Creation of micro-RNA-encapsulated artificial viral capsids bearing cell-penetrating ability

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H03838
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: Tottori University
• Principal Investigator: MATSUURA Kazunori 鳥取大学, 工学研究科, 教授 (60283389)
• Co-Investigator(Kenkyū-buntansha): 岩崎 崇 鳥取大学, 農学部, 准教授 (30585584) ; 三浦 典正 鳥取大学, 医学部, 准教授 (30325005)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 人工ウイルスキャプシド / 細胞透過性ペプチド / 自己集合 / 核酸医薬 / 細胞内導入

Outline of Final Research Achievements

We have developed peptide nanocapsules (artificial viral capsids) self-assembled from beta-annulus peptide from tomato bushy stunt virus. In this research project, we developed nucleic acids-encapsulated artificial viral capsids bearing cell-penetrating peptides. We succeeded in synthesizing beta-annulus peptide bearing cell-penetrating His16 peptide at C-terminal, and encapsulating ssDNA into the artificial viral capsids modified with His16. The His16-modified artificial viral capsids were internalized well into HT1080 cells, whereas unmodified capsids hardly internalized. In addition, we have developed encapsulation of ssDNA via disulfide bonds into artificial viral capsids.

Free Research Field

生体高分子化学