2002 Fiscal Year Final Research Report Summary
PROMOTING EFFECTS OF JUVENILE ESTROGEN TREATMENT ON DEVELOPMENT OF AUTOIMMUNE PROSTATITIS IN NEONATALLY THYMECTOMIZED MICE
| Project/Area Number |
13671684
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | AICHI CANCER CENTER RESEARCH INSTITUTE |
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Principal Investigator |
HAYASHI Norio AICHI CANCER CENTER RESEACH INSTITUTE RESARCHER, 研究所, 研究員 (70198852)
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| Co-Investigator(Kenkyū-buntansha) |
TAGUCHI Osamu AICHI CANCER CENTER RESEACH INSTITUTE, DIVISION OF MOLECULAR PATHOLOGY SECTION HEAD, 分子病態学部, 室長 (00142167)
SUGIMURA Yoshiki AICHI CANCER CENTER RESEACH INSTITUTE RESEARCHER, 研究所, 研究員 (90179151)
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| Project Period (FY) |
2001 – 2002
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| Keywords | ESTROGEN / PROSTATE / MOUSE / THYMECTOMY / PROSTATITIS / AUTO IMMUNE / NEONATAL |
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| Research Abstract |
Neonatal treatment of C57BL/6 male mice with estradiol-17β (E_2) (8mg/kg body weight) induces prostate epithelial hyperplasia and/or dysplasia with inflammatory cell infiltration. Five-consecutive-treatment from the day of birth (E_2-0)induced lesions in all mice. When the treatment was started on day 7 after birth (E_2-7), similar epithelial abnormalities with a lesser exert of inflammatory cell infiltration were caused in about the half mice. Few prostatic lesions were found when E_2 injection was started on day 14 (E_2- 14). Autoimmune prostatitis developed spontaneously in approximately 35% of C57BL/6 mice after thymectomy (Tx) on day 3 (Tx-3) after birth. When Tx-3 mice received E_2 injections from day 7 (Tx-3 + E_2-7) or day 14 (Tx-3 + E_2-14) but not day 21 (Tx-3 + E_2-21), a high incidence (around 80%) of severe prostatitis developed. Injections of testosterone propionate (TP) were without effect, even when given at young age. Autoantibodies against prostate epithelial cells were detected in the sera of Tx-3 mice with prostatitis, irrespective of whether given E_2 injections but never neonatal E_2 exposure alone. Transplantation of newborn prostate under the kidney capsules of Tx-3 or Tx-3 + E_2-14 mice with prostatitis evoked intense inflammation around the grafts. Such an inflammatory reaction was never seen with transplants into the E_2-0 mice. Development of prostatitis in Tx-3 and Tx-3 + E_2-14 but not E_2-0 mice could be prevented by the intraperitoneal injection of spleen cells from normal male mice at day 7. We conclude that effector belongs to the CD4^+CD25^+ class of lymphocytes.
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[Publications] Ishii, K., Usui, S., Sugimura, Y., Yoshida, S., Hioki, T., Tatematsu, M., Yamamoto, H., Hirano, K.: "Aminopeptidase N regulated by zinc in human prostate participates in tumor cell invasion"Int J Cancer.. 92. 49-54 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Ishii, K., Usui, S., Sugimura, Y., Yamamoto, H., Yoshikawa, K., Hirano, K.: "Inhibition of aminopeptidase N (AP-N) and urokinase-type plasminogen activator (uPA) by zinc suppresses the invasion activity in human urological cancer cells"Biol Pharm Bull.. 24. 226-230 (2001)
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[Publications] Ishii, K., Usui, S., Sugimura, Y., Yamamoto, H., Yoshikawa, K., Hiran, K.: "Extract from Serenoa repens suppresses the invasion activity of human urological cancer cells by inhibiting urokinase-type plasminogen activator"Biol Pharm Bull. 24. 188-190 (2001)
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[Publications] Ishii, K., Usui, S., Yamamoto, H., Sugimura, Y, Tatematsu, M., Hirano, K.: "Decreases of metallothionein and aminopeptidase N in renal cancer tissues"J Biochem.. 129. 253-258 (2001)
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[Publications] Kontani, K., Taguchi, O., Narita, T., Izawa, M., Hiraiwa, N., Zenita, K., Takeuchi, T., Murai, H., Miura, S., Kannagi, R.: "Modulation of MUC1 mucin as an escape mechanism of breast cancer cells from autologous cytotoxic T-lymphocytes"Br. J. Cancer. 84. 1258-1264 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Hirose, F., Ohshima, N., Shiraki, M., Inoue, Y. H., Taguchi, O., Nishi, Y., Matsukage, A. and Yamaguchi, M.: "Ectopic expression of DREF induces DNA synthesis, apoptosis, and unusual morphogenesis in the drosophia eye imaginal disc : possible interaction with polycomb and trithorax group proteins"Mol. Cell. Biol.. 21. 7231-7242 (2001)
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[Publications] Inoue, T., Hioki, T., Hayashi, N., Takahashi, S., Shirai, T., Sugimura, Y.: "Preoperative predictors of cancerous involvement of the neurovascular bundles in patients with localized prostate cancer"Int J Urol.. 9. 47-53 (2002)
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[Publications] Takahashi, S., Suzuki, S., Inaguma, S., Cho, Y.M., Ikeda, Y., Hayashi, N., Inoue, T., Sugimura, Y., Nishiyama, N., Fujita T, Ushijima, T., Shirai, T.: "Down-regulation of Lsm1 is involved in human prostate cancer progression"Br J Cancer.. 86. 940-946 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Kontani, K., Taguchi, O., Ozaki, Y., Hanaoka, J., Tezuka, N., Sawai, S., Inoue, S., Fujino, S., Maeda, T., Itoh, Y., Ogasawara, K., Sato, H., Ohkubo, I., Kudo, T.: "Novel vaccination protocol consisting of injecting MUC1 DNA and non-primed dendritic cells at the same region greatly enhanced MUC1 -specific anti-tumor immunity in a murine model"Cancer Gene Ther.. 9. 330-337 (2002)
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[Publications] Tei, K., Kawakami-Kimura, N., Taguchi, O., Kumamoto, K., Higashiyama, S., Taniguchi, N., Toda, K., Kawata, R., Hisa, Y., and Kannagi, R.: "Roles of cell adhesion molecules in tumor angiogenesis induced by co-transplantation of cancer and endothelial cells to nude rats"Cancer Res.. 62. 6289-6296 (2002)
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