Structural analysis of membrane protein based on multi-labeled sites

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H03119
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemical biology
• Research Institution: University of Toyama
• Principal Investigator: TOMOHIRO Takenori 富山大学, 大学院医学薬学研究部(薬学), 教授 (70357581)
• Co-Investigator(Kenkyū-buntansha): 千葉 順哉 富山大学, 大学院医学薬学研究部(薬学), 助教 (50436789)
• Co-Investigator(Renkei-kenkyūsha): SHIMIZU Takahiro 富山大学, 大学院医学薬学研究部(薬学), 准教授 (40353437) ; MATSUNAGA Shigeru 浜松ホトニクス株式会社, 中央研究所, 専任部員 (40391198)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 化学プローブ / 光アフィニティーラベル / 結合部位解析 / 分子認識 / 標的同定

Outline of Final Research Achievements

Multifunctionalization of photocross-linker including fluorogenicity and cleavability has improved efficiency of labeled site analysis and enabled simultaneous analysis of multiple trace amount labels. We have developed a new methodology to monitor the allosteric structural change of protein due to binding of substrates based on label amount change of each labeled amino acid within the ligand-binding domain.

Free Research Field

ケミカルバイオロジー