2002 Fiscal Year Final Research Report Summary
Detection of susceptible genes for Cedar Pollen Allergy using SNPs in HLA region
| Project/Area Number |
13671795
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Nara Medical University |
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Principal Investigator |
IDE Takeshi Nara Medical University, Medicine, Research Associate, 医学部, 助手 (20094550)
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| Co-Investigator(Kenkyū-buntansha) |
ISHITANI Akiko Nara Medical University, Medicine, Research Assistant Professor, 医学部, 講師 (40112544)
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| Project Period (FY) |
2001 – 2002
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| Keywords | SNPs / HLA / polymorphism / susceptible gene / KIR gene / Japanese cedar pollinosis / allergy |
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| Research Abstract |
1. DNA samples from 92 healthy Japanese and 92 Japanese cedar pollinosis patients were studied. The healthy controls used in this study did not have anti-cedar-pollen , anti-Dactylis pollen, anti-Artemisia pollen, nor anti-mite antibody. Cedar-pollinosis patients had only anti-cedar-pollen antibody.
2. In HLA region, 150 SNPs were tested. Twenty-three SNPs of them were not detected polymorphism, and 28 SNPs of them were detected polimorphism less than 10% showing the ethnic difference between Caucasian and Japanese. Another 99 SNPs showed polimorphism more than 10 %.
3. The SNPs datas were analyzed for the association of SNPs and cedar pollinosis. One region with strong association was found, and the chi-square value was 11, P value was 0.001. And Odds ratio was 5.
4. The DNA samples were also studied for the Killer-Immunoglobulin like Receptor (KIR) gene polymophism. In this point, no significant association of KIR gene polymorphism and cedar pollinosis was found.
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