Elucidation of molecular mechanism of transcription-coupled nucleotide excision repair

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H02820
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Osaka University
• Principal Investigator: SAIJO Masafumi 大阪大学, 生命機能研究科, 准教授 (90221986)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ヌクレオチド除去修復 / コケイン症候群 / 紫外線高感受性症候群 / ユビキチン化 / SUMO化 / UV

Outline of Final Research Achievements

We analyzed transcription-coupled nucleotide excision repair (TC-NER), a subpathway of nucleotide excision repair that rapidly removes transcription-blocking DNA damage, and the following results on the functions of TC-NER factors were obtained. (1) The C-terminal region and SUMOylation of CSB plays critical roles in TC-NER. (2) UVSSA is degraded by proteasome upon dissociation from USP7, resulting in TC - NER deficiency, but when a mutation is introduced at the ubiquitination site, degradation is suppressed and TC - NER becomes normal. (3) Ubiquitination of RNA polymerase II may be involved in the restart of transcription after removal of transcription-blocking DNA damage. These results are important cues to reveal molecular mechanism of TC-NER.

Free Research Field

分子生物学