naito, I.Fukutomi, Y.Masada, H.Sakai, S.Takeoka, E.Tsuchida, H.Abe, J.Hirayama, K.Ikebuchi, H.Ikeda.: "Virus removal from hemoglobin solution using Planova Membrane"J Artif Organs. 5(2). 141-145 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] J.Hirayama, H.Abe, N.Kamo, K.Ikebuchi, H.Ikeda.: "Comparison of the effects of different antiviral treatments on the antioxidant systems of stroma-free hemoglobin"Photochem Photobio1. 74(3). 461-464 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] H.Sugawara, R.Motokawa, H.Abe, M.Yamaguchi, Y.Yamada-Ohnishi, J.Hirayama, H. Sakata, S.Sato, N.Kamo, K.Ikebuchi, H.Ikeda.: "Inactivation of parvovirus B19 in coagulation factor concentrates by UVC radiation assessment by an in vitro infectivity assay using CFU-E derived from peripheral blood CD34+ cells"Transfusion. 41(4). 456-469 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Y.Yamada-Ohnishi, T.Owada, H.Abe, J.Hirayama, H.Ikeda, K.Ikebuchi.: "Cell-associated virus infectivity of primary HIV-1 isolate can be eliminated by a filtration/methylene blue photoinactivation system"Transfusion. 40(12). 1542-1543 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] J.Hirayama, H.Abe, N.Kamo, T.Shinbo, Y.Ohnishi-Yamada, S.Kurosawa, K.Ikebuchi, S.Sekiguchi.: "Photoinactivation of vesicular stomatitis virus with Fullerene conjugated with methoxy polyethylene glycol amine"Biol Pharm Bull. 22(10). 1106-1109 (1999)
  • Description: 「研究成果報告書概要(欧文)」より