Multimodal Digital Holographic Microscopy for Simultaneous Measurement of 3D Fluorescence and 3D Phase

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H03580
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Optical engineering, Photon science
• Research Institution: Kobe University
• Principal Investigator: MATOBA OSAMU 神戸大学, システム情報学研究科, 教授 (20282593)
• Co-Investigator(Renkei-kenkyūsha): MIZUKAMI Tamio 長浜バイオ大学, バイオサイエンス学部, 教授 (80367896)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: マルチモーダルディジタルホログラフィック顕微鏡 / 蛍光 / 位相

Outline of Final Research Achievements

In this research, we proposed a multimodal digital holographic microscopy (DHM) that can realize simultaneous measurement of three-dimensional (3D) fluorescence and 3D phase imaging. We fabricated a multimodal DHM that can obtain simultaneously two-dimensional fluorescence distribution and 3D phase distribution. The effectiveness of the proposed DHM was confirmed by the experimental results of plant cells and mouse's brain cells. Furthermore, for the development of single shot measurement of 3D fluorescence distribution, we proposed the common-path off-axis incoherent DHM and its feasibility was confirmed by the experiment.

Free Research Field

計測光学