Molecular epidemiological study on blood-borne protozoan parasites of small ruminants in China

2005 Fiscal Year Final Research Report Summary

• Project/Area Number: 15405036
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: HOKKAIDO UNIVERSITY (2005); Obihiro University of Agriculture and Veterinary Medicine (2003-2004)
• Principal Investigator: SUGIMOTO Chihiro Hokkaido University, Research Center for Zoonosis Control, Professor, 人獣共通感染症リサーチセンター, 教授 (90231373)
• Co-Investigator(Kenkyū-buntansha): FUJISAKI Kozo Obihiro University of Agriculture and Veterinary Medicine, National Research Center for Protozoan Diseases, Professor, 原虫病研究センター, 教授 (00292095) ; XUAN Xuenan Obihiro University of Agriculture and Veterinary Medicine, National Research Center for Protozoan Diseases, Professor, 原虫病研究センター, 教授 (10292096) ; INOUE Noboru Obihiro University of Agriculture and Veterinary Medicine, National Research Center for Protozoan Diseases, Associate Professor, 原虫病研究センター, 助教授 (10271751) ; YOKOYAMA Naoaki Obihiro University of Agriculture and Veterinary Medicine, National Research Center for Protozoan Diseases, Associate Professor, 原虫病研究センター, 助教授 (80301802)
• Project Period (FY): 2003 – 2005
• Keywords: Theileria / Babesia / Trypanosoma / China / protozaoa / small ruminant

Research Abstract

Hemoprotozaon parasite closely related to Chinese Theileria species in Sheep was detected from ticks, Haemaphysalis japonicum infesting on Japanese serow and blood samples of serow by using PCR targeting small subunit ribosomal RNA gene of Chinese Theileria sp. from sheep. However, its infectivity to splenectomized sheep was not confirmed when blood from infected serow was injected. Therefore, transmission of Theileria parasites between domestic and wild ruminants was considered no to occur under natural condition.
Several methods based on loop-mediated DNA amplification (LAMP) have been developed for diagnoses of diseases caused by hemoprotozoan parasites, including equine Babesia/Trypanosoma, ovine Babesia/Theileria. Specificity and sensitivity of these tests had been demonstrated to be high as compared with the results obtained by PCR tests. Infection rate of Trypanosoma evansi among horses in Xinjian area exceeded 90 % as determined by LAMP. These LAMP methods will be useful for molecular epidemiological studies of hemoprotozoan parasites in China.

