Does prostaglandin become the insect gwowth control factor?

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04611
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Insect science
• Research Institution: Kyushu University
• Principal Investigator: Yamamoto Koji 九州大学, 農学研究院, 助教 (00346834)
• Co-Investigator(Kenkyū-buntansha): 坪田 拓也 国立研究開発法人農業生物資源研究所, その他部局等, 主任研究員 (00612772) ; 有竹 浩介 第一薬科大学, 薬学部, 教授 (70390804) ; 東浦 彰史 大阪大学, たんぱく質研究所, 助教 (90598129)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: プロスタグランジン / プロスタグランジン合成酵素

Outline of Final Research Achievements

Knowledge on role and synthesis of prostaglandins (PGs) in insect are limited. PGE synthase (PGES) and PGD synthase (PGDS) were already identified, and three-dimensional structure of PGES was determined.
In this study, we examined physiological role of PG by using genome-editing, TALEN, and X-ray analysis. In knock-out mutant of PGES, PG was not present by using mass-spectrometry. Since we were interested in composition of the active sites in PGDS, we determined crystal structure of PGDS. Based on the PGDS structure and site-directed mutagenesis, we identified the amino acid residues (Tyr8、 Lue14、Trp39、Lys43、Gln50、Val51、Gln63、and Ser64) involved in catalytic function.

Free Research Field

応用昆虫