2004 Fiscal Year Final Research Report Summary
Establishment of array-based CGH technology and its use for-diagnosis of genetic diseases in clinical setting
| Project/Area Number |
15390112
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human genetics
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
INAZAWA Johji Tokyo Medical and Dental University, Medical Research Institute, Department of Molecular Cytogenetics, Professor, 難治疾患研究所, 教授 (30193551)
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| Co-Investigator(Kenkyū-buntansha) |
IMOTO Issei Tokyo Medical and Dental University, Medical Research Institute, Department of Molecular Cytogenetics, Associate Professor, 難治疾患研究所, 助教授 (30258610)
YOKOI Sana Tokyo Medical and Dental University, Medical Research Institute, Department of Molecular Cytogenetics, Research Associate, 難治疾患研究所, 助手 (30372452)
MIZUTANI Syuki Tokyo Medical and Dental University, Graduate School, Pediatrics and Developmental Biology, Professor, 大学院・医歯学総合研究科, 教授 (60126175)
ARII Shigeki Tokyo Medical and Dental University, Graduate School, Hepato-Biliary Pancreatic Surgery, Professor, 大学院・医歯学総合研究科, 教授 (50151171)
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| Project Period (FY) |
2003 – 2004
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| Keywords | CGH / Genetic disease / Chromosome aberration / BAC / PAC / DNA methylation / Autism / contiguous gene syndrome |
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| Research Abstract |
Comparative genomic hybridization (CGH) has already made a significant impact on cancer cytogenetics. However, CGH to metaphase chromosomes can provide only limited resolution at 5-10 Mb level. To circumvent this limitation, array-based CGH has been devised. We have constructed different types of BAC-based CGH-arrays. The first consisted of 〜4500 BACs for genome-wide analysis (named MCG Whole Genome Array-4500) ; this array provided a resolution of 〜0.7Mb. The second consisted of 〜800 BACs harboring 800 known cancer-relaed genes, intended for diagnosis of cancer-specific copy-number aberrations (MCG Cancer Array-800, see URL : http://www.cghtmd.jp/cghdatabase/index.html). The third of our arrays contains 212 contiguous BACs spanning a 〜20-Mb region at 1p36 (MCG 1p36 Contig Array). Furthermore, to accomplish high-throughput screening for methylated sites in the whole genome, we have combined array-CGH with MCA, and our preliminary data show that this "BAC array-based MCA (BAMCA)" can discriminate BAC clones that harbor methylated CpGs on an array platform. Our custom-made CGH-arrays paves the way for identification of disease-related genetic aberrations that have not yet been detected by existing technologies, and our array-based CGH technology should soon be practical for diagnosis of cancer or genetic diseases in clinical settings.
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[Book] 学術月報2004
Author(s)
稲澤 譲治
Total Pages
88
Publisher
独立行政法人日本学術振興会
Description
「研究成果報告書概要(和文)」より
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