2004 Fiscal Year Final Research Report Summary
Chromatin Remodeling and Epigenetics
| Project/Area Number |
14208086
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
HIROSE Susumu National Institute of Genetics, Department of Developmental Genetics, Professor, 個体遺伝研究系, 教授 (90022730)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIOKA Kenichi National Institute of Genetics, Department of Developmental Biology, Assistant Professor, 個体遺伝研究系, 助手 (80370120)
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| Project Period (FY) |
2002 – 2005
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| Keywords | Chromatin Remodling / Epigenetics / Drosophila / GAGA factor / FACT / Brahma Complex / Position Effect Variegation / Supercoiling Factor |
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| Research Abstract |
1) We elucidated the mechanism underlying the site-specific chromatin remodeling around the GAGA factor-binding site. First, GAGA factor recruits FACT to its binding site. Then, FACT induces a conformational change in the neighboring nucleosomes, which in turn facilitates chromatin remodeling.
2) We identified subunits of the Brahma chromatin remodeling compex as the GAGA factor-associated proteins besides FACT, suggesting that Brahma complex is involved in the GAGA factor-FACT complex induced chromatin remodeling.
3) Genetic analysis revealed that the GAGA factor-FACT complex contributes to epigenetic maintenance of Hox gene expression.
4) Genetic and biochemical analyses demonstrated that the GAGA factor-FACT complex is involved in position effect variegation.
5) We found a crucial role of Drosophila supercoiling factor in the dosage compensation of X chromosome using RNAi in vivo.
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[Journal Article] Nucleosomal histone kinase-1 phosphorylates H2A Thr 119 during mitosis in the early Drosophila embryo.2004
Author(s)
Aihara H, Nakagawa T, Yasui K, Ohta T, Hirose S, Dhomae N, Takio K, Kaneko M, Takeshima Y, Muramatsu M, Ito T.
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Journal Title
Genes Dev. 18
Pages: 877-888
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification of the human IAI.3B promoter element and its use in the construction of a replication-selective adenovirus for ovarian cancer therapy.2003
Author(s)
Hamada, K., Kohno, S., Iwamoto, M., Yokota, H., Okada, M., Tagawa, M., Hirose, S., Yamasaki, K., Shirakata, Y., Hashimoto, K., Ito, M.
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Journal Title
Cancer Res. 63
Pages: 2506-2512
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Facile synthesis of site-specifically acetylated and methylated histone proteins: reagents for evaluation of the histone code hypothesis.2003
Author(s)
He, S., Bauman, D., Davis, J.S., Loyola, A., Nishioka, K., Gronlund, J.L., Reinberg, D., Meng, F., Kelleher, N., McCafferty, D.G.
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Journal Title
Proc. Natl. Acad. Sci. USA. 100
Pages: 12033-12038
Description
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[Journal Article] Tracking FACT and RNA polymerase II elongation complex. through chromatin in vivo.2003
Author(s)
Saunders, A., Werner, J., Andrulis, E.D., Nakayama, T., Hirose, S., Reinberg, D., Lis, J.T.
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Journal Title
Science 301
Pages: 1094-1096
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Drosophila FACT contributes to Hox gene expression through physical and functional interactions with GAGA factor.2003
Author(s)
Simojima, T., Okada, M., Nakayama, T., Ueda, H., Okawa, K., Iwamatsu, A., Handa, H., Hirose, S.
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Journal Title
Genes Dev. 17
Pages: 1605-1616
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Cloning of Cyc (Bmal-1) homolog in Bombyx mori ; Structural analyses and the tissue specific distributions.2003
Author(s)
Markova, E.P, Ueda, H., Sakamoto, K., Oishi, K., Shimada, Takeda, M.
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Journal Title
Comp.Biochem.Physiol.B. 134,10
Pages: 535-542
Description
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