2002 Fiscal Year Final Research Report Summary
Identification of activating factors for signal transduction released during ischemia in heart
| Project/Area Number |
13832005
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Institution | Yamaguchi University |
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Principal Investigator |
MIZUKAMI Yoichi Yamaguchi University Center for Gene Research, Associate Professor, 遺伝子実験施設, 助教授 (80274158)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Sei Yamaguchi University, School of Medicine, Professor, 医学部, 教授 (80225515)
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| Project Period (FY) |
2001 – 2002
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| Keywords | heart / ischemia / signal transduction / seretion / protein ourification / mass spectrometry / kinase / charomatography |
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| Research Abstract |
We purify survival factors released from cadiomyocytes exposed to ischemia. The supernatant of 100 liter was collected from cultured cardiomyocytes, and concentrated by ammonium sulfate. The sample was separated by column chromatography, and tested survival activity for cardiomyocytes. Two proteins having survival activity were identified using mass spectrometry. The molecular masses of these proteins are 33 kDa and 41 kDa, respectively. These proteins induce serine phosphorylation of 30 kDa protein, and the purified proteins show survival activity. We speculate that these proteins play a role for understanding of ischemia in heart and for development of drug discovery.
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Research Products
(20 results)
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[Publications] Fujimoto, K., Mizukami Y., Kimura, M. Todoroki-Ikeda.N, Mogami, K., Kobayasbi, S., and Matsuzaki, M.: "Molecular cloning of rat transmembrane domain 40-kDa regulated in adipocytes and its expression in H9c2. Cells exposed to ischemic hypoxia and reoxygenation"Biochim. Biophys. Acta. (Gene Structure & Expression). 1518. 173-177 (2001)
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[Publications] Omura,M., Kobayashi,S., Mizukami,Y.,Mogami,K.,Todoroki-Ikeda,N.,Miyake,T., and Matsuzaki, M.: "Eicosapentaenoic acid (EPA) induces Ca2+-independent activation and translocation of endothelial nitric oxide synthase and endothelium-dependent relaxation"FEBS Lett.. 487. 361-366 (2001)
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[Publications] Kimua, M., Mizukami, Y., Miura, T., Fujimoto, K., Kobayashi, S., and Matsuzaki, M.: "G-protein-coupled receptor, GPR41, induces apoptosis via a p53/Bax pathway during ischemic hypoxia and reoxygenation"J. Biol. Chem. 276. 26453-26460 (2001)
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[Publications] Mizukami, Y., Okamura, Y., Miura, T., Kimura, M., Mogami, K., Todoroki-Ikeda, N., Kobayashi, S, and Matsuzaki, M.: "Phosphorylation of proteins and apoptosis induced by c-Jun N-terminal kinase activation in H202 stimulation"Biochim. Biophys. Acta. (Molecular Cell Research). 1540. 213-220 (2001)
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[Publications] Aki, T., Mizukami, Y., Oka, Y., Yamaguchi, K., Uemura, K., Fujimiya, T., and Yoshida, K.: "Phosphatidylinositol3-kinase accelerates necrotic cell death during hypoxia"Biochem. J.. 358. 481-487 (2001)
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[Publications] Shirao, S., Kashiwagi, S., Sato, S., Miwa, S., Nakao, F., Kurokawa, T., Todoroki-Ikeda, N., Mogami, K., Mizukami, Y., Kuriyama, S., Haze, K., Suzuki, M., Kobayashi, S.: "Sphingophosphorycholine is a novel messenger for Rho-kinase-mediated Ca2+sensitization in the bovine cerebral artery : unimportant role for protein kinase C"Circ. Res.. 91. 112-119 (2002)
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[Publications] Nakao,F., Kobayashi, S., Mogami, K., Mizukami. Y., Shirao, S., Miwa, S., Todoro ki-Ikeda, N., Ito, M., Matsuzaki, M.: "Involvement of Src Family Protein Tyrosine Kinases in Ca2+-Sensitization of Coronary Artery Contraction Mediated by a Sphingosylphosphorylcholine-Rho-Kinase Pathway"Circ. Res. 91. 953-960 (2002)
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