Expansion and differentiation of marrow mesenchymal stem cells in porous ceramics

2004 Fiscal Year Final Research Report Summary

• Project/Area Number: 14208107
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
• Principal Investigator: OHGUSHI Hajime NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY (AIST), TISSUE ENGINEERING RESEARCH GROUP RESEARCH INSTITUTE FOR CELL ENGINEERING, GROUP LEADER, セルエンジニアリング研究部門, 研究グループ長 (80213669)
• Co-Investigator(Kenkyū-buntansha): ITO Atsuo NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY (AIST), 主任研究員 (30356480) ; KODAMA Makoto KYUSYU INSTITUTE OF TECHNOLOGY, GRADUATE SCHOOL OF LIFE SCIENCE AND SYSTEMS ENGINEERING, 大学院・生命体工学研究科, 助教授 (70344447) ; KANEMURA Yonehiro NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY (AIST), 研究員 (80344175)
• Project Period (FY): 2002 – 2004
• Keywords: Mesenchymal stem cells / Bone marrow / Bone regeneration / Tissue engineering / Culture / Hepatocyte / Neuron

Research Abstract

Stem cells have a remarkable potential for developing into many different cell types in the body. When a stem cell divides, each new cell has the ability to either remain a stem cell (self-renewal) or become another type of cell with a more specialized function (specific cell lineage commitment). Stem cells derived from postnatal animals (especially humans) are called adult stem cells and are generally thought to be multipotent stem cells.
Among adult stem cells, mesenchymal stem cells (MSCs) are also multipotent cells that can be isolated from adult bone marrow and can be induced in vitro and in vivo to differentiate into a variety of mesenchymal tissues, including bone and cartilage. Here, we report that we have succeeded to proliferate the number of MSCs from adult human as well as rat and also induced theses MSCs into osteo/chondrogenic lineage. Importantly, the osteogenic cells in the pore regions of various porous ceramics can be used to repair osseous defects and we have used the cells for the clinical applications.
Interestingly, the MSCs can also differentiate into hepatocyte and neuron. When MSCs were cultured in the presence of hepatocyte growth factor, the cells showed albumin and c-Kit expression. When the MSCs were cultured in the presence of mercapto-ethanol, the cells' morphorogy resembled neuron and showed Nestin expression. Therefore, MSCs derived from bone marrow can be used to regenerate hepatic and neural function. These results indicate the usefulness of MSCs for tissue engineering and, thus, the cells hold promise for utilization in regenerative medicine.

Research Products

• [Journal Article] Morphology of Porous Hydroxyapatite Scaffold for Hard Tissue Formation. (2005)
  • Author(s): Yoshikawa M, Tsuji N, Toda T, Machida H, Ohgushi H.
  • Journal Title: Key Eng Mater 17 Pages: 321-324
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Culturing Human Mesenchymal Stem Cells on Bioceramics for Hard Tissue Regeneration (Validation of In Vitro Bone Matrix Formed on Ceramics for Clinical Application). (2005)
  • Author(s): Ohgushi H, Kitamura S, Kotobuki N, Hirose M, Machida H, Oshima A, et al.
  • Journal Title: Key Eng Mater 17 Pages: 603-606
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] In Vitro Osteogenic Activity of Rat Mesenchymal Cells Cultured on Transparent β-Tricalcium Phosphate Ceramics. (2005)
  • Author(s): Kotobuki N, Ioku K, Kawagoe D, Nomura D, Fujimori H, Goto S et al.
  • Journal Title: Key Eng Mater 17 Pages: 663-666
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Comparison of Hard Tissue Formation in Two Porous Hydroxyapatite Scaffolds Treated with Hyaluronic Acid Sodium Salt. (2005)
  • Author(s): Tsuji N, Yoshikawa M, Toda T, Machida H, Ohgushi H.
