2003 Fiscal Year Final Research Report Summary
Establishment of a novel therapy for glioma invasion by antisense inhibition of the targeting molecule.
| Project/Area Number |
14571314
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Cerebral neurosurgery
|
|---|
| Research Institution | Ehime University |
|---|
Principal Investigator |
OHNISHI Takanori Ehime University, School of Medicine, professor, 医学部, 教授 (70233210)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HARADA Hironobu Ehime University, University Hospital, Assistant, 医学部附属病院, 助手 (20335897)
NAKAGAWA Kou Ehime University, University Hospital, Assistant professor, 医学部附属病院, 講師 (50155678)
|
|---|
| Project Period (FY) |
2002 – 2003
|
| Keywords | GLIOMA / TUMOR INVASION / LAMININ-8 / ANTISENSE |
|---|
| Research Abstract |
In order to clarify the molecular mechanism of glioma invasion, we studied an expression of laminin-8 in brain tumors and investigated an effect of antisense inhibition of laminin-8 on migration and invasion of glioma cells. The protein and mRNA of laminin-8 were highly expressed in malignant gliomas, particulary in glioblastomas showing diffuse invasion. Antisense of laminin-8(α4 subunit) markedly inhibited the glioma cell migration and invasion in vitro without affecting the cell growth. In addtion, antisense of laminin-8 significantly inhibited the glioma cell invasion in both a rat brain slice model and a tumor-inoculated model. Antisense inhibition of laminin-8 resulted in weak formation of actin stress fibers in glioma cells but did not affect the expression of Rac and Cdc42. These results suggest that gene suppression of laminin-8 may inhibit the glioma cell motility through the inhibition of formation of focal adhesion.
|
Research Products
(10 results)