Research Products

• [Journal Article] Molecular characterization of Theileria orientalis piroplasma protein encoded by an open reading frame(To ORF2) in a genomic fragment. (2005)
  • Author(s): Kim, J-Y.
  • Journal Title: J. Vet. Med. Sci. 66・8 Pages: 957-963
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Expression of a gene encoding Trypanosoma congolense putative abc1 family protein is developmentally regulated. (2005)
  • Author(s): Baticados, W.
  • Journal Title: J. vet. Med. Sci. 67・2 Pages: 157-164
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Theileria orientalis : Cloning a cDNA encoding a protein similar to thiol protease with haemoglobin-binding activity. (2005)
  • Author(s): He W
  • Journal Title: Exp Parasitol. 111・3 Pages: 143-153
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Evaluation of loop-mediated isothermal amplification (LAMP), PCR and parasitological tests for detection of Trypanosoma evansi in experimentally infected pigs. (2005)
  • Author(s): Thekisoe, O.
  • Journal Title: Vet. Parasitol. 130・3-4 Pages: 327-330
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Detection of antibodies to Neospora caninum in cattle by enzyme-linked immunosorbent assay with truncated NcSRS2 expressed in Escherichia coli. (2005)
  • Author(s): Gaturaga I
  • Journal Title: J Parasitol 91・1 Pages: 191-192
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Molecular characterization of Theileria orientalis piroplasma protein encoded by an open reading frame (To ORF2) in a genomic fragment. (2005)
  • Author(s): Kim, J-Y., Yokoyama, N., Kumar, S., Inoue, N., Fujisaki, K., Sugimoto, C.
  • Journal Title: J.Vet.Med.Sci. 66-8 Pages: 957-963
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of a gene encoding Trypanosoma congolense putative abc1 family protein is developmentally regulated. (2005)
  • Author(s): Baticados, W., Witola, W.H., Inoue, N., Kim J-Y., Kuboki, N., Xuan, X., Yokoyama, N., Sugimoto
  • Journal Title: J.Vet.Med.Sci., 2005 67-2 Pages: 157-164
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Theileria orientalis : Cloning a cDNA encoding a protein similar to thiol protease with haemoglobin-binding activity. (2005)
  • Author(s): He W, Ohashi K, Sugimoto C, Onuma M.
  • Journal Title: Exp Parasitol. 111-3 Pages: 143-153
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Evaluation of loop-mediated isothermal amplification (LAMP), PCR and parasitological tests for detection of Trypanosoma evansi in experimentally infected pigs. (2005)
  • Author(s): Thekisoe, O.M.M., Inoue, N., Kuboki, N., Tuntasuvan, D., Bunnoy, W., Borisutsuwan, S., Igarashi, I., Sugimoto, C.
  • Journal Title: Vet.Parasitol. 130-3-4 Pages: 327-330
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Detection of antibodies to Neospora caninum in cattle by enzyme-linked immunosorbent assay with truncated NcSRS2 expressed in Escherichia coll. (2005)
  • Author(s): Gaturaga I, Chahan B, Xuan X, Huang X.Liao M, Fukumoto S, Hirata H, Nishikawa Y, Takashima Y, Suzuki H, Fujisaki K, Sugimoto C.
  • Journal Title: J Parasitol. 91-1 Pages: 191-192
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Molecular epidemiological survey of benign Theileria parasites of cattle in Japan : detection of a new type of major piroplasm surface protein gene. (2004)
  • Author(s): Kim, J-Y.
  • Journal Title: J. Vet. Med. Sci. 66・3 Pages: 251-256
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Inhibitory effect of antiserum to surface antigen P50 of Babesia gibsoni on growth of parasites in severe combined immunodeficiency mice given canine red blood cells. (2004)
  • Author(s): Fukumoto, S.
  • Journal Title: Infect. Immun. 72・3 Pages: 1795-1798
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Expression of Babesia equi EMA-1 and EMA-2 during merozoite developmental stages in erythrocyte and their interaction with erythrocytic membrane skeleton. (2004)
  • Author(s): Kumar, S.
  • Journal Title: Mol. Biochem. Parasitol. 133・2 Pages: 221-227
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Growth-inhibitory effect of heparin on Babesia parasites. (2004)
  • Author(s): Bork, S
  • Journal Title: Antimicrob. Agents Chemother. 48・1 Pages: 236-241
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification of a piroplasm protein of Theileria orientalis that binds to bovine erythrocyte band 3. (2004)
  • Author(s): Kim, J-Y.
  • Journal Title: Mol. Biochem. Parasitol. 137・2 Pages: 193-200
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Molecular epidemiological survey of benign Theileria parasites of cattle in Japan : detection of a new type of major piroplasm surface protein gene. (2004)
  • Author(s): Kim, J-Y., Yokoyama, N., Kumar, S., Inoue, N., Yamaguchi, T., Sentoku, S., Fujisaki, K., Sugimoto, C.