  • Journal Title: Key Eng Mater 17 Pages: 961-964
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Comparison of Recombinant Growth/Differentiation Factor-5 (rGDF-5) and Bone Morphogenetic Protein-2 (rBMP-2) in the In Vivo Bone Formation in Porous Hydroxyapatite Ceramic. (2005)
  • Author(s): Shimaoka H, Dohi Y, Narikawa K, Ohgushi H, Ikeuchi M, Okamoto M, et al.
  • Journal Title: Key Eng Mater 17 Pages: 945-948
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Potentiation of the activity of bone morphogenetic protein-2 in bone regeneration by a PLA-PEG/hydroxyapatite composite. (2005)
  • Author(s): Kaito T, Myoui A, Takaoka K, Saito N, Nishikawa M, Tamai N, Ohgushi H, Yoshikawa H.
  • Journal Title: Biomaterials 26(1) Pages: 73-79
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Observation of osteogenic differentiation cascade of living mesenchymal stem cells on transparent hydroxyapatite ceramics. (2005)
  • Author(s): Kotobuki N, Ioku K, Kawagoe D, Fujimori H, Goto S, Ohgushi H.
  • Journal Title: Biomaterials 26(7) Pages: 779-785
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Role of metallothionein isoforms in bone formation processes in rat marrow mesenchymal stem cells in culture. (2005)
  • Author(s): Dohi Y, Shimaoka H, Ikeuchi M, Ohgushi H, Yonemasu K, Minami T.
  • Journal Title: Biol Trace Elem Res 104(1) Pages: 57-70
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Cartilage regeneration using mesenchymal stem cells and a three-dimensional poly-lactic-glycolic acid (PLGA) scaffold. (2005)
  • Author(s): Uematsu K, Hattori K, Ishimoto Y, Yamauchi J, Habata T, Takakura Y, et al.
  • Journal Title: Biomaterials 26(20) Pages: 4273-4279
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Tissue engineered ceramic artificial joint-ex vivo osteogenic differentiation of patient mesenchymal cells on total ankle joints for treatment of osteoarthritis. (2005)
  • Author(s): Ohgushi H, Kotobuki N, Funaoka H, Machida H, Hirose M, Tanaka Y, et al.
  • Journal Title: Biomaterials 26(22) Pages: 4654-4661
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Morphology of Porous Hydroxyapatite Scaffold for Hard Tissue Formation. (2005)
  • Author(s): Yoshikawa M, Tsuji N, Toda T, Machida H, Ohgushi H.
  • Journal Title: Key Eng Mater 284-286 Pages: 321-324
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Culturing Human Mesenchymal Stem Cells on Bioceramics for Hard Tissue Regeneration (Validation of In Vitro Bone Matrix Formed on Ceramics for Clinical Application). (2005)
  • Author(s): Ohgushi H, Kitamura S, Kotobuki N, Hirose M, Machida H, Oshima A, et al.
  • Journal Title: Key Eng Mater 284-286 Pages: 603-606
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Osteogenic Activity of Rat Mesenchymal Cells Cultured on Transparent 6-Tricalcium Phosphate Ceramics. (2005)
  • Author(s): Kotobuki N, Ioku K, Kawagoe D, Nomura D, Fujimori H, Goto S, et al.
  • Journal Title: Key Eng Mater 284-246 Pages: 663-666
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Comparison of Hard Tissue Formation in Two Porous Hydroxyapatite Scaffolds Treated with Hyaluronic Acid Sodium Salt. (2005)
  • Author(s): Tsuji N, Yoshikawa M, Toda T, Machida H, Ohgushi H.
  • Journal Title: Key Eng Mater 284-286 Pages: 961-964
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Comparison of Recombinant Growth/Differentiation Factor-5 (r-GDF-5) and Bone Morphogenetic Protein-2 (rBMP-2) in the In Vivo Bone Formation in Porous Hydroxyapatite Ceramic. (2005)
  • Author(s): Shimaoka H, Dohi Y, Narikawa K, Ohgushi H, Ikeuchi M, Okamoto M, et al.
