Mutli-Omics single cell analysis

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04318
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genome biology
• Research Institution: The University of Tokyo
• Principal Investigator: Suzuki Yutaka 東京大学, 大学院新領域創成科学研究科, 教授 (40323646)
• Co-Investigator(Kenkyū-buntansha): 土原 一哉 国立研究開発法人国立がん研究センター, 先端医療開発センター, 分野長 (00415514) ; 山下 理宇 東北大学, 東北メディカル・メガバンク機構, 准教授 (10401259) ; 河野 隆志 国立研究開発法人国立がん研究センター, 研究所, 分野長 (80280783)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 多層オーミクス解析 / シングルセル解析

Outline of Final Research Achievements

In this study, we have attempted to develop a series of methods by which single cell multi-omics analysis is enabled. We have successfully developed a suite of single cell analytical methods for transcriptional start sites analysis and open chromatin analysis, even though single cell DNA methylation analysis was not enabled mainly due to the difficulties in strict control of the bisulfite reaction. We have applied the developed method to monitor the diverse expression regulations between individual cells. We described fluctuating transcriptional responses invoked by the anti-cancer drugs either in susceptible or resistant lung cancer cell lines. We further examined transcriptome data from clinical specimen using a public cancer genome database and found that similar response seems to also occur in vivo. In addition to applying the developed method for our own purposes, we provided it to a number of researchers via collaborations.

Free Research Field

ゲノム科学