Development of platelet generation system through waved miRNA regulation

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H03005
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Kyoto University
• Principal Investigator: ETO KOJI 京都大学, iPS細胞研究所, 教授 (50286986)
• Co-Investigator(Renkei-kenkyūsha): ARAI Fumihito 名古屋大学, 工学研究科(研究院), 教授 (90221051)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 血小板 / バイオリアクター / ずり応力 / 大量培養 / iPS細胞 / 巨核球

Outline of Final Research Achievements

We aimed to establish a novel "megakaryocyte maturation / platelet release system" that enables to generate bona fide platelets with the same function as in vivo with high efficiency. Based on the live bone marrow observation of mouse megakaryocytes protruding their cytoplasm into sinusoid blood vessels and producing platelets under blood flow stimulation, we analyzed flow vectors in those images. The results indicated that turbulence in the blood flow are present adjacent to the cell wall of megakaryocytes from which platelet production event is continuously observed. We then prepared a new model culture tank that demonstrated new mechanisms of how turbulence regulates remodeling of megakaryocytes and promotes platelet production. Using this model, we found conditions that enabled to produce 100 billion platelets by 8L-scale tank. These findings led to creating a new research area of a turbulent flow-controlled thrombopoiesis mechanism.

Free Research Field

幹細胞、再生医療