2017 Fiscal Year Final Research Report
Molecular physiology of tissue circuit for cold acclimation
| Project/Area Number |
15H04404
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Chromosome dynamics
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| Research Institution | Konan University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | C. elegans / 低温馴化 / フィードバック制御 / 精子 / 線虫 |
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| Outline of Final Research Achievements |
To investigate temperature acclimation, we are using cold acclimation in Caenorhabditis elegans. 20°C-cultivated animals did not survival at 2°C. In contrast, 15°C cultivated animals can survive. Previously reported, this cold acclimation is regulated by ASJ temperature sensing-neuron, which releases insulin that is received by intestine and neuron. To identify genes in downstream of insulin-signaling, we performed DNA microarray analysis. We found that expressions of sperm genes were changed in insulin receptor mutants, and sperm mutants showed abnormal cold acclimation. Genetic epistasis analysis suggested that abnormal cold acclimation of sperm mutant was suppressed by the mutations in ASJ. Calcium imaging analysis showed that ASJ neuronal activity in response to temperature was decreased in sperm mutant gsp-4, and rescued by expressing gsp-4 gene in sperm. Thus, we propose a novel feedback between sperm and ASJ temperature sensing-neuron in cold acclimation of C. elgans.
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| Free Research Field |
分子神経遺伝学
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