2002 Fiscal Year Final Research Report Summary
Real-time analysis on activity-dependent glucose uptake by usiug brain slices
| Project/Area Number |
13680893
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
神経・脳内生理学
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| Research Institution | Akita University |
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Principal Investigator |
YAMADA Katsuya Akita University School of Medicine Research Associate, 医学部, 助手 (40241666)
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| Project Period (FY) |
2001 – 2002
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| Keywords | glucose / fluorescence / uptake / brain |
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| Research Abstract |
Energy supply in the brain solely depends on glucose. However, the relationship between brain activity and glucose supply has not been well understood. By using 2-NBDG as a fluorescent glucose tracer, we have for the first time visualized glucose uptake into a single, living mammalian cells (Yamada et al, J. Biol. Chem. 2000). Our aim in the present study was to visualize glucose uptake into neurons. In conclusion, neurons dissociated from ventromedial hypothalamus, substantia nigra pars reticulata, and visual cortex incorporated 2-NBDG and showed an increase in the fluorescence. 2-NBDG was applied to visual cortical slices while the underlying white matter was electrically stimulated. Changes in the fluorescence intensity were detected in the area above the stimulating electrodes. In vivo experiments were also conducted in the somatosensory barrel cortex while the whiskers were mechanically stimulated after injection of 2-NBDG into artery. 2-NBDG fluorescence was observed in the site where optical intrinsic signals were the highest, implying an activity-dependent glucose uptake although more detailed study should be conducted.
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[Publications] Yamada,K., Ji,J.-J., Yuan,H., Miki,T., Sato,S., Horimoto,N., Shimizui,T., Seino,S., Inagaki,N.: "Protective role of ATP-sensitive potassium channels in hypoxia-induced generalized seizure."Science. 292. 1543-1546 (2001)
Description
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