2003 Fiscal Year Annual Research Report

カルボニル還元酵素による卵巣がん転移抑制の分子機構

Research Project

Project/Area Number	14657054
Research Institution	Hirosaki University
Principal Investigator	土田成紀弘前大学, 医学部, 教授 (20142862)
Co-Investigator(Kenkyū-buntansha)	早狩誠弘前大学, 医学部, 助教授 (80156421)
Keywords	卵巣がん / 転移 / カルボニル還元酵素 / 血管内皮細胞増殖因子 / hypoxia-inducible factor
Research Abstract	カルボニル還元酵素発現によるマウス卵巣がん細胞の性状変化の解析昨年度、マウスカルボニル還元酵素(CR)の発現ベクターを作製し、細胞にトランスフェクトして、CRタンパク質の発現を調べたが、低かったので、今年度、新たな発現ベクターに変更し、これにCR cDNAを挿入し、CR発現ベクターを作製した。これをマウス卵巣がん細胞にリポフェクトアミンを用いて導入した。トランスフェクトした細胞を10%ウシ胎児血清を含むDMEM培地で培養した。細胞数はMTT法に準ずる方法で測定し、細胞形態は位相差顕微鏡で観察した。CRと血管内皮細胞増殖因子(VEGF)の発現をウエスタンブロット法で検討した。本発現ベクターにより卵巣がん細胞でCRの強い発現を認めた。CR発現に伴い、細胞増殖や細胞形態に明らかな変化を認めなかったが、VEGFの発現は低下することが明らかになった。来年度以降、本細胞系を用いてCRによるVEGFの発現抑制の機序、CRを発現させたがん細胞では転移が抑制されるかなどを検討する予定である。

Research Products

(6 results)

All Other

All Publications (6 results)

[Publications] Mayama, Jun: "Polymorphic glutathione S-transferase subunit 3 of rat liver exhibits different susceptibilities to carbon tetrachloride : differences in their interactions with heat-shock protein 90"Biochemical Journal. 372(2). 611-616 (2003)
[Publications] Hayakari, Makoto: "Kinetic-controlled hydrolysis of Leu-Val-Val-hemorphin-7 catalyzed by angiotensin-converting enzyme from rat brain"Peptides. 24(7). 1075-1082 (2003)
[Publications] Aizawa, Shu: "Characterization of cell death induced by ethacrynic acid in a human colon cancer cell line DLD-1 and suppression by N-acetyl-L-cysteine"Cancer Science. 94(10). 886-893 (2003)
[Publications] Chang, Tung-Huei: "Expression of MRP2 and MRP3 during liver regeneration after 90% partial hepatectomy in rats"Transplantation. 77(1). 22-27 (2004)
[Publications] Kato, Masashi: "Induction of differentiation and peroxisome proliferator-activated receptor gamma expression in colon cancer cell lines by troglitazone"Journal of Cancer Research and Clinical Oncology. (印刷中). (2004)
[Publications] Takahata, Takenori: "Inhibition of 3T3-L1 adipocyte differentiation by 6-ethoxyzolamide : repressed peroxisome proliferator-activated receptor gamma mRNA and enhanced CCAAT/enhancer binding protein beta mRNA levels"Biochemical Pharmacology. (印刷中). (2004)

2003 Fiscal Year Annual Research Report

カルボニル還元酵素による卵巣がん転移抑制の分子機構

Principal Investigator

土田 成紀 弘前大学, 医学部, 教授 (20142862)

Research Products

[Publications] Mayama, Jun: "Polymorphic glutathione S-transferase subunit 3 of rat liver exhibits different susceptibilities to carbon tetrachloride : differences in their interactions with heat-shock protein 90"Biochemical Journal. 372(2). 611-616 (2003)

[Publications] Hayakari, Makoto: "Kinetic-controlled hydrolysis of Leu-Val-Val-hemorphin-7 catalyzed by angiotensin-converting enzyme from rat brain"Peptides. 24(7). 1075-1082 (2003)

[Publications] Aizawa, Shu: "Characterization of cell death induced by ethacrynic acid in a human colon cancer cell line DLD-1 and suppression by N-acetyl-L-cysteine"Cancer Science. 94(10). 886-893 (2003)

[Publications] Chang, Tung-Huei: "Expression of MRP2 and MRP3 during liver regeneration after 90% partial hepatectomy in rats"Transplantation. 77(1). 22-27 (2004)

[Publications] Kato, Masashi: "Induction of differentiation and peroxisome proliferator-activated receptor gamma expression in colon cancer cell lines by troglitazone"Journal of Cancer Research and Clinical Oncology. (印刷中). (2004)

[Publications] Takahata, Takenori: "Inhibition of 3T3-L1 adipocyte differentiation by 6-ethoxyzolamide : repressed peroxisome proliferator-activated receptor gamma mRNA and enhanced CCAAT/enhancer binding protein beta mRNA levels"Biochemical Pharmacology. (印刷中). (2004)

土田成紀弘前大学, 医学部, 教授 (20142862)