  • Journal Title: Key Eng Mater 284-286 Pages: 945-948
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cartilage regeneration using mesenchymal stem cells and a three-dimensional poly-lactkrglycolic acid(PLGA) scaffold. (2005)
  • Author(s): Uematsu K, Hattori K, Ishimoto Y, Yamauchi J, Habata T, Takakura Y, et al.
  • Journal Title: Biomaterials 26(20) Pages: 4273-4279
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cultured autologous human cells for hard tissue regeneration : preparation and characterization of mesenchymal stem cells from bone marrow. (2004)
  • Author(s): Kotobuki, N., Hirose M., Takakura Y., Ohgushi, H
  • Journal Title: Artificial Organs 28(1) Pages: 33-39
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Osteogenic differentiation of human bone marrow-derived mesenchymal cells cultured on alumina ceramics. (2004)
  • Author(s): Kitamura, S., Ohgushi, H., Hirose, M., Funaoka, H, Takakura, Y., Ito, H.
  • Journal Title: Artificial Organs 28(1) Pages: 72-81
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Recombinant growth/differentiation factor-5 (GDF-5) stimulates osteogenic differentiation of marrow mesenchymal cells in porous hydroxyapatite ceramic. (2004)
  • Author(s): Shimaoka, H, Dohi, Y, Ohgushi, H., Ikeuchi, M., Okamoto, M., Kudo, A., Kirita, T., Yonemasu, K.
  • Journal Title: Journal of Biomedical Materials Research 68A(1) Pages: 168-176
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Osteogenic Activity of Human Marrow Cells on Alumina Ceramics. (2004)
  • Author(s): Tanaka Y, Ohgushi H, Kitamura S, Taniguchi A, Hayashi K, Isomoto S, Tohma Y, Takakura Y.
  • Journal Title: Key Eng Mater 16 Pages: 254-256
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Osteogenic potential of cryopreserved human bone marrow-derived mesenchymal cells after thawing in culture. (2004)
  • Author(s): Hirose M, Kotobuki N, Machida H, Kitamura S, Ohgushi H, Tateishi T.
  • Journal Title: Mat Sci Eng C-Bio S 2004 24(3) Pages: 335-359
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] In Vitro Osteogenic Activity of Rat Bone Marrow Derived Mesenchymal Stem Cells Cultured on Transparent Hydroxyapatite Ceramics. (2004)
  • Author(s): Noriko Kotobuki DK, Hirotaka Fujimori, Seishi Goto, Koji Ioku, Hajime Ohgushi.
  • Journal Title: Key Eng Mater 16 Pages: 1055-1058
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Three-dimensional visualization analysis of in vitro cultured bone fabricated by rat marrow mesenchymal stem cells. (2004)
  • Author(s): Kihara T, Oshima A, Hirose M, Ohgushi H.
  • Journal Title: Biochem Biophys Res Commun 3 Pages: 943-948
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Osteogenic potential of cryopreserved human bone marrow-derived mesenchymal cells after thawing in culture. (2004)
  • Author(s): Hirose M, Kotobuki N, Machida H, Kitamura S, Ohgushi H, Tateishi T.
  • Journal Title: Mat Sci Eng C-Bio S 24(3) Pages: 355-359
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Bone regeneration by grafting of cultured human bone. (2004)
  • Author(s): Yoshikawa T, Ohgushi H, Ichijima K, Takakura Y.
  • Journal Title: Tissue Eng 2004 10 Pages: 688-698
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Clinical application of marrow mesenchymal stem cells for hard tissue repair. (2004)
  • Author(s): Ohgushi H, Kitamura S, Kotobuki N, Hirose M, Machida H, Muraki K, Takakura Y.
  • Journal Title: Yonsei Med J 45 Pages: 61-67
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Which cartilage is regenerated, hyaline cartilage or fibrocartilage? Non-invasive ultrasonic evaluation of tissue engineered cartilage. (2004)
  • Author(s): Hattori K, Takakura Y, Ohgushi H, Habata T, Uematsu K, Takenaka M, Ikeuchi K.
