2002 Fiscal Year Annual Research Report

小児期にみられる肝炎後再生不良性貧血における造血障害機序の研究

Research Project

Project/Area Number	13670792
Research Institution	Nagoya University
Principal Investigator	小島勢二名古屋大学, 大学院・医学系研究科, 教授 (20313992)
Co-Investigator(Kenkyū-buntansha)	木村宏名古屋大学, 医学部・附属病院, 講師 (30303621)
Keywords	肝炎 / 再生不良性貧血 / E型肝炎ウィルス / パルボB19ウィルス
Research Abstract	肝炎後再生不良性貧血(再不貧)に先行する肝炎の多くは激症型であり、その原因ウィルスの同定が試みられていきた。当科のこれまでの検討でもnon Anon BnonC型であり、新たに肝炎ウイルスと同定されたG型肝炎ウィルスやTTVウィルスの関与も否定された。本年度になってこれまで我が国では存在しないと考えられていたE型肝炎ウィルスが、一部の激症型肝炎の原因となりうることが判明した。E型肝炎の感染経路は経口感染であり、患得援護再不貧の患者の多くは輸血歴がないことから、原因ウィルスの候補となりうる。肝炎後再不貧(n=16)、特発性再不貧(n=7)、正常人コントロール(n=7)のHEVlgM抗体、HEVlgG抗体を検討した。あわせてパルボB19lgG抗体、B19lgM抗体についても検討した。それぞれの抗体の陽性率は、HEVlgM(肝炎後:0/16、特発性0/7、正常人0/7)、HEVlgG(肝炎後:0/16、特発性0/7、正常人0/7)、パルボB19lgM抗体(肝炎後:0/16、特発性2/7、正常人0/7),パルボB19lgG抗体(肝炎後:11/16、特発性6/7、正常人2/7)であった。今回の結果からE型肝炎ウィルスが肝炎後再不貧に先行する肝炎の原因ウィルスである可能性は否定できると考えられた。パルボB19については、肝炎後再不貧患者において抗体保有率は高いが特発性再不貧患者も同様であり。原因ウィルスとは考えにくい。肝炎後再不貧に先行する肝炎の原因は不明であり、さらなる検討が必要である。

Research Products

(11 results)

All Other

All Publications (11 results)

[Publications] Tanaka N, Kojima S, et al.: "Expression of tegument protein pp65 of human cytomegalovirus (CMV) and its application to the analysis of viral-specific cellular immunity in CMV-infected individuals"Arch Virol. 147. 2045-2417 (2002)
[Publications] Ihara M, Kojima S, et al.: "Monitoring of active HHV-6 infection in bone marrow transplant recipients by real time PCR ; comparison to detection of viral DNA in plasma by qualitative PCR"Microbiol Immunol.. 46. 701-705 (2002)
[Publications] Kondo Y, Kojima S, et al.: "Transient pancytopenia associated with parvovirus infection in a healthy child"Pediatr Int.. 44. 695-697 (2002)
[Publications] Kojima S, et al.: "Risk factors for evolution of acquired aplastic anemia into myelodysplastic syndrome and acute myeloid leukemia after immunosuppressive therapy in children"Blood. 100. 786-790 (2002)
[Publications] Kojima S, et al.: "Outcome of 154 patients with severe aplastic anemia who received transplants from unrelated donors : The Japan Marrow Donor Program"Blood. 100. 799-803 (2002)
[Publications] Ohga S, Kojima S, et al.: "Treatment response of childhood aplastic anemia with chromosomal aberrations at diagnosis"Br J Haematol. 118. 313-319 (2002)
[Publications] Kudo K, Kojima S, et al.: "Successful engraftment of unrelated donor stem cells in two children with congenital amegakaryocytic thrombocytopenia(CAMT)"J Pediatr Hematol Oncol. 24. 79-80 (2002)
[Publications] Yoshikawa T, Kojima S, et al.: "Human herpesvirus 6 viremia in bone marrow transplant recipients : clinical features and risk factors"J Infect Dis. 185. 847-853 (2002)
[Publications] Yoshimi A, Kojima S. et al.: "Epstein-Barr virus specific T cell cytotoxicity is mediated through the perforin pathway in patients with lymphoproliferative disorders after allogeneic bone marrow transplantation"Br J Haematol. 116. 710-715 (2002)
[Publications] Ohara A, Kojima S. et al.: "Evolution of myelodysplastic syndrome and acute myelogenous leukemia in children with hepatitis-associated aplastic anemia"Br J Haematol. 116. 999-1010 (2002)
[Publications] Manabe A, Kojima S, et al.: "Allogeneic hematopoietic stem cell trransplantaton for 27 children with juvenile myelomonocytic leukemia diagnosed based on the criteria of the international JMML working Group"Leukemia. 16. 645-649 (2002)

2002 Fiscal Year Annual Research Report

小児期にみられる肝炎後再生不良性貧血における造血障害機序の研究

Principal Investigator

小島 勢二 名古屋大学, 大学院・医学系研究科, 教授 (20313992)

Research Products

[Publications] Tanaka N, Kojima S, et al.: "Expression of tegument protein pp65 of human cytomegalovirus (CMV) and its application to the analysis of viral-specific cellular immunity in CMV-infected individuals"Arch Virol. 147. 2045-2417 (2002)

[Publications] Ihara M, Kojima S, et al.: "Monitoring of active HHV-6 infection in bone marrow transplant recipients by real time PCR ; comparison to detection of viral DNA in plasma by qualitative PCR"Microbiol Immunol.. 46. 701-705 (2002)

[Publications] Kondo Y, Kojima S, et al.: "Transient pancytopenia associated with parvovirus infection in a healthy child"Pediatr Int.. 44. 695-697 (2002)

[Publications] Kojima S, et al.: "Risk factors for evolution of acquired aplastic anemia into myelodysplastic syndrome and acute myeloid leukemia after immunosuppressive therapy in children"Blood. 100. 786-790 (2002)

[Publications] Kojima S, et al.: "Outcome of 154 patients with severe aplastic anemia who received transplants from unrelated donors : The Japan Marrow Donor Program"Blood. 100. 799-803 (2002)

[Publications] Ohga S, Kojima S, et al.: "Treatment response of childhood aplastic anemia with chromosomal aberrations at diagnosis"Br J Haematol. 118. 313-319 (2002)

[Publications] Kudo K, Kojima S, et al.: "Successful engraftment of unrelated donor stem cells in two children with congenital amegakaryocytic thrombocytopenia(CAMT)"J Pediatr Hematol Oncol. 24. 79-80 (2002)

[Publications] Yoshikawa T, Kojima S, et al.: "Human herpesvirus 6 viremia in bone marrow transplant recipients : clinical features and risk factors"J Infect Dis. 185. 847-853 (2002)

[Publications] Yoshimi A, Kojima S. et al.: "Epstein-Barr virus specific T cell cytotoxicity is mediated through the perforin pathway in patients with lymphoproliferative disorders after allogeneic bone marrow transplantation"Br J Haematol. 116. 710-715 (2002)

[Publications] Ohara A, Kojima S. et al.: "Evolution of myelodysplastic syndrome and acute myelogenous leukemia in children with hepatitis-associated aplastic anemia"Br J Haematol. 116. 999-1010 (2002)

[Publications] Manabe A, Kojima S, et al.: "Allogeneic hematopoietic stem cell trransplantaton for 27 children with juvenile myelomonocytic leukemia diagnosed based on the criteria of the international JMML working Group"Leukemia. 16. 645-649 (2002)

小島勢二名古屋大学, 大学院・医学系研究科, 教授 (20313992)