2017 Fiscal Year Final Research Report
Understanding of PCB-degrading soil bacterium and development of experimental methods for applied microbiology
| Project/Area Number |
15H04471
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | MMLV-RT / tailing activity / tailing enhancers / adaptor ligation / catabolite control / protein acetylation / TCRS |
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| Outline of Final Research Achievements |
To investigate a PCB degrader Acidovorax sp. KKS102, a method named qTnSeq was developed. We revealed strong 3' tailing activity of a reverse transcriptase, and a series of tailing enhancers. We also optimized an unique reaction named CIS reaction. In the CIS reaction, a single strand DNA with 3' end sequence complementary to the tail is incorporated with concomitant DNA polymerization starting from the 3' tail. We designed and created transposon cassette for qTnSeq, and constructed a Tn mutant library consisting of about 60,000 clones, and we demonstrated the usefulness of the qTnSeq method. In addition, we investigated Acidovorax sp. KKS102 with respect to its catabolite control mechanism and a mobile genetic element that carries PCB-degrading genes.
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| Free Research Field |
分子生物学
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