2003 Fiscal Year Final Research Report Summary
Function of stromal cell and follicular dendritic cells on the functional differentiation and formation of lymph node
| Project/Area Number |
14570007
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
General anatomy (including Histology/Embryology)
|
|---|
| Research Institution | TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY |
|---|
Principal Investigator |
YOSHIDA Toshiko TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY, ANATOMY, ASSOCIATE PROFESSOR, 医学部, 助教授 (00171421)
|
|---|
| Project Period (FY) |
2002 – 2003
|
| Keywords | IL-2 receptor / γ chain / knockout / mouse / stromal cell / Follicular dendritic cell(FDC) / immnohistochemistry / lymphoid organ |
|---|
| Research Abstract |
The relationship with stromal cells and follicular dendritic cells, which are considered to influence the structure of lymph node and the adjustment of microenvironment, was investigated used by IL-2 receptorγ chain knockout mouse.
We had observed the ultrastructure by the electron microscopy. And the peculiar characteristic for the cell was detected by using monoclonal antibody, that is MOMA-1,MOMA-2,TCR-β chain,CD45R,MIDC-8,FDC-M1 and FDC-M2, for immuno histochemistry.
1.The IL-2 Rγ chain did not lead the decrease the numbers of TcRβ(+) T cells, but decrease CD45R(+) B cells in the lymph node and spleen. It dose not influence the MIDC-8 positive dendritic cells which were located in the T cell area even if the structure of lymph node is not complete. The numbers of monocyte were increased, but MOMA-1(+) cells in spleen were disappeared, but much ones in lymph node. Precursor of FDCs was included in a group of stromal cells in this mouse. Because after the stimulation by the antigen, there was observed typical FDC elongated cytoplasmic processes with antigens between lymphocytes. The B cell did not play critical role to differentiate from immature FDC to mature.
2.The stromal cells separated from IL-2R KO mouse were cultured with memory T cell, memory B cell and immune complexes. When we used the method of the separation FDC from immature lymph node which is difficult to separate from T cell areas and B cell area, we could get the precursor cells
Our result suggested that IL-2 receptorγ chain may do not play critical roles on the differentiation of FDC. This model is very useful for investigation of functional construction and microenvironment of stromal cells and FDC.
|