  • Journal Title: J.Vet.Med.Sci. 66-3 Pages: 251-256
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Inhibitory effect of antiserum to surface antigen P50 of Babesia gibsoni on growth of parasites in severe combined immunodeficiency mice given canine red blood cells. (2004)
  • Author(s): Fukumoto, S., Xuan, X., Takabatake, N., Igarashi, I., Sugimoto, C., Fujisaki, K., Nagasawa, H., Mikami, T., Suzuki, H.
  • Journal Title: Infect.Immun. 72-3 Pages: 1795-1798
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of Babesia equi EMA-1 and EMA-2 during merozoite developmental stages in erythrocyte and their interaction with erythrocytic membrane skeleton. (2004)
  • Author(s): Kumar, S., Yokoyama, N., Kim, J-Y., Huang, X., Inoue, N., Xuan, X., Igarashi, I., Sugimoto, C.
  • Journal Title: Mol.Biochem.Parasitol. 133-2 Pages: 221-227
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Growth-inhibitory effect of heparin on Babesia parasites. (2004)
  • Author(s): Bork, S., Yokoyama, N., Ikehara, Y., Kumar, S., Sugimoto, C., Igarashi, I.
  • Journal Title: Antimicrob.Agents Chemother. 48-1 Pages: 236-241
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of a piroplasm protein of Theileria orientalis that binds to bovine erythrocyte band 3. (2004)
  • Author(s): Kim, J-Y., Yokoyama, N., Kumar, S., Inoue, N., Inaba, M., Fujisaki, K., Sugimoto, C.
  • Journal Title: Mol.Biochem.Parasitol. 137-2 Pages: 193-200
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Serodiagnosis of Neospora caninum infection in cattle by enzyme-linked immunosorbent assay with recombinant truncated NcSAG1. (2003)
  • Author(s): Chahan, B
  • Journal Title: Vet Parasitol. 118・3-4 Pages: 177-185
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Cloning and expression of mitochondrial heat shock protein 70 of Trypanosoma congolense and potential use as a diagnostic antigen. (2003)
  • Author(s): Bannai, H.
  • Journal Title: Clin, Diagn. Lab. Immunol. 10・5 Pages: 926-933
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] High-level expression of truncated surface antigen P50 of Babesia gibsoni in insect cells by baculovirus and evaluation of its immunogenicity and antigenicity. (2003)
  • Author(s): Fukumoto, S.
  • Journal Title: Clin. Diagn. Lab. Immunol. 10・4 Pages: 596-601
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Loop-mediated isothermal amplification for detection of African trypanosomes. (2003)
  • Author(s): Kuboki, N
  • Journal Title: J. Clin. Microbiol. 41・12 Pages: 5517-5524
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Molecular characterization of a gene encoding a 29-kDa cytoplasmic protein of Babesia gibsoni and evaluation of its diagnostic potentiality. (2003)
  • Author(s): Fukumoto, S.
  • Journal Title: Mol. Biochem. Parasitol. 131・2 Pages: 129-136
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Serodiagnosis of Neospora caninum infection in cattle by enzyme-linked immunosorbent assay with recombinant truncated NcSAG1 (2003)
  • Author(s): Chahan B, Gaturaga I, Huang X, Liao M, Fukumoto S, Hirata H, Nishikawa Y, Suzuki H, Sugimoto C, Nagasawa H, Fujisaki K, Igarashi I, Mikami T, Xuan X.
  • Journal Title: Vet Parasitol. 118-3-4 Pages: 177-185
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cloning and expression of mitochondrial heat shock protein 70 of Trypanosoma congolense and potential use as a diagnostic antigen. (2003)
  • Author(s): Bannai, H., Sakurai, T., Inoue, N., Sugimoto, C, Igarashi, I.
  • Journal Title: Clin.Diagn.Lab.Immunol. 10-5 Pages: 926-933
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] High-level expression of truncated surface antigen P50 of Babesia gibsoni in insect cells by baculovirus and evaluation of its immunogenicity and antigenicity (2003)
  • Author(s): Fukumoto, S., Xuan, X., Kadota, K., Igarashi, I., Sugimoto, C., Fujisaki, K., Nagasawa, H., Mikami, T., Suzuki, H.
  • Journal Title: Clin.Diagn.Lab.Immunol. 10-4 Pages: 596-601
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Loop-mediated isothermal amplification for detection of African trypanosomes. (2003)
  • Author(s): Kuboki, N., Inoue, N., Sakurai, T., Di Cello, F., Grab, D.J., Suzuki, H., Sugimoto, C., Igarashi, I.
  • Journal Title: J.Clin.Microbiol. 41-12 Pages: 5517-5524
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Molecular characterization of a gene encoding a 29-kDa cytoplasmic protein of Babesia gibsoni and evaluation of its diagnostic potentiality. (2003)
  • Author(s): Fukumoto, S., Xuan, X., Inoue, N., Igarashi, I., Sugimoto, C., Fujisaki, K., Nagasawa, H., Mikami, T., Suzuki, H.
  • Journal Title: Mol.Biochem.Parasitol. 131-2 Pages: 129-136
  • Description: 「研究成果報告書概要(欧文)」より