Identification of genes associated with relapse of prostate cancer and gene therapy

2002 Fiscal Year Final Research Report Summary

• Project/Area Number: 13671640
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Kanazawa university
• Principal Investigator: MIZOKAMI Atsushi University Hospital assistant, 医学部附属病院, 助手 (50248580)
• Co-Investigator(Kenkyū-buntansha): KASHIDA Kiyoshi Obstetrics and Gynecology assistant professor, 医学系研究科, 助教授 (70186667) ; NAMIKI Mikio Obstetrics and Gynecology professor, 医学系研究科, 教授 (70155985)
• Project Period (FY): 2001 – 2002
• Keywords: prostate cancer / androgen dependency / relapse / COX-2

Research Abstract

We inoculated LNCaP cells, androgen-sensitive human prostate cancer cells, onto 5-week aged scid mice subcutneously with 50 % matrigel. After tumor was increased visually, we castrated the mouse. First, tumor retarded for 2 weeks. Then it stated to enlarge. We sacrificed the mouse and took the tumor and subcultured. (LN-RECO). Next we inoculated the LN-RECO to the castrated mouse. Again we took the enlarged tumor and subcultured (LN-REC4).
We characterized LN-REC4 cells. Although LN-REC4 cells were stimulated by androgen in vitro, the growth speed of those cells in the castrated mouse was faster than LNCaP cells, suggesting that LN-REC4 seemed to be a good model that reflect the relapse of prostate cancer.
We also investigated the COX-2 gene since this gene seemed to be related with progression of prostate cancer. Overexpression of COX-2 gene in LNCaP cells induced cell proliferation in vitro and in vivo, and induced VEGF secretion.

Research Products

• [Publications] Fujita, H., Koshida, K., Keller, E.T., Takahashi, Y., Yoshimoto, T., Namiki, M., Mizokami, A: "Cyclooxygenase-2 promotes prostate cancer progression"The prostate. 53. 232-240 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Fujita, H., Koshida, K., Keller, E. T., Takahashi, Y., Yoshimito, T., Namiki, M., Mizokami, A: "Cyclooxygenase-2 promotes prostate cancer progression"The prostate. 53. 232-240 (2002)
  • Description: 「研究成果報告書概要(欧文)」より