2017 Fiscal Year Final Research Report
Chemically reactive oligonucleotides for site-specific modification of RNA and artificial RNA editing
| Project/Area Number |
15H04633
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemical pharmacy
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
TANIGUCHI Yosuke 九州大学, 大学院薬学研究院, 准教授 (00452714)
FUCHI Yasufumi 九州大学, 大学院薬学研究院, 助教 (40748795)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 遺伝子発現制御 / RNA / DNA / 人工核酸 / オリゴヌクレオチド / 部位特異的RNA修飾 |
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| Outline of Final Research Achievements |
This study was aimed at the development of the oligodeoxynucleotides to selectively modify RNA with a high base- and sequence selectivity, and their application to regulate gene the translation.As a result, a selective acylation of the 2’-hydroxy group of the RNA molecules has been achieved by the reactive oligonucleotide incorporating a new nucleobase analog as a catalyst of the acyl transfer.In addition, new nucleoside analogs have been contracted with the indolin skeleton, which were incorporated into the oligonucleotides for the selective acylation of the amino group of RNA bases by the activation of photo-irradiation.n order to evaluate the effect of the modification of RNA base, non-cell translation system has been established based on the use of synthesized mRNA, in which produced peptides are easily analyzed by gel-analysis as well as MS measurement. In conclusion, this study has successfully established oligonucleotide basis for the artificial regulation of gene expression.
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| Free Research Field |
核酸化学
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