2004 Fiscal Year Final Research Report Summary
the role of adaptor protein SH2-B in adipocyte differentiation
| Project/Area Number |
15590522
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | Ritsumeikan University |
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Principal Investigator |
SUZUKI Kenji Ritsumeikan University, Department of Information Science and Engineering, Professor, 情報理工学部, 教授 (10187726)
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| Project Period (FY) |
2003 – 2004
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| Keywords | adipocyte / tyrosine kinase / adaptor protein / signal transduction |
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| Research Abstract |
Expression of SH2-B splicing variants in 3T3-L1 cells during adipocyte differentiation.
Preadipocyte cell line mouse 3T3-L1 is extensively used to investigate the molecular mechanisms in adipogenesis. To evaluate adipocyte differentiation, three different methods such as Oil red-O staining, RT-PCR for adipocyte specific genes and GPDH assay, were used. Among 4 isoforms of SH2-B, expression of β- andγ- isoforms were detected in 3T3-L1 cells, and these expression levels were up-regulated during adipogenesis. Expression of SH2-B family proteins was also investigated. Changes in expression level of APS was similar to these of SH2-B splicing variants, although mRNA level of lnk did not change during adipogenesis.
Effect of overexpression or suppression of SH2-B in 3T3-L1 cells on adipocyte differentiation.
Willd-type and mutated human SH2-Bβ were introduced into 3T3-L1 cells by using a lipofection method. The expression of human SH2-B was confirmed by RT-PCR and western blot analysis. The expression of wild-type SH2-B accelerated the cellular differentiation into adipocytes. On the other hand, the mutated SH2-B, which lacks the ability to bind to phosphorylated proteins, suppressed its differentiation. The expression of small interfering RNA against SH2-B also inhibited adipogenesis in 3T3-L1 cells.
In conclusion, these results clearly indicate that SH2-B plays a pivotal role in adipocyte differentiation.
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Research Products
(12 results)
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[Journal Article] Genetic variation in hypoxia-inducible factor 1-alpha and its possible association with high altitude adaptation in Sherpas.2003
Author(s)
Suzuki, K., Kizaki, T., Hitomi, Y., Nukita, M., Kimoto, K., Miyazawa, N., Kobayashi, K., Ohnuki, Y., Ohno, H.
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Journal Title
Med.Hypotheses 61(3)
Pages: 385-389
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Gliclazide protects pancreatic beta-cells from damage by hydrogen peroxide.2003
Author(s)
Kimoto, K., Suzuki, K., Kizaki, T., Hitomi, Y., Ishida, H., Katsuta, H., Itoh, E., Ookawara, T., Suzuki, K., Honke, K., Ohno, H.
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Journal Title
Biochem.Biophys.Res.Commun. 303
Pages: 112-119
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Effects of Nutrient and Food Intake on Calcaneous Bone Mass among Healthy Japanese Women in the Predelivery and Postpartum Periods.2003
Author(s)
Sekine, Y., Matsunaga, N., Kokaze, A., Yoshida, M., Suzuki, K., Ohno, H., Takashima, Y.
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Journal Title
J.Women Health 12(7)
Pages: 643-653
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Intermittent Hypobaric Hypoxia Increases the Ability of Neutrophils to Generate Superoxide Anion in Humans.2003
Author(s)
Hitomi, Y, Miyamura, M., Mori, S., Suzuki, K., Kizaki, T., Itoh, C., Murakami, K., Haga, S., Ohno, H.
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Journal Title
Clin.Exp.Pharmacol.Physiol. 30(9)
Pages: 659-664
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Physical Exercise and Extracellulrar Superoxide Dismutase : Antiatherogenic Effects.2003
Author(s)
Ohno, H., Kizaki, T., Suzuki, K., Hitomi, Y., Watanabe, S., Ookawara, T., Suzuki, K., Haga, S., Saitoh, D., Radak, Z., Ji, L.L.
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Journal Title
Adv.Exerc.Sports Physiol. 9(2)
Pages: 45-53
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Effect of Eccentric Exercise on Neutrophil Function in Human Plasma.2003
Author(s)
Miyazaki, H., Haga, S., Suzuki, K., Toshinai, K., Esaki, K., Oh-ishi, S., Hitomi, Y., Kizaki, T., Ji, L.L., Ohno, H.
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Journal Title
Adv.Exerc.Sports Physiol. 9(2)
Pages: 69-75
Description
「研究成果報告書概要(欧文)」より
