Revisiting "the multi-tubulin hypothesis" through comprehensive analysis of tubulin isotypes

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04369
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Tohoku University
• Principal Investigator: Sugimoto Asako 東北大学, 生命科学研究科, 教授 (80281715)
• Co-Investigator(Renkei-kenkyūsha): KUBOTA Yukihiko 東北大学, 大学院生命科学研究科, 助教 (70333325)
• Research Collaborator: HARUTA Nami 東北大学, 大学院生命科学研究科, 博士研究員 ; HONDA Yu 東北大学, 大学院生命科学研究科, 大学院生 ; TSUCHIYA Kenta 東北大学, 大学院生命科学研究科, 大学院生 ; OBINATA Hiroyuki 東北大学, 大学院生命科学研究科, 大学院生
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 微小管 / チューブリンアイソタイプ / 線虫

Outline of Final Research Achievements

In this study, we tested the "multi-tubulin hypothesis" in which multiple tubulin isotypes contribute to the diversity of microtubule dynamics, using the nematode C. elegans as a model system. Using genome editing, all tubulin isotype genes (9 alpha and 6 beta genes) were systematically knocked out and tagged with GFP, and their expression patterns and loss-of-function phenotypes were analyzed. Each isotypes were expressed in specific cell types, and ectopic expression experiments proved that composition of tubulin isotypes contribute to the diversity of microtubule dynamics.

Free Research Field

細胞生物学