2007 Fiscal Year Final Research Report Summary
Development of functional cell scaffolds and bioreactors for regenerative medicine based on stem cells
| Project/Area Number |
15100009
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kyoto University |
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Principal Investigator |
TABATA Yasuhiko Kyoto University, Institute for Frontier Medical Sciences, Professor (50211371)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Masaya Kyoto University, Institute for Frontier Medical Sciences, Assistant Professor (10332735)
UMEZAWA Akihiro Kyoto University, National Research Institute for Child Health and Development, Department of Reproductive Biology, Director (70213486)
YAMAOKA Tetsuji Kyoto University, National Cardiovascular Research Institute, Advance Medical Engineering Center, Director (50243126)
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| Project Period (FY) |
2003 – 2007
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| Keywords | functional scaffolds / stem cells / proliferation and differentiation / bioreactor / biodegradable polymer materials / sponges / non-woven fabrics |
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| Research Abstract |
The objective of this research is to investigate and develop biomedical technologies and methodologies for the enhancement of stem cells proliferation and differentiation which is necessary to realize tissue regeneration therapy. Various 3-dimensional sponges and non-woven fabics of cell scaffold have been designed and prepared from different biodegradable polymers while the compatibility of scaffolds to cells is modified and improved by incorporating cell adhesion substances into them to and create functional scaffolds. In addition, bioreactor technologies are combined with the functional scaffold to promote the proliferation and differentiation of stem cells in vitro. The results obtained are as follows: The mechanical reinforcement of scaffolds by the incorporation of fibers, the surface modification of scaffolds with the self-assemble technology of alkanethiols, the improvement of scaffold cytocompatibility by the immobilization of cell adhesion substances, the promotion of cells proliferation and differentiation in vitro by combining the scaffold with various bioreactors. It is highly expected that all the results strongly contribute not only to the research acceleration of basic biology and medicine, but also the future development of tissue regeneration therapy.
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Research Products
(162 results)
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[Journal Article] Cell attachment to pet films coated with a thermo-sensitive blockco-polymer with different chemical composition2007
Author(s)
Takamoto, T., Yasuda, K., Tsujino, T., Sugihara, S., Kanaoka, S., Aoshima, S., Tabata. Y.
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Journal Title
J. Biomater. Sci. Polymer Edn 18(9)
Pages: 1211-1222
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Cell attachment to pet films coated with a thermo-sensitive block co-polymer with different chemical composition2007
Author(s)
Takamoto, T., Yasuda, K., Tsujino, T., Sugihara, S., Kanaoka, S., Aoshima, S., Tabata. Y.
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Journal Title
J. Biomater. Sci. Polymer Edn 18(9)
Pages: 1211-1222
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Reversal of mouse hepatic failure using an implanted liver-assistdevice containing ES eell-derived hepatocytes.2006
Author(s)
Soto-Gutierrez A., Kobayashhi N., Rivas-Carrillo J. D., Navarro-AlvarezN., Zhao D., Okitsu T., Noguchi H., Basma H., Tabata Y., Chen Y., TanakaK., Narushima M., MikiA., Ueda T., Jun H. -S., Tanaka N., Lebkowski J., Yoon J. -W., Fox I. J.
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Journal Title
Nature biotechnology 24
Pages: 1412-1419
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Evaluation of bone regeneration by DNA release from composites of oligo(poly(ethylene glycol) fumarate) and cationized gelatin microspheres in a critical-sized calvarial defect.2006
Author(s)
Kasper, F. K., Young, S., Tanahashi, K., Barry, M. A., Tabata, Y., Jansen, J. A., Mikos, A. G.
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Journal Title
J Biomed Mater Res 78A
Pages: 335-342
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Reversal of mouse hepatic failure using an implanted liver-assist device containing ES cell-derived hepatocytes.2006
Author(s)
Soto-Gutierrez A., Kobayashhi, N., Rivas-Carrillo J. D., Navarro-Alvarez N., Zhao D., Okitsu T., Noguchi H., Basma H., Tabata Y.. Chen Y, Tanaka K., Narushima M., Miki A., Ueda T., Jun H.-S., Tanaka N., Lebkowski J., Yoon J.-W., Fox I. J.
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Journal Title
Nature biotechnology 24
Pages: 1412-1419
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Effect culture substrate and fibroblast growth factor addition on theproliferation and differentiation of human adipo stromal cells.2005
Author(s)
Inoue, S., Hori, Y., Hirano, Y., Inamoto, T., Tabata, Y.
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Journal Title
J. Biomater. Sci., Polym. Eds. 16(1)
Pages: 57-77
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Impregnation of plasmid DNA into three-dimensional scaffolds andmedium perfusion enhance in vitro DNA expression of mesenchymalstem cells.2005
Author(s)
Hosseinkhani, H., Inatsugu, Y., Hiraoka, Y., Inoue, S., Shimokawa, H., Tabata. Y.