  • Journal Title: Rheumatology (Oxford) 43(9) Pages: 1106-1108
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Ankle arthroplasty using three generations of metal and ceramic prostheses. (2004)
  • Author(s): Takakura Y, Tanaka Y, Kumai T, Sugimoto K, Ohgushi H.
  • Journal Title: Clin Orthop 2004 424 Pages: 130-136
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Growth factor combination for chondrogenic induction from human mesenchymal stem cell. (2004)
  • Author(s): Indrawattana N, Chen G, Tadokoro M, Shann LH, Ohgushi H, Tateishi T, Tanaka J, Bunyaratvej A.
  • Journal Title: Biochem Biophys Res Commun 320(3) Pages: 914-919
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Characterization of the cDNA encoding bullfrog, Rana catesbeiana, osteocalcin and two forms of the protein isolated from bone. (2004)
  • Author(s): Dohi Y, Tabata S, Yamaguchi M, Ohgushi H
  • Journal Title: Yonemasu K. Biochimie 86(7) Pages: 471-480
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Osteogenic potential of cultured bane/ceramic construct : comparison with marrow mesenchymal cell/ceramic composite. (2004)
  • Author(s): Iida J, Yoshikawa T, Akahane M, Ohgushi H, Dohi Y, Takakura Y, Nonomura A.
  • Journal Title: Cell Transplant 13(4) Pages: 357-365
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Bone tissue engineering using novel interconnected porous hydroxyapatite ceramics combined with marrow mesenchymal cells - Quantitative and three-dimensional image analysis -. (2004)
  • Author(s): Nishikawa M, Ohgushi H, Myoui A, Ikeuchi M, Tamai N, Yoshikawa H.
  • Journal Title: Cell Transplant 13(4) Pages: 367-376
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Enhancement of in vitro osteoblastic potential after selective sorting of osteoblasts with high alkaline phosphatase activity from human osteoblast-like cells. (2004)
  • Author(s): Kotobuki, N., Hirose, M., Funaoka, H., Ohgushi, H.
  • Journal Title: Cell Transplant 13(4) Pages: 377-383
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Chondrogenic differentiation of human mesenchymal stem cells cultured in a cobweb-like biodegradable scaffold. (2004)
  • Author(s): Chen G, Liu D, Tadokoro M, Hirochika R, Ohgushi H, Tanaka J, et al.
  • Journal Title: Biochem Biophys Res Commun 322(1) Pages: 50-55
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Cell adhesion of bone marrow cells, chondrocytes, ligament cells and synovial cells on a PLGA-collagen hybrid mesh. (2004)
  • Author(s): Chen G, Liu D, Maruyama N, Ohgushi H, Tanaka J, Tateishi T.
  • Journal Title: Mat Sci Eng C-Bio S 24(6-8) Pages: 701-926
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Cultured autologous human cells for hard tissue regeneration : preparation and characterization of mesenchymal stem cells from bone marrow. (2004)
  • Author(s): Kotobuki N, Hirose M, Takakura Y, Ohgushi H.
  • Journal Title: Artif Organs 28(1) Pages: 33-39
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Osteogenic differentiation of human bone marrow-derived mesenchymal cells cultured on alumina ceramics. (2004)
  • Author(s): Kitamura S, Ohgushi H, Hirose M, Funaoka H, Takakura Y, Ito H.
  • Journal Title: Artif Organs 28(1) Pages: 72-82
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Recombinant growth/differentiation factor-5(GDF-5) stimulates osteogenic differentiation of marrow mesenchymal stem cells in porous hydroxyapatite ceramic. (2004)
  • Author(s): Shimaoka H, Dohi Y, Ohgushi H, Ikeuchi M, Okamoto M, Kudo A, Kirita T, Yonemasu K.
  • Journal Title: J Biomed Mater Res 68A(1) Pages: 168-176
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Osteogenic Activity of Human Marrow Cells on Alumina Ceramics. (2004)
  • Author(s): Tanaka Y, Ohgushi H, Kitamura S, Taniguchi A, Hayashi K, Isomoto S, Tohma Y, Takakura Y.
