2002 Fiscal Year Final Research Report Summary
Group recognition for Natural Toxic Compounds based on Their Toxicity and Its Contribution to Rapid Analysis
| Project/Area Number |
13640604
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
分離・精製・検出法
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
HOSOYA Ken Kyoto Inst. of Tech., Dept of Polymer Sci., Associate Professor, 繊維学部, 助教授 (00209248)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Fragment Imprinting Method / Artificial Receptor / Mycrocystins / Pre-treatment / HPLC / Molecular Imprinting / Toxicity Recognition / Nano-technology |
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| Research Abstract |
The following results have been obtained along 2 years' project.
1)Development of novel fragment imprinting method
For a naturally occurring toxic compound, Microcystin, the novel fragment imprinting method was applied using Adda functional group as pseudo-template molecule. Interestingly, even alkylbenzenes could be utilized as effective pseudo-template to Microcystins, in addition, relatively loose molecular recognition ability based on alkylbenzene imprinted polymer was found to realize effective group recognition of Microcystins. This recognition was found to be based on their toxicity recognition.
2)Group recognition based on toxicity recognition
In addition to the results described above, an imprinting for glutamic acid moiety was also quite effective for the group recognition. This was found to be based on interesting exclusion mechanism for other less important functional groups of Microcystins.
3)Pre-treatment of environmental water
the separation media obtained showed concentration effect of the target homologues, while other contaminants were easily excluded from the separation media. This is very important because much easier pre-treatment can be done using the obtained separation media.
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