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Journal Title
Tissue Eng. 11(9/10)
Pages: 1459-1475
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Proliferation and differentiation of rat bone marrow stromal cells onpoly (glycolic acid)-collagen sopnge.2005
Author(s)
Fujita, M., Kinoshita, Y., Sato, E., Maeda, H., Ozono, S., Negishi, H., Kawase, T., Hiraoka, Y., Takamoto, T., Tabata Y Kamevama Y
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Journal Title
Tissue Eng. 11(9/10)
Pages: 1346-1355
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Effect culture substrate and fibroblast growth factor addition on the proliferation and differentiation of human adipo stromal cells.2005
Author(s)
Inoue, S., Hori, Y, Hirano, Y, Inamoto, T., Tabata, Y.
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Journal Title
J. Biomater. Sci., Polym. Eds. 16(1)
Pages: 57-77
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Impregnation of plasmid DNA into three-dimensional scaffolds and medium perfusion enhance in vitro DNA expression of mesenchymal stem cells.2005
Author(s)
Hosseinkhani, H., Inatsugu, Y., Hiraoka, Y., Inoue, S., Shimokawa, H., Tabata, Y.
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Journal Title
Tissue Eng. 11(9/10)
Pages: 1459-1475
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Proliferation and differentiation of rat bone marrow stromal cells on poly(glycolic acid)-collagen sopnge.2005
Author(s)
Fujita, M., Kinoshita, Y, Sato, E., Maeda, H., Ozono, S., Negishi, H., Kawase, T., Hiraoka, Y, Takamoto, T., Tabata, Y, Kameyama, Y.
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Journal Title
Tissue Eng. 11(9/10)
Pages: 1346-1355
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Novel collagen sponge reinforced with polyglycolic acid fiber producesrobust, normal hair in murine hair reconstitution model.2004
Author(s)
Itoh, M., Hiraoka, Y., Kataoka, K., Huh, NH., Tabata, Y., Okochi, H.
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Journal Title
Tissue Eng. 10(5-6)
Pages: 818-824
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] A custom-shaping system for bone regeneration by seedingmarrow stromal cells onto a web-like biodegradable hybrid sheet.2004
Author(s)
Tsuchiya, K., Mori, T., Chen, G., Ushida, T., Tateishi, T., Matsuno, T., Sakamoto, M., Umezawa A
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Journal Title
Cell Tissue Res. 70
Pages: 415-418
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Novel collagen sponge reinforced with polyglycolic acid fiber produces robust, normal hair in murine hair reconstitution model.2004
Author(s)
Itoh, M., Hiraoka, Y, Kataoka, K, Huh, NH., Tabata, Y, Okochi, H.
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Journal Title
Tissue Eng. 10(5-6)
Pages: 818-824
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A custom-shaping system for bone regeneration by seeding marrow stromal cells onto a web-like biodegradable hybrid sheet.2004
Author(s)
Tsuchiya, K, Mori, T., Chen, G., Ushida, T., Tateishi, T., Matsuno, T., Sakamoto, M., Umezawa, A.
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Journal Title
Cell Tissue Res. 70
Pages: 415-418
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Mechanism for the phase transition of a genetically engineeredelastin model peptide (VPGIG)_<40> in aqueous solution2003
Author(s)
Yamaoka, T., Tamura, T., Seto, Y., Tada, T., Kunugi, S., D. A. Tirrell
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Journal Title
Biomacromolecules 4
Pages: 1680-1685
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Mechanism for the phase transition of a genetically engineered elastin model peptide (VPGIG)4o in aqueous solution2003
Author(s)
Yamaoka, T., Tamura, T., Seto, Y, Tada, T., Kunugi, S., D. A. Tirrell
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Journal Title
Biomacromolecules 4
Pages: 1680-1685
Description
「研究成果報告書概要(欧文)」より
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[Presentation] In situ regeneration of adipose tissue by combining a collagen sponge with controlled release of bFGF2003
Author(s)
Hiraoka, Y., Yamashiro, H., Yasuda, K., Takamoto, T., Kimura, Y., Inamoto, S., Tabata, Y.
Organizer
24th annual meeting of the Japanese Society of Inflammation and Regeneration
Place of Presentation
Kyoto
Year and Date
20031126-20031127
Description
「研究成果報告書概要(欧文)」より
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[Book] Biomaterial Science2003
Author(s)
Ishihara, K., Hatanaka, K., Yamaoka. T.. Oya, Y
Total Pages
206
Publisher
Tokyo Kagaku Dojin Co. Ltd.
Description
「研究成果報告書概要(欧文)」より