  • Journal Title: Key Eng Mater 254-256 Pages: 181-184
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] In Vitro Osteogenic Potential of Cryopreserved/Thawed Human Bone Marrow-Derived Mesenchymal Stem Cells. (2004)
  • Author(s): Hirose M, Kotobuki N, Machida H, Kitamura S, Takakura Y, Ohgushi H.
  • Journal Title: Key Eng Mater 254-256 Pages: 1051-1054
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Osteogenic Activity of Rat Bone Marrow Derived Mesenchymal Stem Cells Cultured on Transparent Hydroxyapatite Ceramics. (2004)
  • Author(s): Noriko Kotobuki DK, Hirotaka Fujimori, Seishi Goto, Koji Ioku, Hajime Ohgushi
  • Journal Title: Key Eng Mater 254-256 Pages: 1055-1058
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Three-dimensional visualization analysis of in vitro cultured bone fabricated by rat marrow mesenchymal stem cells. (2004)
  • Author(s): Kihara T, Oshima A, Hirose M, Ohgushi H.
  • Journal Title: Biochem Biophys Res Commun 316(3) Pages: 943-948
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Bone regeneration by grafting of cultured human bone. (2004)
  • Author(s): Yoshikawa T, Ohgushi H, Ichijima K, Takakura Y.
  • Journal Title: Tissue Eng 10(5-6) Pages: 688-698
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Clinical application of marrow mesenchymal stem cells for hard tissue repair. (2004)
  • Author(s): Ohgushi H, Kitamura S, Kotobuki N, Hirose M, Machida H, Muraki K, Takakura Y.
  • Journal Title: Yonsei Med J 45 Suppl Pages: 61-67
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Which cartilage is regenerated, hyaline cartilage or fibrocartilage? Non-invasive ultrasonic evaluation of tissue-engineered cartilage. (2004)
  • Author(s): Hattori K, Takakura Y, Ohgushi H, Habata T, Uematsu K, Takenaka M, Ikeuchi K.
  • Journal Title: Rheumatology (Oxford) 43(9) Pages: 1106-1108
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Ankle arthroplasty using three generations of metal and ceramic prostheses. (2004)
  • Author(s): Takakura Y, Tanaka Y, Kumai T, Sugimoto K, Ohgushi H.
  • Journal Title: Clin Orthop (424) Pages: 130-136
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterization of the cDNA encoding bullfrog, Rana catesbeiana, osteocalcin and two forms of the protein isolated from bone. (2004)
  • Author(s): Dohi Y, Tabata S, Yamaguchi M, Ohgushi H, Yonemasu K.
  • Journal Title: Biochimie 86(7) Pages: 471-480
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Osteogenic potential of cultured bone/ceramic construct : comparison with marrow mesenchymal cell/ceramic composite. (2004)
  • Author(s): Iida J, Yoshikawa T, Akahane M, Ohgushi H, Dohi Y, Takakura Y, Nonomura A.
  • Journal Title: Cell Transplant 13(4) Pages: 357-365
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Bone tissue engineering using novel interconnected porous hydroxyapatite ceramics combined with marrow mesenchymal cells-Quantitative and three-dimensional image analysis-. (2004)
  • Author(s): Nishikawa M, Ohgushi H, Myoui A, Ikeuchi M, Tamai N, Yoshikawa H.
  • Journal Title: Cell Transplant 13(4) Pages: 367-376
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Transplantation of Hepatocytes Cultured on Hydroxyapatite into Nagase Analbuminemia Rats. (2003)
  • Author(s): Shinji Higashiyama, Megumi Noda, Satoko Muraoka, Motohiro Hirose, Hajime Ohgushi, Masaya Kawase, Kiyohito Yagi
  • Journal Title: Journal of Bioscience and Bioengineering 196 Pages: 83-85
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Osteogenic differentiation of cultured rat and human bone marrow cells on the surface of zinc-releasing calcium phosphate ceramics. (2003)
  • Author(s): Ikeuchi, M., Ito, A., Dohi, Y., Ohgushi, H., Shimaoka, H., Yonemasu, K., Tateishi, T.
