Development of diagnostic method for atherosclerosis targeting adipokine binding proteins

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04762
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Osaka University
• Principal Investigator: Kihara Shinji 大阪大学, 医学系研究科, 教授 (20332736)
• Co-Investigator(Kenkyū-buntansha): 山本 浩靖 大阪大学, 医学系研究科, 准教授 (00631201)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 蛋白質 / 医療・福祉 / 分析科学 / 細胞・組織 / 生体分子

Outline of Final Research Achievements

Based on the combination of immunoprecipitation and mass spectrometry analysis of human serum and cultured human cells, we identified E-selectin ligand (ESL)-1and Mac2 Binding Protein (M2BP) as novel adiponectin binding proteins.
We investigated that adiponectin has anti-atherosclerotic function through the inhibition of monocyte adhesion on endothelium via direct association between adiponectin and ESL-1 on monocyte. In addition, we found that serum adiponectin-M2BP complex levels were markedly higher in patients with coronary artery disease (CAD) than in healthy subjects, and M2BP abrogated the anti-atherogenic effects of adiponectin on cultured endothelial cells. Moreover, we elucidated that serum adiponectin-cystatin C complex levels associated with coronary plaque vulnerability evaluated by intravascular ultrasound in CAD patients.

Free Research Field

臨床検査医学