2004 Fiscal Year Final Research Report Summary
Establishment of the rat glioma model at the midbrain and screenig of the tumor suppressor function
| Project/Area Number |
15591523
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | National University Corporation Tottori University |
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Principal Investigator |
KAMITANI Hideki National University Corporation Tottori University, Faculty of Medicine, Associated Professor, 医学部, 助教授 (70194967)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Takashi National University Corporation Tottori University, Faculty of Medicine, Professor, 医学部, 教授 (00175100)
TABUCHI Sadaharu National University Corporation Tottori University, Faculty of Medicine, Research Associate, 医学部, 助手 (40314603)
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| Project Period (FY) |
2003 – 2004
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| Keywords | midbrain glioma / experimental brain tumor / C6 glioma / stereotactic implantation |
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| Research Abstract |
As the treatment of human intrinsic brainstem gliomas remains challenging, experimental glioma models are needed. We developed a rat model of intrinsic brain stem glioma that uses a stereotactic frame to fix the head for the delivery of C6 glioma cells to target sites via a permanently implanted cannula. We inoculated the rat midbrain, pons, or cerebral cortex with 5 x 10^4 cells suspended in 1 μl of culture medium over the course of 2 min. Three days post-implantation, tumor formation was visible in the periaqueductal gray matter in the midbrain and in the tegmentum of the pons. On the 10^<th> day, the tumor diameter exceeded over 2 mm ; length, there was no tumor-cell seeding into the cerebrospinal fluid space. The tumor manifested the histological features typical of glioblastoma ; Ki-67 labeling index was 32%. Because in our model the cannula is permanently implanted, additional inocula can be delivered. Finally, we establish the reproductive glioma model at the brain stem including midbrain with stereotactically accuracy.
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