  • Journal Title: Journal of Biomedical Materials Research 67A(4) Pages: 1115-1122
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] In-situ visualization and quantification of mineralization of cultured osteogenetic cells. (2003)
  • Author(s): Uchimura, E., Machida, H., Kotobuki, N., Kihara, T., Kitamura, S., Ikeuchi, M., Hirose, M., Miyake, J., Ohgushi, H.
  • Journal Title: Calcified Tissue International 73(6) Pages: 575-583
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Transplantation of Hepatocytes Cultured on Hydroxyapatite into Nagase Analbuminemia Rats (2003)
  • Author(s): Shinji Higashiyama, Megumi Noda, Satoko Muraoka, Motohiro Hirose, Hajime Ohgushi, Masaya Kawase, Kiyohito Yagi
  • Journal Title: Journal of Bioscience and Bioengineering Vol.96 No.1 Pages: 83-85
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Osteogenic differentiation of cultured rat and human bone marrow cells on the surface of zinc-releasing calcium phosphate ceramics. (2003)
  • Author(s): Ikeuchi, M., Ito, A., Dohi, Y., Ohgushi, H., Shimaoka, H., Yonemasu, K., Tateishi, T.
  • Journal Title: Journal of Biomedical Materials Research Part 67A(4) Pages: 1115-1122
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Recombinant Human Bone Morphogenetic Protein-2 Promotes Osteogenesis within Atelopeptide Type I Collagen Solution by Combination with Rat Cultured Marrow Cells. (2002)
  • Author(s): M.Ikeuchi, Y Dohi, K Horiuchi, H Ohgushi, T.Noshi, T.Yoshikawa, K.Yamamoto, M Sugimura
  • Journal Title: Journal of Biomedical Materials Research 60 Pages: 61-69
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Enhancing and suppressing effects of dexamethasone on transgene expression in vitro. (2002)
  • Author(s): M.Akahane, S.Kuriyama, H.Ohgushi, T.Akahane, K.Kawamura, S.Watanabe, F.Funaoka, H.Yoshiji, K.Ikenaka, Y.Takakura
  • Journal Title: International Journal of Molecular Medicine 10 Pages: 107-112
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Hydroxyapatite ceramics as a cariier of gene-transduced bone marrow cells. (2002)
  • Author(s): M.Akahane, H.Ohgushi, S.Kuriyama, T.Akahane, Y.Takakura
  • Journal Title: Journal of Orthopedic Science 17 No.6 Pages: 1677-1682
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Zinc-releasing calcium phosphate for stimulateing bone formation. (2002)
  • Author(s): A.Ito, H.Kawamura, M.Otsuka, M.Ikeuchi, H.Ohgushi, K Ishikawa, K Onuma, N Kanzaki, N.Ichinose
  • Journal Title: Materials Science and Engineering C 22 Pages: 21-25
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Recombinant Human Bone Morphogenetic Protein-2 Promotes Osteogenesis within Atelopeptide Type I Collagen Solution by Combination with Rat Cultured Marrow Cells. (2002)
  • Author(s): M.Ikeuchi, Y Dohi, K Horiuchi, H Ohgushi, T.Noshi, T.Yoshikawa, K.Yamamoto, M Sugimura
  • Journal Title: Journal of Biomedical Materials Research Vol.60 Pages: 61-69
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Enhancing and suppressing effects of dexamethasone on transgene expression in vitro. (2002)
  • Author(s): M.Akahane, S.Kuriyama, H.Ohgushi, T.Akahane, K.Kawamura, S.Watanabe, F.Funaoka, H.Yoshiji, K.Ikenaka, Y.Takakura
  • Journal Title: International Journal of Molecular Medicine Vol.10 Pages: 107-112
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Hydroxyapatite ceramics as a cariier of gene-transduced bone marrow cells. (2002)
  • Author(s): M.Akahane, H.Ohgushi, S.Kuriyama, T.Akahane, Y.Takakura
  • Journal Title: Journal of Orthopedic Science Vol.7 No.6 Pages: 677-682
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Zinc-releasing calcium phosphate for stimulateing bone formation. (2002)
  • Author(s): A.Ito, H.Kawamura, M.Otsuka, M.Ikeuchi, H.Ohgushi.K Ishikawa, K Onuma, N Kanzaki, N.Ichinose
  • Journal Title: Materials Science and Engineering C Vol.22 No.1 Pages: 21-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] Transactions of the Materials Research Society of Japan (2004)
  • Author(s): Hirose, M., Kotobuki, N., Machida, H., Kitamura, S., Takakura, Y., Ohgushi, H.
