2000 Fiscal Year Annual Research Report

樹状突起受容野の形成過程における放射状グリアの役割

Research Project

Project/Area Number	12877001
Research Institution	Hokkaido University
Principal Investigator	渡辺雅彦北海道大学, 大学院・医学研究科, 教授 (70210945)
Keywords	グルタミン酸受容体 / 小脳 / プルキンエ細胞 / 樹状突起 / バーグマングリア / 放射状グリア / シナプス形成 / マウス
Research Abstract	大脳皮質や小脳皮質では、ニューロンの樹状突起の主軸が軟膜方向へ伸展し、その周囲にシナプスを形成する末梢枝が多数派生するという共通した構築を示す。このような樹状突起構築の指向性がどのような細胞メカニズムにより形成されるのかを検討する目的で、発達段階の小脳に注目して分子形態学的解析を行った。プルキンエ細胞樹状突起をカルビンジン抗体を用いて、バーグマングリアの放射状突起をグルタミン酸トランスポーターGLAST抗体を用いて免疫組織化学を行った。なお、これらの分子マーカーの有用性は、以前申請者が見い出したものである(Shibata et al., 1997 ; Nakagawa et al., 1998)。これらの抗体による免疫組織化学の結果、分子層の最表層を伸長しつつあるプルキンエ細胞の樹状突起は、バーグマングリアの放射状突起に沿って軟膜方向へと向うことが明かとなった。さらに、電子顕微鏡を用いて詳細に構造的関係を精査したところ、両者の細胞突起より派生するフィロポディア同士が密接に接触している事実が判明した。これらの事実は、『ニューロン樹状突起受容野の形成において、放射状グリアによる認識/接着/誘導機構が重要な役割を果たしている』という申請者が提案する作業仮説の妥当性を、強く示唆しる。

Research Products
(11 results)

All Other

All Publications (11 results)

[Publications] Yamada, Keiko: "Dynamic transformation of Bergmann glial fibers proceeds in correlation with dendritic outgrowth and synapse formation of cerebellar Purkinje cells."J.Comp.Neurol.. 418. 106-120 (2000)
[Publications] Tanaka, Jun: "Gq protein α subunits Gαq and Gαl1 are localized at postsynaptic extra-junctional membrane of cereballar Purkinje cells and hippocampal pyramidal cells."Eur.J.Neurosci.. 12. 781-792 (2000)
[Publications] Minami, T.: "Characterization of nociceptin/orphanin FQ-induced pain responses in conscious mice : Neonatal capsaicin treatment and N-methyl-D-aspartate receptor GluRε subunit knochout mice."Neuroscience. 97. 133-142 (2000)
[Publications] Mori, Fumiaki: "Developmental changes in expression of the three ryanodine receptor mRNAs in the mouse brain."Neurosci.Lett.. 285. 57-60 (2000)
[Publications] Kakizawa, Sho: "Critical period for activity-dependent synapse elimination in developing cerebellum."J.Neurosci.. 20. 4954-4961 (2000)
[Publications] Miyazaki, Taisuke: "B2 exon splicing of nonmuscle myosin heavy chain IIB is differently regulated in developing and adult rat brain."Neurosci.Res.. 37. 299-306 (2000)
[Publications] Furuya, Shigeki: "L-serine and glycine serve as major astroglia-derived trophic factors for cerebellar Purkinje neurons."Proc.Natl.Acad.Sci.USA. 97. 11528-11533 (2000)
[Publications] Fukaya, Masahiro: "Improved immunohistochemical detection of postsynaptically-located PSD95/SAP90 protein family by protease section pretreatment. A study in the adult mouse brain."J.Comp.Neurol.. 426. 572-586 (2000)
[Publications] Nakamura, Kazuhiro: "Enhancement of hippocampal LTP, reference memory and sensorymotor gating in mutant mice lacking a telencephalon-specific cell adhesion molecule."Eur.J.Neurosci.. (印刷中). (2001)
[Publications] Hashimoto, Koichi: "Climbing fiber synapse elimination during postnatal cerebellar development requires signal transduction involving Gαq and phospholipase Cβ4."Progress in Brain Res.. 124. 31-48 (2000)
[Publications] 渡辺雅彦: "脳神経科学イラストレイテッド"森寿,真鍋俊也,渡辺雅彦,岡野栄之,宮川剛(羊土社). 365 (2000)

2000 Fiscal Year Annual Research Report

樹状突起受容野の形成過程における放射状グリアの役割

Principal Investigator

渡辺 雅彦 北海道大学, 大学院・医学研究科, 教授 (70210945)

Research Products

[Publications] Yamada, Keiko: "Dynamic transformation of Bergmann glial fibers proceeds in correlation with dendritic outgrowth and synapse formation of cerebellar Purkinje cells."J.Comp.Neurol.. 418. 106-120 (2000)

[Publications] Tanaka, Jun: "Gq protein α subunits Gαq and Gαl1 are localized at postsynaptic extra-junctional membrane of cereballar Purkinje cells and hippocampal pyramidal cells."Eur.J.Neurosci.. 12. 781-792 (2000)

[Publications] Minami, T.: "Characterization of nociceptin/orphanin FQ-induced pain responses in conscious mice : Neonatal capsaicin treatment and N-methyl-D-aspartate receptor GluRε subunit knochout mice."Neuroscience. 97. 133-142 (2000)

[Publications] Mori, Fumiaki: "Developmental changes in expression of the three ryanodine receptor mRNAs in the mouse brain."Neurosci.Lett.. 285. 57-60 (2000)

[Publications] Kakizawa, Sho: "Critical period for activity-dependent synapse elimination in developing cerebellum."J.Neurosci.. 20. 4954-4961 (2000)

[Publications] Miyazaki, Taisuke: "B2 exon splicing of nonmuscle myosin heavy chain IIB is differently regulated in developing and adult rat brain."Neurosci.Res.. 37. 299-306 (2000)

[Publications] Furuya, Shigeki: "L-serine and glycine serve as major astroglia-derived trophic factors for cerebellar Purkinje neurons."Proc.Natl.Acad.Sci.USA. 97. 11528-11533 (2000)

[Publications] Fukaya, Masahiro: "Improved immunohistochemical detection of postsynaptically-located PSD95/SAP90 protein family by protease section pretreatment. A study in the adult mouse brain."J.Comp.Neurol.. 426. 572-586 (2000)

[Publications] Nakamura, Kazuhiro: "Enhancement of hippocampal LTP, reference memory and sensorymotor gating in mutant mice lacking a telencephalon-specific cell adhesion molecule."Eur.J.Neurosci.. (印刷中). (2001)

[Publications] Hashimoto, Koichi: "Climbing fiber synapse elimination during postnatal cerebellar development requires signal transduction involving Gαq and phospholipase Cβ4."Progress in Brain Res.. 124. 31-48 (2000)

[Publications] 渡辺雅彦: "脳神経科学イラストレイテッド"森寿,真鍋俊也,渡辺雅彦,岡野栄之,宮川剛(羊土社). 365 (2000)

渡辺雅彦北海道大学, 大学院・医学研究科, 教授 (70210945)