2013 Fiscal Year Annual Research Report
細胞分化のためのnon-coding RNA機能のゲノム全体における識別
| Project/Area Number |
13F03717
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
CARNINCI Piero 独立行政法人理化学研究所, ライフサイエンス技術基盤研究センター, 副センター長
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| Co-Investigator(Kenkyū-buntansha) |
BONETTI Alessandro 独立行政法人理化学研究所, ライフサイエンス技術基盤研究センター, 外国人特別研究員
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| Keywords | ncRNA / mES / iPS |
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| Research Abstract |
During the first year of my JSPS fellowship I have been able to successfully complete the first two aims (AIM 1 "A comprehensive map of the ncRNAs of iPS and MEF cells" and AIM 2 "Preparation of reagents to experimentally alter ncRNAs expression") of my proposed plan and I have already started aim 3 ("Induction of de-differentiation by ncRNAs to produce iPS cells").
Briefly, we have identified around 100 ncRNAs that are specifically expressed in mES stage and not present in differentiated cells. We have performed a 3'RACE analysis in order to detect the 3'end of each of these molecules. We then cloned each ncRNA in an expression vector that is suitable for overexpression experiments in differentiated cells in order to drive de-differentiation. I am currently screening the role of each ncRNA in inducing pluripotency first by introducing each of them singularly and then pooling them into groups. I expect to have significant results by this combinatorial approach by the beginning of the second year of my JSPS fellowship.
Furthermore I have started to investigate the role of such transcripts in the maintenance of pluripotency by overexpressing them in mES cells that have been deprived of LIF growth factor.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Everything is proceeding according to the schedule.
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| Strategy for Future Research Activity |
I will proceed according the remaining aims of my proposed plan. Therefore I'll complete aim 3 ("Induction of de-differentiation by ncRNAs to produce iPS cells") and aim 4 (Differentiation mediated by ncRNAs from iPS to differentiated state).
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