  • Total Pages: 2859-2862
  • Publisher: IUMRS-ICAM
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Bioceramics15 (2003)
  • Author(s): M.Hirose, N.Kotobuki, H.Machida, E.Uchimura, H.Ohgushi
  • Total Pages: 982(715-718)
  • Publisher: TRANS TECH PUBLICATIONS
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Bioceramics15 (2003)
  • Author(s): N.Kotobuki, M.Hirose, H.Funaoka, H.Ohgushi
  • Total Pages: 982(729-734)
  • Publisher: TRANS TECH PUBLICATIONS
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Bioceramics15 (2003)
  • Author(s): Ohgushi, M.Ikeuchi, T.Tateishi, Y.Tohma, T.Tanaka, T.Takakura
  • Total Pages: 982(651-654)
  • Publisher: TRANS TECH PUBLICATIONS
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Bioceramics15 (2003)
  • Author(s): Y.Thoma, H.Ohgushi, Y.Dohi, H.SHimaoka, Y.Ikeda, Y.Mizuta, T.Takakura
  • Total Pages: 982(615-618)
  • Publisher: TRANS TECH PUBLICATIONS
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Bioceramics15 (2003)
  • Author(s): Y.Tanaka, Y.Takakura, K.Kadono, A.Taniguchi, K.Hayashi, J.Iida, K.Sugimoto, Y.Thoma, H.Ohgushi
  • Total Pages: 982(805-808)
  • Publisher: TRANS TECH PUBLICATIONS
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Experimental and Clinical Reconstructive Microsurgery (2003)
  • Author(s): H.Ohgushi, M.Akahane
  • Total Pages: 5(70-74)
  • Publisher: Eds. Tamai, S., Springer-Verlag Tokyo
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Novartis Found Sympo.249 (2003)
  • Author(s): Ohgushi H, Miyake J, Tateishi T.
  • Total Pages: 251(118-127)
  • Publisher: John Wiley & Sons Ltd
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Biomaterials in Orthopedics (2003)
  • Author(s): Nishikawa, M., Ohgushi, H
  • Total Pages: 425-435
  • Publisher: Eds. Yaszemsk, MJ., Marcel Dekker Inc., New York, Basel
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Bioceramics14 (2002)
  • Author(s): M.Akahane, H.Ohgushi, S.Kuriyama, T.Akahane, K.Kawamura, Y.Takakura
  • Total Pages: 687(218-220)
  • Publisher: TRANS TECH PUBLICATIONS
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Bioceramics14 (2002)
  • Author(s): H.Ohgushi, T.Noshi, M.Ikeuchi, T.Yoshikawa, Y.Dohi, T.Tateishi
  • Total Pages: 687(218-220)
  • Publisher: TRANS TECH PUBLICATIONS
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Bioceramics (2002)
  • Author(s): H Ohgushi.
  • Total Pages: 24-27
  • Publisher: The McGraw-Hill Co., New York
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Recent Research Developments in Analytical Biochemistry (2002)
  • Author(s): Y.Dohi, H.Ohgushi, T.Minami, M Ikeuchi, K.Yonemasu
  • Total Pages: 95-106
  • Publisher: Scientific Research Flash, Vazhapalli India
  • Description: 「研究成果報告書概要(和文)」より